Mouse ICAM-1/CD54 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse ICAM-1/CD54 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Icam1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse ICAM-1/CD54 DuoSet ELISA, 15 Plate

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.

Publications for ICAM-1/CD54 (DY796)(10)

Publications using DY796

• Vangrieken, P;Scheijen, JLJM;Schiffers, PMH;van de Waarenburg, MPH;Foulquier, S;Schalkwijk, CCG; Modelling the effects of elevated methylglyoxal levels on vascular and metabolic complications Scientific reports 2025-02-19 [PMID: 39972072] (Mouse)
• Manandhar, S;Gaddam, RR;Chambers, S;Bhatia, M; Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis Biomolecules 2024-01-09 [PMID: 38254684] (Mouse)
• Manandhar, S;Chambers, S;Miller, A;Ishii, I;Bhatia, M; Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells International journal of molecular sciences 2023-09-04 [PMID: 37686458] (Mouse, Transgenic Mouse)
• F Seidel, R Kleemann, W van Duyven, N van Trigt, N Keijzer, S van der Ko, C van Kooten, L Verschuren, A Menke, AJ Kiliaan, J Winter, TR Hughes, BP Morgan, F Baas, K Fluiter, MC Morrison Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice International Journal of Molecular Sciences, 2022-09-14;23(18):. 2022-09-14 [PMID: 36142647] (Mouse)
• L Huang, W Liang, K Zhou, RA Wassel, ZD Ridge, JX Ma, B Wang Therapeutic Effects of Fenofibrate Nano-Emulsion Eye Drops on Retinal Vascular Leakage and Neovascularization Biology, 2021-12-15;10(12):. 2021-12-15 [PMID: 34943243] (Mouse)
• G Cohen, P Chandran, RM Lorsung, LE Tomlinson, M Sundby, SR Burks, JA Frank The Impact of Focused Ultrasound in Two Tumor Models: Temporal Alterations in the Natural History on Tumor Microenvironment and Immune Cell Response Cancers (Basel), 2020-02-04;12(2):. 2020-02-04 [PMID: 32033171] (Mouse)
• A Leiva, S Contreras-, L Amigo, E Sepúlveda, M Boric, V Quiñones, D Busso, A Rigotti Gugulipid causes hypercholesterolemia leading to endothelial dysfunction, increased atherosclerosis, and premature death by ischemic heart disease in male mice PLoS ONE, 2017-09-14;12(9):e0184280. 2017-09-14 [PMID: 28910310] (Mouse)
• MJ Haley, G Mullard, KA Hollywood, GJ Cooper, WB Dunn, CB Lawrence Adipose tissue, metabolic and inflammatory responses to stroke are altered in obese mice Dis Model Mech, 2017-08-10;0(0):. 2017-08-10 [PMID: 28798136] (Mouse)
• LN Masi, AR Martins, AR Crisma, CL do Amaral, MR Davanso, TDA Serdan, RDC da Cunha d, MM Cruz, MIC Alonso-Val, RP Torres, J Mancini-Fi, JNB Pereira, MM da Silva R, EA Liberti, SM Hirabara, R Curi Combination of a high-fat diet with sweetened condensed milk exacerbates inflammation and insulin resistance induced by each separately in mice Sci Rep, 2017-06-21;7(1):3937. 2017-06-21 [PMID: 28638152] (Mouse)
• Hegde A, Zhang H, Moochhala SM, Bhatia M Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis. J. Leukoc. Biol., 2007-06-12;82(3):678-85. 2007-06-12 [PMID: 17565047] (Mouse)

FAQs for ICAM-1/CD54 (DY796). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Icam1