Mouse IL-17A/F Heterodimer Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-17A/F Heterodimer Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse IL-17A/F Heterodimer immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-17A/F heterodimer in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-17A/F heterodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL...-17A/F heterodimer. Results obtained using natural mouse IL-17A/F heterodimer showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17A/F heterodimer.
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• Specificity: Natural and recombinant mouse IL-17A/F heterodimer
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-17A/F Heterodimer Quantikine ELISA Kit

• CTLA8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• Cytotoxic T-Lymphocyte-Associated Protein 8
• IL17
• IL-17
• IL-17A
• IL-17A/F Heterodimer
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A
• interleukin-17A

Background

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-17A/F Heterodimer (M17AF0)(6)

Publications using M17AF0

• AD Friedrich, J Leoni, ML Paz, DH González M Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG Modulates Dendritic Cells and T Cells in the Gut Nutrients, 2022-02-08;14(3):. 2022-02-08 [PMID: 35277082] (Mouse)
• X Song, H Wen, L Zuo, Z Geng, J Nian, L Wang, Y Jiang, J Tao, Z Zhu, X Wu, Z Wang, X Zhang, L Yu, H Zhao, P Xiang, J Li, L Shen, J Hu Epac-2 ameliorates spontaneous colitis in Il-10-/- mice by protecting the intestinal barrier and suppressing NF-kappaB/MAPK signalling Journal of Cellular and Molecular Medicine, 2021-12-03;0(0):. 2021-12-03 [PMID: 34862717] (Mouse)
• L Lu, Y Wang, L Zhou, Y Li, X Zhang, X Hu, S Shi, W He V&gamma4 T cell-derived IL-17A is essential for amplification of inflammatory cascades in ischemic brain tissue after stroke International immunopharmacology, 2021-05-24;96(0):107678. 2021-05-24 [PMID: 34162129] (Mouse)
• J Zhou, N Zhang, W Zhang, C Lu, F Xu The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation Cell & bioscience, 2021-05-12;11(1):84. 2021-05-12 [PMID: 33980319] (Mouse)
• J Xuan, A Huang, D Hu, J Geng, Y Tian, Z Cheng, Y Qiu Huagan tongluo Fang improves liver fibrosis via down-regulating miR-184 and up-regulating FOXO1 to inhibit Th17 cell differentiation Exp. Mol. Pathol., 2020-05-04;115(0):104447. 2020-05-04 [PMID: 32380055] (Mouse)
• Qin S, Wen J, Bai X, Chen T, Zheng R, Zhou G, Ma J, Feng J, Zhong B, Li Y Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis. Mol Med Rep, 2014-04-09;9(6):2097-104. 2014-04-09 [PMID: 24718773] (Mouse)

FAQs for IL-17A/F Heterodimer (M17AF0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.