Myeloperoxidase/MPO Antibody (392105) [Unconjugated]

Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line and human neutrophil. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myeloperoxidase/MPO at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Myeloperoxidase/MPO was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using 8 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) for 3 hours at room temperature. Cells were stained (red) and counter­stained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Myeloperoxidase/MPO was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Myeloperoxidase/MPO Mono­clonal Antibody (Catalog # MAB3174) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju­gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of mouse spleen using Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 392105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Myeloperoxidase/MPO Antibody (392105) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Myeloperoxidase/MPO
  Val279-Ser745
  Accession # P05164
• Specificity: Detects human Myeloperoxidase/MPO in Western blots. No cross-reactivity with recombinant human Eosinophil Peroxidase is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody (392105) [Unconjugated]

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (MAB3174)(9)

Publications using MAB3174

• F Peng, J Xie, H Liu, Y Zheng, X Qian, R Zhou, H Zhong, Y Zhang, M Li Shifting focus from bacteria to host neutrophil extracellular traps of biodegradable pure Zn to combat implant centered infection Bioactive materials, 2023;21(0):436-449. 2023 [PMID: 36185738] (ELISA Capture, Mouse)
• JS O'Connell, B Li, A Zito, A Ahmed, M Cadete, N Ganji, E Lau, M Alganabi, N Farhat, C Lee, S Eaton, R Mitchell, S Ray, P De Coppi, K Patel, A Pierro Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells PLoS ONE, 2021;16(12):e0260522. 2021 [PMID: 34855833] (IHC, Human)
• V Puhl, H Bonnekoh, J Scheffel, T Hawro, K Weller, P von den Dr, HJ Röwert-Hub, J Cardoso, M Gonçalo, M Maurer, K Krause A novel histopathological scoring system to distinguish urticarial vasculitis from chronic spontaneous urticaria Clinical and translational allergy, 2021;11(2):e12031. 2021 [PMID: 33949135] (IHC, Human)
• C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma Journal of personalized medicine, 2021;11(2):. 2021 [PMID: 33578644] (IHC, Human)
• C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia Sci Rep, 2018;8(1):18062. 2018 [PMID: 30584250] (IHC, Mouse)
• X Zhang, L Wei, J Wang, Z Qin, J Wang, Y Lu, X Zheng, Q Peng, Q Ye, F Ai, P Liu, S Wang, G Li, S Shen, J Ma Suppression Colitis and Colitis-Associated Colon Cancer by Anti-S100a9 Antibody in Mice Front Immunol, 2017;8(0):1774. 2017 [PMID: 29326691] (IHC-P, Mouse)
• H Hosoda, K Nakamura, Z Hu, H Tamura, J Reich, K Kuwahara-A, T Iba, Y Tabe, I Nagaoaka Antimicrobial cathelicidin peptide LL?37 induces NET formation and suppresses the inflammatory response in a mouse septic model Mol Med Rep, 2017;0(0):. 2017 [PMID: 28849130] (Human)
• Sahraoui A, Dewachter C, de Medina G, Naeije R, Aouichat Bouguerra S, Dewachter L Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils. PLoS ONE, 2016;11(2):e0148117. 2016 [PMID: 26840416] (IHC-P, Gerbil)
• Ero-Tolliver I, Hudson B, Bhave G The Ancient Immunoglobulin Domains of Peroxidasin Are Required to Form Sulfilimine Cross-links in Collagen IV. J Biol Chem, 2015;290(35):21741-8. 2015 [PMID: 26178375] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MPO
• Uniprot:
  • P05164()