Mouse CXCL12/SDF-1 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse CXCL12/SDF-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Cxcl12

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CXCL12/SDF-1 DuoSet ELISA, 15 Plate

• chemokine (C-X-C motif) ligand 12
• C-X-C motif chemokine 12
• CXCL12
• hIRH
• hSDF-1
• Intercrine reduced in hepatomas
• IRH
• PBSF
• PBSFSDF-1a
• Pre-B cell growth-stimulating factor
• SCYB12
• SDF1
• SDF-1
• SDF1A
• SDF1B
• SDF1SDF-1b
• stromal cell-derived factor 1
• TLSF
• TLSF-a
• TLSF-b
• TPAR1

Background

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Publications for CXCL12/SDF-1 (DY460)(28)

Publications using DY460

• Agueda-Oyarzabal, M;Isidor, MS;Pluci?ska, K;Ingerslev, LR;Dmytriyeva, O;Petersen, PSS;Laftih, S;Pontoppidan, AB;Henningsen, JB;Rupar, K;Brown, EL;Schwartz, TW;Barrès, R;Gerhart-Hines, Z;Schéele, CC;Emanuelli, B; Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine Molecular metabolism 2025-01-21 [PMID: 39848402] (Mouse)
• Morgaenko, K;Arneja, A;Ball, AG;Putelo, AM;Munson, JM;Rutkowski, MR;Pompano, RR; Ex vivo model of breast cancer cell invasion in live lymph node tissue bioRxiv : the preprint server for biology 2024-07-22 [PMID: 39091774] (Mouse)
• Colella, P;Sayana, R;Suarez-Nieto, MV;Sarno, J;Nyame, K;Xiong, J;Pimentel Vera, LN;Arozqueta Basurto, J;Corbo, M;Limaye, A;Davis, KL;Abu-Remaileh, M;Gomez-Ospina, N; CNS-wide repopulation by hematopoietic-derived microglia-like cells corrects progranulin deficiency in mice Nature communications 2024-07-05 [PMID: 38969669] (Mouse)
• L Spagnuolo, V Puddinu, N Boss, T Spinetti, A Oberson, J Widmer, I Mottas, C Hotz, ME Bianchi, M Uguccioni, C Bourquin HMGB1 promotes CXCL12-dependent egress of murine B cells from Peyer's patches in homeostasis European Journal of Immunology, 2021-06-16;0(0):. 2021-06-16 [PMID: 34060652] (Mouse)
• A Di Pardo, E Ciaglia, M Cattaneo, A Maciag, F Montella, V Lopardo, A Ferrario, F Villa, M Madonna, E Amico, A Carrizzo, A Damato, G Pepe, F Marracino, A Auricchio, C Vecchione, V Maglione, AA Puca The longevity-associated variant of BPIFB4 improves a CXCR4-mediated striatum-microglia crosstalk preventing disease progression in a mouse model of Huntington's disease Cell Death Dis, 2020-07-18;11(7):546. 2020-07-18 [PMID: 32683420] (Mouse)
• LE Hand, KJ Gray, SH Dickson, DA Simpkins, DW Ray, JE Konkel, MR Hepworth, JE Gibbs Regulatory T cells confer a circadian signature on inflammatory arthritis Nat Commun, 2020-04-03;11(1):1658. 2020-04-03 [PMID: 32245954] (Mouse)
• M Xu, C Ge, Y Qin, D Lou, Q Li, J Feng, Y Wu, L Hu, B Wang, J Tan Functional loss of inactive rhomboid-like protein�2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation Mol Metab, 2020-01-22;34(0):112-123. 2020-01-22 [PMID: 32180551] (Mouse)
• BJ Meijer, FP Giugliano, B Baan, JHM van der Me, S Meisner, M van Roest, PJ Koelink, RJ de Boer, N Jones, W Breitwiese, NN van der We, ME Wildenberg, GR van den Br, J Heijmans, V Muncan ATF2 and ATF7 Are Critical Mediators of Intestinal Epithelial Repair Cell Mol Gastroenterol Hepatol, 2020-01-17;0(0):. 2020-01-17 [PMID: 31958521] (Mouse)
• LN Zamproni, D Teixeira, A Arnaut All, I Longo Maug, A des Rieux, MA Porcionatt Decreased viability and neurite length in neural cells treated with chitosan-dextran sulfate nanocomplexes Neurotoxicology, 2019-10-09;76(0):33-43. 2019-10-09 [PMID: 31605718] (Mouse)
• FH Brennan, T Jogia, ER Gillespie, LV Blomster, XX Li, B Nowlan, GM Williams, E Jacobson, GW Osborne, FA Meunier, SM Taylor, KE Campbell, KP MacDonald, JP Levesque, TM Woodruff, MJ Ruitenberg Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2 JCI Insight, 2019-05-02;4(9):. 2019-05-02 [PMID: 31045582] (Mouse)
• S Avraham, B Korin, S Aviram, D Shechter, Y Shaked, A Aronheim ATF3 and JDP2 deficiency in cancer associated fibroblasts promotes tumor growth via SDF-1 transcription Oncogene, 2019-01-22;0(0):. 2019-01-22 [PMID: 30670778] (Mouse)
• B Wang, Y Guo, X Chen, C Zeng, Q Hu, W Yin, W Li, H Xie, B Zhang, X Huang, F Yu Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2 Int J Nanomedicine, 2018-11-12;13(0):7395-7408. 2018-11-12 [PMID: 30519022] (Human)
• K Bröker, J Figge, AF Magnusen, RA Manz, J Köhl, CM Karsten A Novel Role for C5a in B-1 Cell Homeostasis Front Immunol, 2018-02-19;9(0):258. 2018-02-19 [PMID: 29520270] (Mouse)
• W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection Cytokine, 2018-02-01;0(0):. 2018-02-01 [PMID: 29396045] (Human)
• BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response Cell Mol Gastroenterol Hepatol, 2017-09-05;5(1):67-82.e1. 2017-09-05 [PMID: 29276753] (Mouse)
• P Marona, J Górka, Z Mazurek, W Wilk, J Rys, M Majka, J Jura, K Miekus MCPIP1 downregulation in clear cell renal cell carcinoma promotes vascularization and metastatic progression Cancer Res., 2017-07-17;0(0):. 2017-07-17 [PMID: 28716897] (Mouse)
• B Isaacson, T Hadad, A Glasner, C Gur, Z Granot, G Bachrach, O Mandelboim Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection Cell Rep, 2017-07-05;20(1):40-47. 2017-07-05 [PMID: 28683322] (Mouse)
• M Losada-Bar, A Umaña-Pére, S Cuervo-Esc, LR Berbert, R Porrozzi, FN Morgado, DA Mendes-da-, W Savino, M Sánchez-Gó, P Cuervo Protein malnutrition promotes dysregulation of molecules involved in T cell migration in the thymus of mice infected with Leishmania infantum Sci Rep, 2017-04-11;7(0):45991. 2017-04-11 [PMID: 28397794] (Mouse)
• SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia Sci Rep, 2016-10-07;6(0):34416. 2016-10-07 [PMID: 27713493] (Human)
• The time-of-day of myocardial infarction onset affects healing through oscillations in cardiac neutrophil recruitment EMBO Mol Med, 2016-08-01;0(0):. 2016-08-01 [PMID: 27226028] (Mouse)
• Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy. Nat Immunol, 2015-06-15;16(8):850-8. 2015-06-15 [PMID: 26075911] (Mouse)
• Projahn D, Simsekyilmaz S, Singh S, Kanzler I, Kramp B, Langer M, Burlacu A, Bernhagen J, Klee D, Zernecke A, Hackeng T, Groll J, Weber C, Liehn E, Koenen R Controlled intramyocardial release of engineered chemokines by biodegradable hydrogels as a treatment approach of myocardial infarction. J Cell Mol Med, 2014-02-06;18(5):790-800. 2014-02-06 [PMID: 24512349] (Mouse)
• Przybyla B, Shafirstein G, Vishal S, Dennis R, Griffin R Molecular changes in bone marrow, tumor and serum after conductive ablation of murine 4T1 breast carcinoma. Int J Oncol, 2013-11-21;44(2):600-8. 2013-11-21 [PMID: 24270800] (Mouse)
• Ponzetta A, Sciume G, Benigni G, Antonangeli F, Morrone S, Santoni A, Bernardini G CX3CR1 regulates the maintenance of KLRG1+ NK cells into the bone marrow by promoting their entry into circulation. J Immunol, 2013-11-01;191(11):5684-94. 2013-11-01 [PMID: 24184559] (Mouse)
• Kevin Dhaliwal, Emma Scholefield, David Ferenbach, Michael Gibbons, Rodger Duffin, David A. Dorward et al. Monocytes Control Second-Phase Neutrophil Emigration in Established Lipopolysaccharide-induced Murine Lung Injury American Journal of Respiratory and Critical Care Medicine 2012-09-15 [PMID: 22822022] (Mouse)
• Schiraldi M, Raucci A, Munoz LM, Livoti E, Celona B, Venereau E, Apuzzo T, De Marchis F, Pedotti M, Bachi A, Thelen M, Varani L, Mellado M, Proudfoot A, Bianchi ME, Uguccioni M HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4. J. Exp. Med., 2012-02-27;209(3):551-63. 2012-02-27 [PMID: 22370717] (Mouse)
• Hughes DJ, Kipar A, Leeming GH, Bennett E, Howarth D, Cummerson JA, Papoula-Pereira R, Flanagan BF, Sample JT, Stewart JP Chemokine binding protein M3 of murine gammaherpesvirus 68 modulates the host response to infection in a natural host. PLoS Pathog., 2011-03-17;7(3):e1001321. 2011-03-17 [PMID: 21445235] (Mouse)
• Jung Y, Wang J, Schneider A, Sun YX, Koh-Paige AJ, Osman NI, McCauley LK, Taichman RS Regulation of SDF-1 (CXCL12) production by osteoblasts; a possible mechanism for stem cell homing. Bone, 2005-12-05;38(4):497-508. 2005-12-05 [PMID: 16337237] (Mouse)

FAQs for CXCL12/SDF-1 (DY460). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Cxcl12