MOG Antibody [Unconjugated]

MOG was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human MOG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2395) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MOG Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MOG
  Gly30-Gly154 (Asp131Ala)
  Accession # Q8IYG5
• Specificity: Detects human MOG in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MOG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MOG Antibody [Unconjugated]

• MGC26137
• MOG Ig-AluB
• MOG
• MOGIG2
• myelin oligodendrocyte glycoprotein
• myelin-oligodendrocyte glycoprotein

Background

MOG is an Ig superfamily member found exclusively in myelinating oligodendrocytes in the central nervous system. Multiple isoforms of human MOG exist. The amino-terminal extracellular domain of human MOG shares 92% and 91% amino acid sequence homology with the corresponding regions of the mouse and rat protein, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MOG Antibody (AF2395)(1)

Publication using AF2395

• Wong , Agnes W, Xiao , Junhua, Kemper , Dennis, Kilpatrick , Trevor J, Murray , Simon S Oligodendroglial expression of TrkB independently regulates myelination and progenitor cell proliferation. J Neurosci, 2013-03-13;33(11):4947-57. 2013-03-13 [PMID: 23486965] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MOG
• Uniprot:
  • Q8IYG5()