MNX1/HLXB9 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

MNX1/HLXB9 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human MNX1/HLXB9.

  Source: E. coli

  Amino Acid Sequence: NFRIDALLAVDPPRAASAQSAPLALVTSLAAAASGTGG

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: MNX1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-57703.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for MNX1/HLXB9 Recombinant Protein Antigen

• HB9
• HLXB9motor neuron and pancreas homeobox protein 1
• homeobox HB9
• HOXHB9Homeobox protein HB9
• motor neuron and pancreas homeobox 1
• SCRA1homeo box HB9

Background

The homeobox gene Hb9/Hlxb9, is expressed selectively by motor neurons (MNs) in the developing vertebrate CNS. Hb9, which encodes a factor central to MN development regulates neuronal fate by restricting expression of Lim3 and Even-skipped (Eve), two homeodomain (HD) proteins required for development of distinct neuronal classes. In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons. Hb9 is also a transcription factor expressed in lymphoid and pancreatic tissue. In humans, HB9 mutation causes an autosomal dominant form of sacral agenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MNX1