Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).

• Reactivity: Hu
• Applications: ELISA, Flow, CyTOF-ready
• Clone: MPO421-8B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free Summary

• Description: Novus Biologicals Mouse Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free (NBP2-41406) is a monoclonal antibody validated for use in ELISA and Flow. Anti-Myeloperoxidase/MPO Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human myeloperoxidase
• Specificity: The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1-4 ug/ml
• Application Notes: Use in FLOW cytometry reported in scientific literature ( PMID 27355490). This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (NBP2-41406)(1)

Publication using NBP2-41406

• van der Spek AH, Bloise FF, Tigchelaar W et al. The Thyroid Hormone Inactivating Enzyme Type 3 Deiodinase is Present in Bactericidal Granules and the Cytoplasm of Human Neutrophils Endocrinology 2016-08-01 [PMID: 27355490] (FLOW, Human)

Bioinformatics

• Gene Symbol: MPO