Lipocalin-2/NGAL Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by ...read more
Lipocalin‑2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for ...read more
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Lipocalin-2/NGAL Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL
Gln21-Asn200
Accession # P11672
Specificity
Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat Lipocalin-2/NGAL is observed and less than 10% cross-reactivity with recombinant human (rh) Lipocalin-2/NGAL is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LCN2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF1857 in the following applications:

Publications
Read Publications using
AF1857 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody [Unconjugated]

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin

Background

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3- 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).
  1. Hraba-Renevey, S. et al. (1989) Oncogene. 4:601. 
  2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipocalin-2/NGAL Antibody (AF1857)(35)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Bioassay, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
Bioassay
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(2)
Western Blot
(29)
All Applications
Filter By Species
Mouse
(34)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using AF1857 Applications Species
KJ Meade, F Sanchez, A Aguayo, N Nadales, SG Hamalian, TL Uhlendorf, LR Banner, JA Kelber Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung Oncotarget, 2019;10(32):3027-3039. 2019 [PMID: 31105883] (Western Blot, Mouse) Western Blot Mouse
K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee Negr1 controls adult hippocampal neurogenesis and affective behaviors Mol. Psychiatry, 2019;0(0):. 2019 [PMID: 30651602] (Western Blot, Mouse) Western Blot Mouse
TA Yakulov, AP Todkar, K Slanchev, J Wiegel, A Bona, M Gro beta, A Scholz, I Hess, A Wurditsch, F Grahammer, TB Huber, V Lecaudey, T Bork, J Hochrein, M Boerries, J Leenders, P de Tullio, F Jouret, A Kramer-Zuc, G Walz CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury Nat Commun, 2018;9(1):3660. 2018 [PMID: 30202007] (Western Blot, Mouse) Western Blot Mouse
H Langhorst, R Jüttner, D Groneberg, A Mohtashamd, L Pelz, B Purfürst, KM Schmidt-Ot, A Friebe, FG Rathjen The IgCAM CLMP is required for intestinal and ureteral smooth muscle contraction by regulating Connexin43 and 45 expression in mice Dis Model Mech, 2018;0(0):. 2018 [PMID: 29361518] (Western Blot, Mouse) Western Blot Mouse
S Ueno, A Yoneshige, Y Koriyama, M Hagiyama, Y Shimomura, A Ito Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice Invest. Ophthalmol. Vis. Sci., 2018;59(1):370-380. 2018 [PMID: 29346801] (Western Blot, Mouse) Western Blot Mouse
Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease JCI Insight, 2017;2(23):. 2017 [PMID: 29212948] (Western Blot, Mouse) Western Blot Mouse
M Trudu, C Schaeffer, M Riba, M Ikehata, P Brambilla, P Messa, F Martinelli, MP Rastaldi, L Rampoldi Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations Sci Rep, 2017;7(1):7383. 2017 [PMID: 28785050] (Western Blot, Mouse) Western Blot Mouse
A Eskilsson, T Matsuwaki, K Shionoya, E Mirrasekhi, J Zajdel, M Schwaninge, D Engblom, A Blomqvist Immune-induced fever is dependent on local but not generalized prostaglandin E2 synthesis in the brain J. Neurosci., 2017;0(0):. 2017 [PMID: 28438967] (IHC, Mouse) IHC Mouse
I Mosialou, S Shikhel, JM Liu, A Maurizi, N Luo, Z He, Y Huang, H Zong, RA Friedman, J Barasch, P Lanzano, L Deng, RL Leibel, M Rubin, T Nicholas, W Chung, LM Zeltser, KW Williams, JE Pessin, S Kousteni MC4R-dependent suppression of appetite by bone-derived lipocalin 2 Nature, 2017;543(7645):385-390. 2017 [PMID: 28273060] (Western Blot, Mouse) Western Blot Mouse
Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms PLoS ONE, 2016;11(11):e0166153. 2016 [PMID: 27861574] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 35 Publications.

Reviews for Lipocalin-2/NGAL Antibody (AF1857) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1857:
Filter by Applications
IHC
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Lipocalin-2/NGAL AF1857
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reviewed by:
Anonymous
IHC Mouse 05/03/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedskin tumor
SpeciesMouse
Immunocytochemistry Lipocalin-2/NGAL AF1857
Enlarge
reviewed by:
Qi Miao
ICC Mouse 05/03/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse skin,Mouse Keratinocytes
SpeciesMouse

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Secondary Antibodies

 

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Biotin BAF1857

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Recent Reviews

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2
4
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2
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1
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Anonymous
05/03/2017
Application: IHC
Species: Mouse

Qi Miao
05/03/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol LCN2
Uniprot