Myeloperoxidase/MPO Antibody (392105) [Unconjugated]

Images

 
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line and human neutrophil. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # ...read more
Myeloperoxidase/MPO was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using 8 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) for 3 hours at room ...read more
Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) overnight at 4 °C. Before ...read more
Myeloperoxidase/MPO was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Myeloperoxidase/MPO Mono­clonal Antibody (Catalog # MAB3174) at 10 µg/mL for 3 hours ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
392105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Myeloperoxidase/MPO Antibody (392105) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Myeloperoxidase/MPO
Val279-Ser745
Accession # P05164
Specificity
Detects human Myeloperoxidase/MPO in Western blots. No cross-reactivity with recombinant human Eosinophil Peroxidase is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MPO
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB3174 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody (392105) [Unconjugated]

  • EC 1.11.1
  • EC 1.11.1.7
  • Lactoperoxidase
  • MPO
  • Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myeloperoxidase/MPO Antibody (MAB3174)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Gerbil.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(2)
Western Blot
(1)
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Human
(2)
Mouse
(2)
Gerbil
(1)
All Species

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Video Protocols

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Bioinformatics

Gene Symbol MPO
Uniprot