Lipocalin-2/NGAL Antibody [Unconjugated]

Lipocalin‑2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected forLipocalin‑2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-MouseLipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipocalin-2/NGAL Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL
  Gln21-Asn200
  Accession # P11672
• Specificity: Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LCN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody [Unconjugated]

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3- 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (K_d = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_d values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

1. Hraba-Renevey, S. et al. (1989) Oncogene. 4:601. 
2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
4. Devireddy, L.R. et al. (2001) Science 293:829.
5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
7. Flo, T.H. et al. (2004) Nature 432:917.
8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (AF1857)(56)

Publications using AF1857

• R Greite, J Störmer, F Gueler, R Khalikov, A Haverich, C Kühn, N Madrahimov, R Natanov Different Acute Kidney Injury Patterns after Renal Ischemia Reperfusion Injury and Extracorporeal Membrane Oxygenation in Mice International Journal of Molecular Sciences, 2022;23(19):. 2022 [PMID: 36232304] (IHC, Mouse)
• C Zhang, Y Guan, J Zou, X Yang, G Bayliss, S Zhuang Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury Cell Death & Disease, 2022;13(9):770. 2022 [PMID: 36068197] (IHC, Mouse)
• JR Sheldon, LE Himmel, DE Kunkle, AJ Monteith, KN Maloney, EP Skaar Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection PloS Pathogens, 2022;18(9):e1010809. 2022 [PMID: 36054235] (Western Blot, Mouse)
• M Silva Oliv, J Schira-Hei, L Reiche, S Han, VCM de Amorim, I Lewen, J Gruchot, P Göttle, R Akkermann, K Azim, P Küry Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations EBioMedicine, 2022;83(0):104204. 2022 [PMID: 35952494] (IHC, Mouse)
• HJ Shin, Z Jin, HS An, G Park, JY Lee, SJ Lee, HM Jang, EA Jeong, KE Kim, J Lee, DY Yoo, GS Roh Lipocalin-2 Deficiency Reduces Hepatic and Hippocampal Triggering Receptor Expressed on Myeloid Cells-2 Expressions in High-Fat Diet/Streptozotocin-Induced Diabetic Mice Brain sciences, 2022;12(7):. 2022 [PMID: 35884685] (Western Blot, Mouse)
• X Xiang, X Tang, Y Yu, S Xie, L Liu, M Chen, R Zhang, X Kang, Y Zheng, G Yang, S Gan, S Zhu Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia Journal of Neuroinflammation, 2022;19(1):92. 2022 [PMID: 35413913] (IHC, Mouse)
• T Eckes, S Patyna, A Koch, A Oftring, S Gauer, N Obermüller, S Schwalm, L Schaefer, J Chun, HJ Gröne, J Pfeilschif Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy International Journal of Molecular Sciences, 2022;23(7):. 2022 [PMID: 35409312] (Western Blot, Mouse)
• T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice Nature Communications, 2022;13(1):1134. 2022 [PMID: 35241660] (ICC, Mouse)
• TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury JCI Insight, 2022;0(0):. 2022 [PMID: 35230973] (Western Blot, Mouse)
• N Helmrich, M Roderfeld, A Baier, A Windhorst, D Herebian, E Mayatepek, C Dierkes, M Ocker, D Glebe, B Christ, Y Churin, K Irungbam, E Roeb Pharmacological Antagonization of Cannabinoid Receptor 1 Improves Cholestasis in Abcb4-/- Mice Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 34954190] (Western Blot, Mouse)
• F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 2021;12(1):7333. 2021 [PMID: 34921145] (IHC, Mouse)
• N Ma, Z Wei, J Hu, W Gu, X Ci Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway Frontiers in Pharmacology, 2021;12(0):768700. 2021 [PMID: 34858188] (Western Blot, Mouse)
• S Talley, R Valiauga, L Anderson, AR Cannon, MA Choudhry, EM Campbell DSS-induced inflammation in the colon drives a proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod Journal of Neuroinflammation, 2021;18(1):263. 2021 [PMID: 34758843] (Western Blot, Mouse)
• H Ahn, G Lee, J Kim, J Park, SG Kang, SI Yoon, E Lee, GS Lee NLRP3 Triggers Attenuate Lipocalin-2 Expression Independent with Inflammasome Activation Cells, 2021;10(7):. 2021 [PMID: 34359830] (Western Blot, Mouse)
• B Olson, X Zhu, MA Norgard, P Diba, PR Levasseur, AC Buenafe, C Huisman, KG Burfeind, KA Michaelis, G Kong, T Braun, DL Marks Chronic cerebral lipocalin 2 exposure elicits hippocampal neuronal dysfunction and cognitive impairment Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 34245812] (IHC, Mouse)
• B Olson, X Zhu, MA Norgard, PR Levasseur, JT Butler, A Buenafe, KG Burfeind, KA Michaelis, KR Pelz, H Mendez, J Edwards, SM Krasnow, AJ Grossberg, DL Marks Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia Nature Communications, 2021;12(1):2057. 2021 [PMID: 33824339] (IHC, Mouse)
• E Staurenghi, V Cerrato, P Gamba, G Testa, S Giannelli, V Leoni, C Caccia, A Buffo, W Noble, BG Perez-Niev, G Leonarduzz Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2 Redox Biology, 2020;39(0):101837. 2020 [PMID: 33360775] (Western Blot, Mouse)
• A Mondal, D Bose, P Saha, S Sarkar, R Seth, D Kimono, M Albadrani, M Nagarkatti, P Nagarkatti, S Chatterjee Lipocalin 2 induces neuroinflammation and blood-brain barrier dysfunction through liver-brain axis in murine model of nonalcoholic steatohepatitis J Neuroinflammation, 2020;17(1):201. 2020 [PMID: 32622362] (IHC, Mouse)
• EM Buhl, S Djudjaj, BM Klinkhamme, K Ermert, VG Puelles, MT Lindenmeye, CD Cohen, C He, E Borkham-Ka, R Weiskirche, B Denecke, P Trairatphi, J Saez-Rodri, TB Huber, LE Olson, J Floege, P Boor Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis EMBO Mol Med, 2020;0(0):e11021. 2020 [PMID: 31943786] (IHC, Mouse)
• K Chella Kri, S Sabir, M Shum, Y Meng, R Acín-Pérez, JM Lang, RR Floyd, L Vergnes, MM Seldin, BK Fuqua, DW Jayasekera, SK Nand, DC Anum, C Pan, L Stiles, M Péterfy, K Reue, M Liesa, AJ Lusis Sex-specific metabolic functions of adipose Lipocalin-2 Mol Metab, 2019;30(0):30-47. 2019 [PMID: 31767179] (Western Blot, Mouse)
• L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking Cell Rep, 2019;29(7):2067-2077.e6. 2019 [PMID: 31722218] (IP, Human)
• KJ Meade, F Sanchez, A Aguayo, N Nadales, SG Hamalian, TL Uhlendorf, LR Banner, JA Kelber Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung Oncotarget, 2019;10(32):3027-3039. 2019 [PMID: 31105883] (Western Blot, Mouse)
• K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee Negr1 controls adult hippocampal neurogenesis and affective behaviors Mol. Psychiatry, 2019;0(0):. 2019 [PMID: 30651602] (Western Blot, Mouse)
• TA Yakulov, AP Todkar, K Slanchev, J Wiegel, A Bona, M Gro beta , A Scholz, I Hess, A Wurditsch, F Grahammer, TB Huber, V Lecaudey, T Bork, J Hochrein, M Boerries, J Leenders, P de Tullio, F Jouret, A Kramer-Zuc, G Walz CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury Nat Commun, 2018;9(1):3660. 2018 [PMID: 30202007] (Western Blot, Mouse)
• H Langhorst, R Jüttner, D Groneberg, A Mohtashamd, L Pelz, B Purfürst, KM Schmidt-Ot, A Friebe, FG Rathjen The IgCAM CLMP is required for intestinal and ureteral smooth muscle contraction by regulating Connexin43 and 45 expression in mice Dis Model Mech, 2018;0(0):. 2018 [PMID: 29361518] (Western Blot, Mouse)
• S Ueno, A Yoneshige, Y Koriyama, M Hagiyama, Y Shimomura, A Ito Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice Invest. Ophthalmol. Vis. Sci., 2018;59(1):370-380. 2018 [PMID: 29346801] (Western Blot, Mouse)
• Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease JCI Insight, 2017;2(23):. 2017 [PMID: 29212948] (Western Blot, Mouse)
• M Trudu, C Schaeffer, M Riba, M Ikehata, P Brambilla, P Messa, F Martinelli, MP Rastaldi, L Rampoldi Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations Sci Rep, 2017;7(1):7383. 2017 [PMID: 28785050] (Western Blot, Mouse)
• A Eskilsson, T Matsuwaki, K Shionoya, E Mirrasekhi, J Zajdel, M Schwaninge, D Engblom, A Blomqvist Immune-induced fever is dependent on local but not generalized prostaglandin E2 synthesis in the brain J. Neurosci., 2017;0(0):. 2017 [PMID: 28438967] (IHC, Mouse)
• I Mosialou, S Shikhel, JM Liu, A Maurizi, N Luo, Z He, Y Huang, H Zong, RA Friedman, J Barasch, P Lanzano, L Deng, RL Leibel, M Rubin, T Nicholas, W Chung, LM Zeltser, KW Williams, JE Pessin, S Kousteni MC4R-dependent suppression of appetite by bone-derived lipocalin 2 Nature, 2017;543(7645):385-390. 2017 [PMID: 28273060] (Western Blot, Mouse)
• Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms PLoS ONE, 2016;11(11):e0166153. 2016 [PMID: 27861574] (IHC-Fr, Mouse)
• Keap1 hypomorphism protects against ischemic and obstructive kidney disease Sci Rep, 2016;6(0):36185. 2016 [PMID: 27804998] (Western Blot, Mouse)
• Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 2016;0(0):. 2016 [PMID: 27382022] (Western Blot, Mouse)
• Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons PLoS Pathog, 2016;12(5):e1005572. 2016 [PMID: 27149619] (Western Blot, Mouse)
• GJ Song, M Jung, JH Kim, H Park, MH Rahman, S Zhang, ZY Zhang, DH Park, H Kook, IK Lee, K Suk A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation J Neuroinflammation, 2016;13(1):86. 2016 [PMID: 27095436] (Western Blot, Mouse)
• Weng Y, Wang G, Messing R, Chou W Identification of lipocalin-2 as a PKCdelta phosphorylation substrate in neutrophils. J Biomed Sci, 2015;22(0):21. 2015 [PMID: 25890235] (Western Blot, Mouse)
• Drew B, Hamidi H, Zhou Z, Villanueva C, Krum S, Calkin A, Parks B, Ribas V, Kalajian N, Phun J, Daraei P, Christofk H, Hewitt S, Korach K, Tontonoz P, Lusis A, Slamon D, Hurvitz S, Hevener A Estrogen receptor (ER)alpha-regulated lipocalin 2 expression in adipose tissue links obesity with breast cancer progression. J Biol Chem, 2015;290(9):5566-81. 2015 [PMID: 25468909] (Western Blot, Mouse)
• Guo H, Jin D, Chen X Lipocalin 2 is a regulator of macrophage polarization and NF-kappaB/STAT3 pathway activation. Mol Endocrinol, 2014;28(10):1616-28. 2014 [PMID: 25127375] (Western Blot, Mouse)
• Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis. J Bone Miner Res, 2015;30(2):357-68. 2015 [PMID: 25112732] (Bioassay, Mouse)
• Grahammer F, Haenisch N, Steinhardt F, Sander L, Roerden M, Arnold F, Cordts T, Wanner N, Reichardt W, Kerjaschki D, Ruegg M, Hall M, Moulin P, Busch H, Boerries M, Walz G, Artunc F, Huber T mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress. Proc Natl Acad Sci U S A, 2014;111(27):E2817-26. 2014 [PMID: 24958889] (Western Blot, Mouse)
• Paragas N, Kulkarni R, Werth M, Schmidt-Ott K, Forster C, Deng R, Zhang Q, Singer E, Klose A, Shen T, Francis K, Ray S, Vijayakumar S, Seward S, Bovino M, Xu K, Takabe Y, Amaral F, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin C, Qiu A, Al-Awqati Q, Ratner A, Barasch J alpha-Intercalated cells defend the urinary system from bacterial infection. J Clin Invest, 2014;124(7):2963-76. 2014 [PMID: 24937428] (Western Blot, Mouse)
• Zhang Y, Guo H, Deis J, Mashek M, Zhao M, Ariyakumar D, Armien A, Bernlohr D, Mashek D, Chen X Lipocalin 2 regulates brown fat activation via a nonadrenergic activation mechanism. J Biol Chem, 2014;289(32):22063-77. 2014 [PMID: 24917675] (Western Blot, Mouse)
• Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2. Nat Commun, 2014;5(0):4088. 2014 [PMID: 24909826]
• Glenthoj A, Dahl S, Larsen M, Cowland J, Borregaard N Human alpha-defensin expression is not dependent on CCAAT/enhancer binding protein-epsilon in a murine model. PLoS ONE, 2014;9(3):e92471. 2014 [PMID: 24658030] (Western Blot, Mouse)
• Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity, 2014;40(2):262-73. 2014 [PMID: 24508234] (Western Blot, Mouse)
• Nocon A, Ip J, Terry R, Lim S, Getts D, Muller M, Hofer M, King N, Campbell I The bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west Nile virus encephalitis. J Virol, 2014;88(1):679-89. 2014 [PMID: 24173226] (Western Blot, Mouse)
• Tasso , Roberta, Ulivi , Valentin, Reverberi , Daniele, Lo Sicco , Claudia, Descalzi , Fiorella, Cancedda , Ranieri In vivo implanted bone marrow-derived mesenchymal stem cells trigger a cascade of cellular events leading to the formation of an ectopic bone regenerative niche. Stem Cells Dev, 2013;22(24):3178-91. 2013 [PMID: 23924051] (Western Blot, Mouse)
• Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012;7(0):87-104. 2012 [PMID: 22837640] (Western Blot, Mouse)
• Cramer EP, Glenthoj A, Hager M, Juncker-Jensen A, Engelholm LH, Santoni-Rugiu E, Lund LR, Laerum OD, Cowland JB, Borregaard N No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer. PLoS ONE, 2012;7(6):e39646. 2012 [PMID: 22737251] (Western Blot, Mouse)
• Malhotra D, Fletcher AL, Astarita J, Lukacs-Kornek V, Tayalia P, Gonzalez SF, Elpek KG, Chang SK, Knoblich K, Hemler ME, Brenner MB, Carroll MC, Mooney DJ, Turley SJ, Zhou Y, Shinton SA, Hardy RR, Bezman NA, Sun JC, Kim CC, Lanier LL, Miller J, Brown B, Merad M, Fletcher AL, Elpek KG, Bellemare-Pelletier A, Malhotra D, Turley SJ, Narayan K, Sylvia K, Kang J, Gazit R, Garrison B, Rossi DJ, Jojic V, Koller D, Jianu R, Laidlaw D, Costello J, Collins J, Cohen N, Brennan P, Brenner MB, Shay T, Regev A, Francis Kim TN, Rao A, Wagers EL, Gautier C, Jakubzick GJ, Randolph P, Monach AJ, Best J, Knell A, Goldrath T, Heng T, Kreslavsky M, Painter D, Mathis C, Benoist Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks. Nat. Immunol., 2012;13(5):499-510. 2012 [PMID: 22466668] (ICC, Mouse)
• Ip JP, Nocon AL, Hofer MJ, Lim SL, Muller M, Campbell IL Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration. J Neuroinflammation, 2011;8(0):124. 2011 [PMID: 21943033] (Western Blot, Mouse)
• Mahadevan NR, Rodvold J, Almanza G, Perez AF, Wheeler MC, Zanetti M ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-kappaB-dependent manner. BMC Cancer, 2011;11(0):229. 2011 [PMID: 21649922] (Western Blot, Mouse)
• Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons. Kidney Int., 2009;75(3):285-94. 2009 [PMID: 19148153] (Western Blot, Mouse)
• Lee S, Park JY, Lee WH, Kim H, Park HC, Mori K, Suk K Lipocalin-2 is an autocrine mediator of reactive astrocytosis. J. Neurosci., 2009;29(1):234-49. 2009 [PMID: 19129400] (Western Blot, Mouse)
• Yan QW, Yang Q, Mody N, Graham TE, Hsu CH, Xu Z, Houstis NE, Kahn BB, Rosen ED The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance. Diabetes, 2007;56(10):2533-40. 2007 [PMID: 17639021] (Western Blot, Mouse)
• Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung. Eur. Respir. J., 2006;28(2):275-85. 2006 [PMID: 16641123] (IHC-Fr, Mouse)

Reviews for Lipocalin-2/NGAL Antibody (AF1857) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Qi Miao IHC Mouse 05/03/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: skin tumor
• Species: Mouse

reviewed by: Verified Customer ICC Mouse 05/03/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Keratinocytes,mouse skin
• Species: Mouse

Bioinformatics

• Gene Symbol: LCN2
• Uniprot:
  • P11672()