Mouse Chemerin Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Chemerin Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse Chemerin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature Chemerin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Chemerin. Results obtained using natur...al mouse Chemerin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Chemerin.
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• Specificity: Natural and recombinant mouse Chemerin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Rarres2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Chemerin Quantikine ELISA Kit

• Chemerin
• RARRES2
• RAR-responsive protein TIG2
• retinoic acid receptor responder (tazarotene induced) 2
• retinoic acid receptor responder protein 2
• Tazarotene-induced gene 2 protein
• TIG-2
• TIG2HP10433

Background

Chemerin, also known as Tazarotene-induced Gene 2, (Tig-2), is a member of the Cystatin superfamily. Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin fold-containing domain, and a six aa C-terminal prosegment. It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades. Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Chemerin (MCHM00)(10)

Publications using MCHM00

• L Zhao, Y Yamaguchi, WJ Shen, J Morser, LLK Leung Dynamic and tissue-specific proteolytic processing of chemerin in obese mice PLoS ONE, 2018-08-30;13(8):e0202780. 2018-08-30 [PMID: 30161155] (Mouse)
• Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia Nat Commun, 2016-08-19;7(0):12528. 2016-08-19 [PMID: 27538380] (Mouse)
• Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S Endothelial cell-derived chemerin promotes dendritic cell transmigration. J Immunol, 2014-01-27;192(5):2366-73. 2014-01-27 [PMID: 24470498] (Mouse)
• The chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses. J. Exp. Med., 2012-07-02;209(8):1427-35. 2012-07-02 [PMID: 22753924] (Mouse)
• Monnier J, Lewen S, O'Hara E, Huang K, Tu H, Butcher EC, Zabel BA Expression, Regulation, and Function of Atypical Chemerin Receptor CCRL2 on Endothelial Cells. J. Immunol., 2012-06-13;189(2):956-67. 2012-06-13 [PMID: 22696441] (Mouse)
• Ernst MC, Haidl ID, Zúñiga LA, Dranse HJ, Rourke JL, Zabel BA, Butcher EC, Sinal CJ Disruption of the chemokine-like receptor-1 (CMKLR1) gene is associated with reduced adiposity and glucose intolerance. Endocrinology, 2011-12-20;153(2):672-82. 2011-12-20 [PMID: 22186410] (Mouse)
• Bondue B, Vosters O, de Nadai P, Glineur S, De Henau O, Luangsay S, Van Gool F, Communi D, De Vuyst P, Desmecht D, Parmentier M ChemR23 dampens lung inflammation and enhances anti-viral immunity in a mouse model of acute viral pneumonia. PLoS Pathog., 2011-11-03;7(11):e1002358. 2011-11-03 [PMID: 22072972] (Mouse)
• Demoor T, Bracke KR, Dupont LL The Role of ChemR23 in the Induction and Resolution of Cigarette Smoke-Induced Inflammation. J. Immunol., 2011-03-23;186(9):5457-67. 2011-03-23 [PMID: 21430224] (Mouse)
• Bauer S, Wanninger J, Schmidhofer S, Weigert J, Neumeier M, Dorn C, Hellerbrand C, Zimara N, Schaffler A, Aslanidis C, Buechler C Sterol regulatory element-binding protein 2 (SREBP2) activation after excess triglyceride storage induces chemerin in hypertrophic adipocytes. Endocrinology, 2010-11-17;152(1):26-35. 2010-11-17 [PMID: 21084441] (Mouse)
• Becker M, Rabe K, Lebherz C, Zugwurst J, Goke B, Parhofer KG, Lehrke M, Broedl UC Expression of human chemerin induces insulin resistance in the skeletal muscle but does not affect weight, lipid levels, and atherosclerosis in LDL receptor knockout mice on high-fat diet. Diabetes, 2010-08-19;59(11):2898-903. 2010-08-19 [PMID: 20724582] (Mouse)

FAQs for Chemerin (MCHM00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Rarres2