KLHL1 Antibody

Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 ug/mL.

Immunohistochemistry-Paraffin: KLHL1 Antibody [NBP1-76670] - Rat brain tissue with KLHL1 antibody at 10 ug/ml.

Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in Human brain cell lysate with KLHL1 antibody using NBP1-76670 at (A) 2 and (B) 4 ug/ml.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ELISA, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

KLHL1 Antibody Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1. The immunogen is located within the first 50 amino acids of KLHL1.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLHL1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 mg/ml.
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for KLHL1 Antibody

• FLJ30047
• kelch (Drosophila)-like 1
• kelch-like 1 (Drosophila)
• KIAA1490kelch-like protein 1
• Mayven-related protein 2
• MRP2

Background

The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLHL1
• Uniprot:
  • AAF81719(Human)