Mouse Adiponectin/Acrp30 DuoSet ELISA, 15 Plate

AdipoRon inhibits orthotopic pancreatic tumor growth and tumor proliferation in vivoMurine P-4313 and K-8484 cancer cells were orthotopically injected into syngeneic wildtype mice and allowed to grow for two weeks, after which mice were treated with vehicle or AdipoRon 5mg/kg/day for 2 weeks. (A) at endpoint, AdipoRon treated mice had a significantly reduced tumor size. (B) tumor proliferation measured by Ki67 positive tumor area per tumor section was significantly reduced after AdipoRon treatment. (C) no significant difference was detected in relative expression of AdipoR1 (white bars) and AdipoR2 (black bars) by quantitative real time PCR analysis between control and AdipoRon treated tumors. (D) ELISA analysis showed no significant difference in circulating serum adiponectin levels between the two groups of mice. (P-4313 filled symbols and K-8484 open symbols). Statistical analysis was performed using unpaired t-test (*P ≤ 0.05, **P ≤ 0.01). Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.19905), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Adiponectin/Acrp30 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Adipoq

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Adiponectin/Acrp30 DuoSet ELISA, 15 Plate

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Publications for Adiponectin/Acrp30 (DY1119)(57)

Publications using DY1119

• Goli, SH;Lim, JY;Basaran-Akgul, N;Templeton, SP; Adiponectin pathway activation dampens inflammation and enhances alveolar macrophage fungal killing via LC3-associated phagocytosis bioRxiv : the preprint server for biology 2024-06-26 [PMID: 38979340] (Mouse)
• Honce, R;Vazquez-Pagan, A;Livingston, B;Mandarano, AH;Wilander, BA;Cherry, S;Hargest, V;Sharp, B;Brigleb, PH;Kirkpatrick Roubidoux, E;Van de Velde, LA;Skinner, RC;McGargill, MA;Thomas, PG;Schultz-Cherry, S; Diet switch pre-vaccination improves immune response and metabolic status in formerly obese mice Nature microbiology 2024-04-18 [PMID: 38637722] (Mouse)
• Anderson, JM;Arnold, WD;Huang, W;Ray, A;Owendoff, G;Cao, L; Long-term effects of a fat-directed FGF21 gene therapy in aged female mice Gene therapy 2023-09-12 [PMID: 37699965] (Mouse)
• Blandin, A;Amosse, J;Froger, J;Hilairet, G;Durcin, M;Fizanne, L;Ghesquière, V;Prieur, X;Chaigneau, J;Vergori, L;Dray, C;Pradère, JP;Blandin, S;Dupont, J;Ducluzeau, PH;Dubois, S;Boursier, J;Cariou, B;Le Lay, S; Extracellular vesicles are carriers of adiponectin with insulin-sensitizing and anti-inflammatory properties Cell reports 2023-08-29 [PMID: 37605533] (Transgenic Mouse, Mouse)
• Deng, L;Wu, SA;Qi, L;Kersten, S; HILPDA is a lipotoxic marker in adipocytes that mediates the autocrine negative feedback regulation of triglyceride hydrolysis by fatty acids and alleviates cellular lipotoxic stress Molecular metabolism 2023-07-06 [PMID: 37422000] (Mouse)
• TS Huang, T Wu, YD Wu, XH Li, J Tan, CH Shen, SJ Xiong, ZQ Feng, SF Gao, H Li, WB Cai Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice Nature Communications, 2023-01-24;14(1):390. 2023-01-24 [PMID: 36693830] (Mouse)
• K Kobayashi, YT Tang, K Sasaki Paeoniflorin, a constituent of Kami-shoyo-san, suppresses blood glucose levels in postmenopausal diabetic mice by promoting the secretion of estradiol from adipocytes Oncogene, 2022-09-05;32(0):101335. 2022-09-05 [PMID: 36510583] (Mouse)
• L Orliaguet, T Ejlalmanes, A Humbert, R Ballaire, M Diedisheim, JB Julla, D Chokr, J Cuenco, J Michielett, J Charbit, D Lindén, J Boucher, C Potier, A Hamimi, S Lemoine, C Blugeon, P Legoix, S Lameiras, LG Baudrin, S Baulande, A Soprani, FA Castelli, F Fenaille, JP Riveline, E Dalmas, J Rieusset, JF Gautier, N Venteclef, F Alzaid Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling Nature Communications, 2022-08-30;13(1):5089. 2022-08-30 [PMID: 36042203] (Mouse)
• M Thomalla, A Schmid, J Hehner, S Koehler, E Neumann, U Müller-Lad, A Schäffler, T Karrasch Toll-like Receptor 7 (TLR7) Is Expressed in Adipocytes and the Pharmacological TLR7 Agonist Imiquimod and Adipocyte-Derived Cell-Free Nucleic Acids (cfDNA) Regulate Adipocyte Function International Journal of Molecular Sciences, 2022-07-30;23(15):. 2022-07-30 [PMID: 35955609] (Mouse)
• M Mohan Shre, S Wermelin, E Stener-Vic, I Wernstedt, A Benrick Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice International Journal of Molecular Sciences, 2022-04-29;23(9):. 2022-04-29 [PMID: 35563332] (Mouse)
• MR Spetz, C Isely, RM Gower Effect of fabrication parameters on morphology and drug loading of polymer particles for rosiglitazone delivery Journal of drug delivery science and technology, 2021-07-01;65(1):. 2021-07-01 [PMID: 35096148] (Mouse)
• JA Victorio, DM Guizoni, IN Freitas, TR Araujo, AP Davel Effects of High-Fat and High-Fat/High-Sucrose Diet-Induced Obesity on PVAT Modulation of Vascular Function in Male and Female Mice Frontiers in Pharmacology, 2021-09-10;12(0):720224. 2021-09-10 [PMID: 34566644] (Mouse)
• K Kuramoto, YJ Kim, JH Hong, C He The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding Cell Reports, 2021-05-25;35(8):109184. 2021-05-25 [PMID: 34038729] (Mouse)
• RDC da Cunha d, JJ Simão, VSD Silva, TM Farias, MM Cruz, VJ Antraco, L Armelin-Co, MI Alonso-Val Fish Oil Enriched in EPA, but Not in DHA, Reverses the Metabolic Syndrome and Adipocyte Dysfunction Induced by a High-Fat Diet Nutrients, 2021-02-26;13(3):. 2021-02-26 [PMID: 33652751] (Mouse)
• A Schmid, M Roderfeld, J Gehl, E Roeb, A Nist, HR Chung, T Stiewe, T Karrasch, A Schäffler C1q/TNF-Related Protein 3 (CTRP-3) Deficiency of Adipocytes Affects White Adipose Tissue Mass but Not Systemic CTRP-3 Concentrations International Journal of Molecular Sciences, 2021-02-07;22(4):. 2021-02-07 [PMID: 33562308] (Mouse)
• M Hoekstra, AB Ouweneel, J Price, R van der Ge, RJ van der Sl, JJ Geerling, JE Nahon, M Van Eck SR-BI deficiency disassociates obesity from hepatic steatosis and glucose intolerance development in high fat diet-fed mice The Journal of nutritional biochemistry, 2020-12-13;89(0):108564. 2020-12-13 [PMID: 33321184] (Mouse)
• XAMH van Dieren, T Sancerni, MC Alves-Guer, R Stienstra The role of uncoupling protein 2 in macrophages and its impact on obesity-induced adipose tissue inflammation and insulin resistance J Biol Chem, 2020-01-01;0(0):. 2020-01-01 [PMID: 33051201] (Mouse)
• EM Ahn, G Asamenew, HW Kim, SH Lee, SM Yoo, SM Cho, YS Cha, MS Kang Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds Nutrients, 2020-04-29;12(5):. 2020-04-29 [PMID: 32365527] (Mouse)
• RDC da Cunha d, MM Cruz, TM de Farias, VS da Silva, J de Jesus S, MM Telles, MIC Alonso-Val Fish oil reverses metabolic syndrome, adipocyte dysfunction, and altered adipokines secretion triggered by high-fat diet-induced obesity Physiol Rep, 2020-02-01;8(4):e14380. 2020-02-01 [PMID: 32109344] (Mouse)
• XAMH van Dieren, MA de la Rosa, A Georgiadi, F Mattijssen, W Dijk, M van Weeghe, R Singh, JW Borst, R Stienstra, S Kersten HILPDA Uncouples Lipid Droplet Accumulation in Adipose Tissue Macrophages from Inflammation and Metabolic Dysregulation Cell Rep, 2020-02-11;30(6):1811-1822.e6. 2020-02-11 [PMID: 32049012] (Mouse)
• CH Val, MC de Oliveir, DR Lacerda, A Barroso, NV Batista, Z Menezes-Ga, DRR de Assis, AT Cramer, F Brant, MM Teixeira, D Glória Sou, AM Ferreira, FS Machado SOCS2 modulates adipose tissue inflammation and expansion in mice J. Nutr. Biochem., 2019-11-23;76(0):108304. 2019-11-23 [PMID: 31816561] (Mouse)
• NJ Queen, AA Boardman, RS Patel, JJ Siu, X Mo, L Cao Environmental enrichment improves metabolic and behavioral health in the BTBR mouse model of autism Psychoneuroendocrinology, 2019-10-15;111(0):104476. 2019-10-15 [PMID: 31648110] (Mouse)
• TDSM Farias, RID Paixao, MM Cruz, RDCDC de Sa, JJ Simão, VJ Antraco, MIC Alonso-Val Melatonin Supplementation Attenuates the Pro-Inflammatory Adipokines Expression in Visceral Fat from Obese Mice Induced by A High-Fat Diet Cells, 2019-09-06;8(9):. 2019-09-06 [PMID: 31489938] (Mouse)
• S Musovic, CS Olofsson Adrenergic stimulation of adiponectin secretion in visceral mouse adipocytes is blunted in high-fat diet induced obesity Sci Rep, 2019-07-23;9(1):10680. 2019-07-23 [PMID: 31337827] (Mouse)
• TA Diniz, JCJ Aquino Jún, FC Mosele, C Cabral-San, EA Lima Junio, AAS Teixeira, FS Lira, JC Rosa Neto Exercise-induced AMPK activation and IL-6 muscle production are disturbed in adiponectin knockout mice Cytokine, 2019-03-20;119(0):71-80. 2019-03-20 [PMID: 30903866] (Mouse)
• V Stroikova, A Fischer, M Bockstahle, AM Müller, HA Katus, Z Kaya Adiponectin deficiency has no effect in murine autoimmune myocarditis Cytokine, 2019-02-01;116(0):139-149. 2019-02-01 [PMID: 30716658] (Mouse)
• K Song, C Gras, G Capin, N Gimber, M Lehmann, S Mohd, D Puchkov, M Rödiger, I Wilhelmi, O Daumke, J Schmoranze, A Schürmann, M Krauss A SEPT1-based scaffold is required for Golgi integrity and function J. Cell. Sci., 2019-02-01;132(3):. 2019-02-01 [PMID: 30709970] (Mouse)
• K Drareni, R Ballaire, S Barilla, MJ Mathew, A Toubal, R Fan, N Liang, C Chollet, Z Huang, M Kondili, F Foufelle, A Soprani, R Roussel, JF Gautier, F Alzaid, E Treuter, N Venteclef GPS2 Deficiency Triggers Maladaptive White Adipose Tissue Expansion in Obesity via HIF1A Activation Cell Rep, 2018-09-11;24(11):2957-2971.e6. 2018-09-11 [PMID: 30208320] (Mouse)
• LA Biondo, HA Batatinha, CO Souza, AAS Teixeira, LS Silveira, MI Alonso-Val, LM Oyama, MJ Alves, M Seelaender, JCR Neto Metformin Mitigates Fibrosis and Glucose Intolerance Induced by Doxorubicin in Subcutaneous Adipose Tissue Front Pharmacol, 2018-05-08;9(0):452. 2018-05-08 [PMID: 29867463] (Mouse)
• SL Christian, NK Pallegar, RJ Brown, AM Viloria-Pe Collagen overlays can inhibit leptin and adiponectin secretion but not lipid accumulation in adipocytes PeerJ, 2018-04-27;6(0):e4641. 2018-04-27 [PMID: 29719734] (Mouse)
• RC Gaspar, VR Muñoz, GP Formigari, GK Kuga, SCBR Nakandakar, JD Botezelli, ASR da Silva, DE Cintra, LP de Moura, ER Ropelle, JR Pauli Acute physical exercise increases the adaptor protein APPL1 in the hypothalamus of obese mice Cytokine, 2018-04-26;110(0):87-93. 2018-04-26 [PMID: 29705396] (Mouse)
• M Stadion, K Schwerbel, A Graja, C Baumeier, M Rödiger, W Jonas, C Wolfrum, H Staiger, A Fritsche, HU Häring, N Klöting, M Blüher, P Fischer-Po, TJ Schulz, HG Joost, H Vogel, A Schürmann Increased Ifi202b/IFI16 expression stimulates adipogenesis in mice and humans Diabetologia, 2018-02-24;0(0):. 2018-02-24 [PMID: 29478099] (Mouse)
• M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta , A Schürmann Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes Mol Metab, 2017-11-22;0(0):. 2017-11-22 [PMID: 29203237] (Mouse)
• F Jahandideh, S Chakrabart, ST Davidge, J Wu Egg white hydrolysate shows insulin mimetic and sensitizing effects in 3T3-F442A pre-adipocytes PLoS ONE, 2017-10-03;12(10):e0185653. 2017-10-03 [PMID: 28972997] (Mouse)
• C Brännmark, W Lövfors, AM Komai, T Axelsson, MF El Hachman, S Musovic, A Paul, E Nyman, CS Olofsson Mathematical modeling of white adipocyte exocytosis predicts adiponectin secretion and quantifies the rate of vesicle exo- and endocytosis J. Biol. Chem., 2017-09-25;0(0):. 2017-09-25 [PMID: 28972187] (Mouse)
• MJ Haley, G Mullard, KA Hollywood, GJ Cooper, WB Dunn, CB Lawrence Adipose tissue, metabolic and inflammatory responses to stroke are altered in obese mice Dis Model Mech, 2017-08-10;0(0):. 2017-08-10 [PMID: 28798136] (Mouse)
• A Imai, K Satoi, E Fujimoto, K Sato Inducing maternal inflammation promotes leptin production in offspring but does not improve allergic symptoms in a mouse model of allergic rhinitis Heliyon, 2017-06-22;3(6):e00327. 2017-06-22 [PMID: 28707000] (Mouse)
• A Aoki-Yoshi, S Saito, T Tsuruta, A Ohsumi, H Tsunoda, K Sonoyama Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in�vitro Biochem. Biophys. Res. Commun., 2017-05-27;0(0):. 2017-05-27 [PMID: 28559134] (Mouse)
• LL Ventura, NC Fortes, HC Santiago, MV Caliari, MA Gomes, DR Oliveira Obesity-induced diet leads to weight gain, systemic metabolic alterations, adipose tissue inflammation, hepatic steatosis, and oxidative stress in gerbils (Meriones unguiculatus) PeerJ, 2017-03-02;5(0):e2967. 2017-03-02 [PMID: 28265495] (Gerbil)
• Camila O Souza Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPAR?-Dependent AMPK Activation J. Cell. Physiol, 2017-03-24;0(0):. 2017-03-24 [PMID: 27925195] (Mouse)
• Travis B McMurphy Hepatic Expression of Adenovirus 36 E4ORF1 Improves Glycemic Control and Promotes Glucose Metabolism via AKT Activation Diabetes, 2016-11-30;0(0):. 2016-11-30 [PMID: 27903748] (Mouse)
• H Lee, H Li, M Noh, JH Ryu Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes Int J Mol Sci, 2016-04-08;17(4):. 2016-04-08 [PMID: 27070585] (Mouse)
• Fairbridge N, Southall T, Ayre D, Komatsu Y, Raquet P, Brown R, Randell E, Kovacs C, Christian S Loss of CD24 in Mice Leads to Metabolic Dysfunctions and a Reduction in White Adipocyte Tissue. PLoS ONE, 2015-11-04;10(11):e0141966. 2015-11-04 [PMID: 26536476] (Mouse)
• Chakrabarti S, Wu J Milk-derived tripeptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) promote adipocyte differentiation and inhibit inflammation in 3T3-F442A cells. PLoS ONE, 2015-02-25;10(2):e0117492. 2015-02-25 [PMID: 25714093] (Mouse)
• Kim J, Sohn J, Lee J, Park J Obesity increases airway hyperresponsiveness via the TNF-alpha pathway and treating obesity induces recovery. PLoS ONE, 2015-02-06;10(2):e0116540. 2015-02-06 [PMID: 25658739] (Mouse)
• Barra , Nicole G, Palanivel , Rengasam, Denou , Emmanuel, Chew , Marianne, Gillgrass , Amy, Walker , Tina D, Kong , Josh, Richards , Carl D, Jordana , Manel, Collins , Stephen, Trigatti , Bernardo, Holloway , Alison C, Raha , Sandeep, Steinberg , Gregory, Ashkar , Ali A Interleukin-15 modulates adipose tissue by altering mitochondrial mass and activity. PLoS ONE, 2014-12-17;9(12):e114799. 2014-12-17 [PMID: 25517731] (Mouse)
• Moreno M, De Laquila R, Okuda M, Lira F, de Souza G, de Souza C, Telles M, Ribeiro E, do Nascimento C, Oyama L Metabolic profile response to administration of epigallocatechin-3-gallate in high-fat-fed mice. Diabetol Metab Syndr, 2014-08-12;6(1):84. 2014-08-12 [PMID: 25147582] (Mouse)
• Lee M, Lim C, Lee Y, Park J, Sonn S, Lee M, Jung I, Jeong S, Jeon S, Lee M, Oh K, Yang Y, Kim J, Choi H, Jeong W, Jeong T, Yoon W, Kim H, Choi J, Oh G The adipokine Retnla modulates cholesterol homeostasis in hyperlipidemic mice. Nat Commun, 2014-07-15;5(0):4410. 2014-07-15 [PMID: 25022542] (Mouse)
• Li Y, Hsieh C Lactoferrin dampens high-fructose corn syrup-induced hepatic manifestations of the metabolic syndrome in a murine model. PLoS ONE, 2014-05-09;9(5):e97341. 2014-05-09 [PMID: 24816278] (Mouse)
• Liu , Xianglan, McMurphy , Travis, Xiao , Run, Slater , Andrew, Huang , Wei, Cao , Lei Hypothalamic gene transfer of BDNF inhibits breast cancer progression and metastasis in middle age obese mice. Mol Ther, 2014-03-18;22(7):1275-84. 2014-03-18 [PMID: 24637454] (Mouse)
• Dorn C, Engelmann J, Saugspier M, Koch A, Hartmann A, Muller M, Spang R, Bosserhoff A, Hellerbrand C Increased expression of c-Jun in nonalcoholic fatty liver disease. Lab Invest, 2014-02-03;94(4):394-408. 2014-02-03 [PMID: 24492282] (Mouse)
• Menezes-Garcia Z, Oliveira M, Lima R, Soriani F, Cisalpino D, Botion L, Teixeira M, Souza D, Ferreira A Lack of platelet-activating factor receptor protects mice against diet-induced adipose inflammation and insulin-resistance despite fat pad expansion. Obesity (Silver Spring), 2013-12-14;22(3):663-72. 2013-12-14 [PMID: 24339378] (Mouse)
• Gauvreau D, Gupta A, Fisette A, Tom F, Cianflone K Deficiency of C5L2 increases macrophage infiltration and alters adipose tissue function in mice. PLoS ONE, 2013-04-22;8(4):e60795. 2013-04-22 [PMID: 23630572] (Mouse)
• Elevated levels of circulating IL-18BP and perturbed regulation of IL-18 in schizophrenia. J Neuroinflammation, 2012-08-22;9(0):206. 2012-08-22 [PMID: 22913567] (Human)
• Freeman , Linnea R, Zhang , Le, Dasuri , Kalavath, Fernandez-Kim , Sun-Ok, Bruce-Keller , Annadora, Keller , Jeffrey Mutant amyloid precursor protein differentially alters adipose biology under obesogenic and non-obesogenic conditions. PLoS ONE, 2012-08-17;7(8):e43193. 2012-08-17 [PMID: 22912823] (Mouse)
• Pini M, Rhodes DH, Castellanos KJ Rosiglitazone improves survival and hastens recovery from pancreatic inflammation in obese mice. PLoS ONE, 2012-07-16;7(7):e40944. 2012-07-16 [PMID: 22815875] (Mouse)
• Miegueu P, Cianflone K, Richard D, St-Pierre D Effect of secretin on preadipocyte, differentiating and mature adipocyte functions. Int J Obes (Lond), 2012-05-08;37(3):366-74. 2012-05-08 [PMID: 22565418] (Mouse)

FAQs for Adiponectin/Acrp30 (DY1119). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Adipoq