Myeloperoxidase/MPO Antibody (358621) [Unconjugated]

Recombinant Human Myeloperoxidase/MPO protein was serially diluted 2-fold and captured by Rat Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB31741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 358621
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Myeloperoxidase/MPO Antibody (358621) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived human Myeloperoxidase/MPO protein
  Ala49-Ser745
  Accession # P05164
• Specificity: Detects human Myeloperoxidase/MPO in sandwich ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody (358621) [Unconjugated]

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P05164()