KLHL1 Antibody

Images

 
Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 ug/mL.
Immunohistochemistry-Paraffin: KLHL1 Antibody [NBP1-76670] - Rat brain tissue with KLHL1 antibody at 10 ug/ml.
Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in Human brain cell lysate with KLHL1 antibody using NBP1-76670 at (A) 2 and (B) 4 ug/ml.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

KLHL1 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1. The immunogen is located within the first 50 amino acids of KLHL1.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLHL1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 mg/ml.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for KLHL1 Antibody

  • FLJ30047
  • kelch (Drosophila)-like 1
  • kelch-like 1 (Drosophila)
  • KIAA1490kelch-like protein 1
  • Mayven-related protein 2
  • MRP2

Background

The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for KLHL1 Antibody (NBP1-76670)

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Diseases for KLHL1 Antibody (NBP1-76670)

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Bioinformatics

Gene Symbol KLHL1
Uniprot