MYH6 Antibody (940344) [Unconjugated]

MYH6 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MYH6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Western blot shows lysates of human heart atrium. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH6 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 940344
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MYH6 Antibody (940344) [Unconjugated] Summary

• Immunogen: Human MYH6 peptide
  Accession # P13533
• Specificity: Detects human MYH6. The peptide immunogen sequence represents a region specific to MYH6 and not present in other MYH isoforms.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MYH6 Antibody (940344) [Unconjugated]

• alpha-MHC
• ASD3
• cardiomyopathy
• CMD1EE
• CMH14
• Hypertrophic 1
• MYH6
• MYHC
• MYHCA
• MyHC-Alpha
• Myosin Heavy Chain 6
• Myosin Heavy Chain, Cardiac Muscle Alpha Isoform
• Myosin, Heavy Chain 6, Cardiac Muscle, Alpha
• Myosin, Heavy Polypeptide 6, Cardiac Muscle, Alpha (Cardiomyopathy, Hypertrophic 1)
• Myosin-6
• SSS3

Background

The MYH6 gene encodes the alpha chain of cardiac myosin, a muscle motor protein with two regions that bind actin at aa 657-679 and 759-773. Defects in this gene cause atrial septal defect 3, hypertrophic cardiac myopathy and sick sinus syndrome. MYH6 participates in embryonic and adult heart development and muscle contraction.  In adult heart, the beta chain isoform predominates in the ventricular tissue, while both isoforms are found in variable proportions in atrial tissue.  Force development is much faster in atrial tissue and ATP consumption is much higher.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYH6 Antibody (MAB8979)(4)

Publications using MAB8979

• Abraham, E;Kostina, A;Volmert, B;Roule, T;Huang, L;Yu, J;Williams, AE;Megill, E;Douglas, A;Pericak, OM;Morris, A;Stronati, E;Larrinaga-Zamanillo, A;Fueyo, R;Zubillaga, M;Andrake, MD;Akizu, N;Aguirre, A;Estaras, C; A retinoic acid:YAP1 signaling axis controls atrial lineage commitment Cell reports 2025-05-07 [PMID: 40343798] (Immunocytochemistry, Human)
• Lin, CY;Ching, YY;Wu, SF;Lee, YK;Fan, HC;Su, LY;Tsai, SY;Chen, YC;Shen, CI;Su, HL; Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells Cell transplantation 2023-09-12 [PMID: 37698258] (ICC, Human)
• HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting Stem Cell Research, 2022-04-04;61(0):102779. 2022-04-04 [PMID: 35427904] (ICC, Human)
• LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo Cell Stem Cell, 2019-03-07;0(0):. 2019-03-07 [PMID: 30853557] (IHC, Mouse)

Bioinformatics

• Gene Symbol: MYH6
• Uniprot:
  • P13533()