TFB2M Antibody

Western Blot: TFB2M Antibody [NBP1-78283] - (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TFB2M Antibody [NBP1-78283] - 5ug/ml staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TFB2M Antibody Summary

• Immunogen: Peptide with sequence KHCFGRRSATVIDH corresponding to internal region according to NP_071761.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFB2M
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1-0.3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 ug/ml
• Application Notes: Western blot: Approx 45kDa band observed in Human Lung lysates (calculated MW of 45.3kDa according to NP_071761.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Colon.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFB2M Antibody

• dimethyladenosine transferase 2, mitochondrial
• EC 2.1.1.-
• FLJ22661
• FLJ23182
• HCV NS5A-transactivated protein 5
• Hepatitis C virus NS5A-transactivated protein 5
• Hkp1
• h-mtTFB
• h-mtTFB2
• hTFB2M
• Mitochondrial 12S rRNA dimethylase 2
• Mitochondrial transcription factor B2
• mtTFB2
• NS5ATP5
• S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
• transcription factor B2, mitochondrial

Background

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA atthe conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction withPOLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, itactivates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFB2M Antibody (NBP1-78283)(1)

Publication using NBP1-78283

• Litonin D, Sologub M, Shi Y et al. Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. J Biol Chem 2010-06-11 [PMID: 20410300]

Bioinformatics

• Gene Symbol: TFB2M