Monoamine Oxidase B Antibody (5M8A5)

Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Mouse testis using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Rat lung using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Human colon using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Simple Western: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Monoamine Oxidase B Antibodies (NBP3-15411), ProteinSimple Western Blot on Jess Instrument. 1 microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human liver tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human tonsil tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human colon carcinoma tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Immunohistochemistry analysis of Monoamine Oxidase B in paraffin-embedded human kidney tissue using Monoamine Oxidase B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ELISA, IHC
• Clone: 5M8A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Monoamine Oxidase B Antibody (5M8A5) Summary

• Description: Novus Biologicals Rabbit Monoamine Oxidase B Antibody (5M8A5) (NBP3-15411) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338). MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFR
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAOB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Simple Western Validated from a verified customer review.
  • Western Blot 1:500 - 1:1000
• Application Notes: See Simple Western Antibody Database for Simple Western validation
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Monoamine Oxidase B Antibody (5M8A5)

• adrenalin oxidase
• amine oxidase [flavin-containing] B
• EC 1.4.3
• EC 1.4.3.21
• EC 1.4.3.4
• MAO, brain
• MAO, platelet
• MAOB
• MAO-B
• MGC26382
• Monoamine Oxidase B
• Monoamine oxidase type B
• tyramine oxidase

Background

Monoamine Oxidase B is encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Monoamine Oxidase B Antibody (NBP3-15411) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Stella Dracheva Simple Western Human 04/06/2023

Summary

• Application: Simple Western
• Sample Tested: Human Brain Tissue
• Species: Human
• Lot: 4000000641

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: MAOB