Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit

Endothelial permeability in response to CLP. Plasma levels of endocan (A) and angiopoietin‐2 (B), soluble markers of endothelial permeability were measured in Sham and CLP rats (n = 8/group) via ELISA. *P < 0.05 versus Sham. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31054188), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat Angiopoietin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Angiopoietin-2 in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse Angiopoietin-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately.... Results obtained using natural Angiopoietin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural Angiopoietin-2.
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• Specificity: Natural and recombinant mouse and rat Angiopoietin-2.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Angpt2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Angiopoietin-2 (MANG20)(25)

Publications using MANG20

• Wang, L;Lin, B;Zhai, M;Hull, L;Cui, W;Xiao, M; Endothelial Dysfunction and Impaired Wound Healing Following Radiation Combined Skin Wound Injury International journal of molecular sciences 2024-11-21 [PMID: 39684207] (Mouse)
• Tian, R;Li, R;Chen, Y;Liu, D;Li, Y;He, S;Pan, T;Qu, H;Tan, R; Shenfu injection ameliorates endotoxemia-associated endothelial dysfunction and organ injury via inhibiting PI3K/Akt-mediated glycolysis Journal of ethnopharmacology 2024-07-30 [PMID: 39089657] (Mouse)
• Meder, L;Orschel, CI;Otto, CJ;Koker, M;Brägelmann, J;Ercanoglu, MS;Dähling, S;Compes, A;Selenz, C;Nill, M;Dietlein, F;Florin, A;Eich, ML;Borchmann, S;Odenthal, M;Blazquez, R;Hilberg, F;Klein, F;Hallek, M;Büttner, R;Reinhardt, HC;Ullrich, RT; Blocking the angiopoietin-2-dependent integrin ?-1 signaling axis abrogates small cell lung cancer invasion and metastasis JCI insight 2024-05-22 [PMID: 38775153] (Bioassay, Mouse)
• Allam, RM;El Kerdawy, AM;Gouda, AE;Ahmed, KA;Abdel-Mohsen, HT; Benzimidazole-oxindole hybrids as multi-kinase inhibitors targeting melanoma Bioorganic chemistry 2024-02-26 [PMID: 38457953] (Mouse)
• van Leeuwen, ALI;Beijer, E;Ibelings, R;Dekker, NAM;van der Steen, MRA;Roelofs, JJTH;van Meurs, M;Molema, G;van den Brom, CE; Female sex protects against renal edema, but not lung edema, in mice with partial deletion of the endothelial barrier regulator Tie2 compared to male sex PloS one 2023-11-16 [PMID: 37972011] (Mouse)
• Wideman, SK;Frost, JN;Richter, FC;Naylor, C;Lopes, JM;Viveiros, N;Teh, MR;Preston, AE;White, N;Yusuf, S;Draper, SJ;Armitage, AE;Duarte, TL;Drakesmith, H; Cellular iron governs the host response to malaria PLoS pathogens 2023-10-01 [PMID: 37812650] (Transgenic Mouse, Mouse)
• Lin, Y;McClennan, A;Hoffman, L; Characterization of the Ang/Tie2 Signaling Pathway in the Diaphragm Muscle of DMD Mice Biomedicines 2023-08-14 [PMID: 37626761] (Mouse)
• Atreya MR, Cvijanovich NZ, Fitzgerald JC et al. Detrimental effects of PCSK9 loss-of-function in the pediatric host response to sepsis are mediated through independent influence on Angiopoietin-1 Research square 2023-02-03 [PMID: 36778250] (Mouse)
• Patricia Himmels; Thi Thu Thao Nguyen; Maresa Caunt Mitzner; Alfonso Arrazate; Stacey Yeung; Jeremy Burton et al. T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interaction EMBO reports 2023-03-06 [PMID: 36621885] (Mouse, Transgenic Mouse)
• NM Karabid, T Wiedemann, S Gulde, H Mohr, RC Segaran, J Geppert, M Rohm, G Vitale, G Gaudenzi, A Dicitore, DP Ankerst, Y Chen, R Braren, G Kaissis, F Schilling, M Schillmaie, G Eisenhofer, S Herzig, F Roncaroli, JB Honegger, NS Pellegata Angpt2/Tie2 autostimulatory loop controls tumorigenesis Embo Molecular Medicine, 2022-03-10;0(0):e14364. 2022-03-10 [PMID: 35266635] (Rat)
• V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy EBioMedicine, 2021-11-07;73(0):103683. 2021-11-07 [PMID: 34758414] (Mouse)
• NV Bogatcheva, ME Coleman Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice Cells, 2021-03-24;10(4):. 2021-03-24 [PMID: 33804896] (Mouse)
• W Nahrendorf, A Ivens, PJ Spence Inducible mechanisms of disease tolerance provide an alternative strategy of acquired immunity to malaria Elife, 2021-03-23;10(0):. 2021-03-23 [PMID: 33752799] (Mouse)
• T Zhang, T Shibamoto, M Tanida, M Taniguchi, Y Kuda, W Yang, Y Kurata Angiopoietin-2 is released during anaphylactic hypotension in anesthetized and unanesthetized rats PLoS ONE, 2020-11-17;15(11):e0242026. 2020-11-17 [PMID: 33201925] (Rat)
• DC Cornelius, OK Travis, RW Tramel, M Borges-Rod, CH Baik, M Greer, CA Giachelli, GA Tardo, JM Williams NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture PLoS ONE, 2020-06-17;15(6):e0234039. 2020-06-17 [PMID: 32555710] (Rat)
• H Bae, KY Hong, CK Lee, C Jang, SJ Lee, K Choe, S Offermanns, Y He, HJ Lee, GY Koh Angiopoietin-2-integrin alpha5beta1 signaling enhances vascular fatty acid transport and prevents ectopic lipid-induced insulin resistance Nat Commun, 2020-06-12;11(1):2980. 2020-06-12 [PMID: 32532986] (Mouse)
• A Wolf, M Herb, M Schramm, T Langmann The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye Nat Commun, 2020-06-01;11(1):2709. 2020-06-01 [PMID: 32483169] (Mouse)
• Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS J. Clin. Invest., 2020-04-01;0(0):. 2020-04-01 [PMID: 32149735] (Mouse)
• J Zhang, S Li, S Zhang, Y Wang, S Jin, C Zhao, W Yang, Y Liu, G Kong Effect of Icariside II and Metformin on Penile Erectile Function, Histological Structure, Mitochondrial Autophagy, Glucose-Lipid Metabolism, Angiotensin II and Sex Hormone in Type 2 Diabetic Rats With Erectile Dysfunction Sex Med, 2020-03-05;0(0):. 2020-03-05 [PMID: 32147433] (Rat)
• AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861 Cancer Res., 2019-06-12;0(0):. 2019-06-12 [PMID: 31189647] (Mouse)
• DC Cornelius, CH Baik, OK Travis, DL White, CM Young, W Austin Pie, CA Shields, B Poudel, JM Williams NLRP3 inflammasome activation in platelets in response to sepsis Physiol Rep, 2019-05-01;7(9):e14073. 2019-05-01 [PMID: 31054188] (Rat)
• J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration Sci Adv, 2019-02-13;5(2):eaau6732. 2019-02-13 [PMID: 30788433] (Mouse)
• B Kim, C Jang, H Dharaneesw, J Li, M Bhide, S Yang, K Li, Z Arany Endothelial pyruvate kinase M2 maintains vascular integrity J. Clin. Invest., 2018-09-17;0(0):. 2018-09-17 [PMID: 30222136] (Mouse)
• VM Crowley, K Ayi, Z Lu, KT Liby, M Sporn, KC Kain Synthetic oleanane triterpenoids enhance blood brain barrier integrity and improve survival in experimental cerebral malaria Malar. J., 2017-11-14;16(1):463. 2017-11-14 [PMID: 29137631] (Mouse)
• Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med, 2016-08-22;22(9):1033-1042. 2016-08-22 [PMID: 27548575] (Mouse)

Bioinformatics

• Gene Symbol: Angpt2