Mre11 Antibody (15B8.1E7.6) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clone
15B8.1E7.6
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Janelia Fluor 646

Order Details

Mre11 Antibody (15B8.1E7.6) [Janelia Fluor® 646] Summary

Immunogen
Mre11 Antibody (15B8.1E7.6) [Janelia Fluor 646] was made to a partial recombinant mouse Mre11 protein (amino acids 68-608) [UniProt Q61216]
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
MRE11
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Flow (Intracellular)
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Mre11 Antibody (15B8.1E7.6) [Janelia Fluor® 646]

  • ATLDmeiotic recombination (S. cerevisiae) 11 homolog A
  • AT-like disease
  • DNA recombination and repair protein
  • double-strand break repair protein MRE11A
  • endo/exonuclease Mre11
  • HNGS1
  • Meiotic recombination 11 homolog 1
  • Meiotic recombination 11 homolog A
  • MRE11 homolog 1
  • MRE11 homolog A
  • MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
  • Mre11
  • MRE11A
  • MRE11B

Background

Mre11 (double-strand break repair protein MRE11A) is an important component of MRN/X complex (Mre11, Rad50, and Nbs1/Xrs2), playing a central role in double-strand break (DSB) repair, DNA recombination, telomere integrity maintenance, meiotic cell division, and viral infection. Mre11 and Rad50 comprise the catalytic component of the MRN complex with Rad50 involved in ATP hydrolysis and Mre11 functioning as a nuclease having both single-strand endonuclease and double-strand-specific 3'-5' exonuclease activity (1). The MRN complex is also involved in DNA damage signaling via activation of ATM kinase and in telomeres, where it may modulate t-loop formation (2). In addition, Mre11 is a component of the BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN) and interacts with DCLRE1C/Artemis as well as DCLRE1B/Apollo (3).

The MRN assembles as a hetero-hexamer consisting of 2 RAD50 subunits, 2 MRE11 nucleases, and 2 NBS1 subunits, specific to eukaryotes. Mre11 is highly conserved with the N-terminal region of the human protein sharing approximately 50% amino acid sequence identity with the yeast ortholog. Human Mre11 has 3 known isoforms with the predominant isoform having a theoretical molecular weight of 80.6kDa. This nuclear localized protein has high expression in proliferating tissues. Defects in Mre11 can lead to genomic instability, telomere shortening, aberrant meiosis, hypersensitivity to DNA damage, and ataxia telangiectasia-like disorder (ATLD). MRN expression has been associated with the prognosis of cancer and MRN mutants have been linked to cancer susceptibility in ovarian cancer and glioma (4, 5).

References

1. Paull TT. (2018) 20 Years of Mre11 Biology: No End in Sight. Mol Cell. 71(3):419-427. PMID: 30057197

2. Syed A, Tainer JA. (2018) The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair. Annu Rev Biochem. 87:263-294. PMID: 29709199

3. Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14(8):927-39. PMID: 10783165

4. Kim JH, Grosbart M, Anand R, Wyman C, Cejka P, Petrini JHJ. (2017) The Mre11-Nbs1 Interface Is Essential for Viability and Tumor Suppression. Cell Rep. 18(2):496-507. PMID: 28076792

5. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 18(1):169. PMID: 31767017

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mre11 Antibody (NBP2-59677JF646). (Showing 1 - 1 of 1 FAQ).

  1. Could you recommend an optimal Mre11 positive control for Western blot among your products?
    • NBL1-13219 would be the best product since it is intended for this purpose.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59677AF350
Alexa Fluor 405 NBP2-59677AF405
Alexa Fluor 488 NBP2-59677AF488
Alexa Fluor 532 NBP2-59677AF532
Alexa Fluor 594 NBP2-59677AF594
Alexa Fluor 647 NBP2-59677AF647
Alexa Fluor 700 NBP2-59677AF700
Alexa Fluor 750 NBP2-59677AF750
Allophycocyanin NBP2-59677APC
Biotin NBP2-59677B
DyLight 350 NBP2-59677UV
DyLight 405 NBP2-59677V
DyLight 488 NBP2-59677G
DyLight 550 NBP2-59677R
DyLight 594 NBP2-59677DL594
DyLight 650 NBP2-59677C
DyLight 680 NBP2-59677FR
DyLight 755 NBP2-59677IR
FITC NBP2-59677F
HRP NBP2-59677H
Janelia Fluor 549 NBP2-59677JF549
Janelia Fluor 646 NBP2-59677JF646
PE NBP2-59677PE
PerCP NBP2-59677PCP

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Bioinformatics Tool for Mre11 Antibody (NBP2-59677JF646)

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Blogs on Mre11.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Bioinformatics

Gene Symbol MRE11