MMR/CD206/Mannose Receptor Antibody [Unconjugated]

Images

 
Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

MMR/CD206/Mannose Receptor Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMR/CD206
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897
Specificity
Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MMR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MRC1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2534 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody [Unconjugated]

  • CD206
  • CLEC13D
  • CLEC13Dmacrophage mannose receptor 1
  • C-type lectin domain family 13 member D
  • mannose receptor, C type 1
  • MMR
  • MMRCD206 antigen
  • MRC1

Background

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF2534
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Publications for MMR/CD206/Mannose Receptor Antibody (AF2534)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Bioassay, ELISA Capture, Flow Cytometry, IHC, IHC-Fr, IHC-P.


Filter By Application
Bioassay
(1)
ELISA Capture
(1)
Flow Cytometry
(1)
IHC
(9)
IHC-Fr
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(10)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF2534 Applications Species
BS Finlin, H Memetimin, AL Confides, B Zhu, PM Westgate, EE Dupont-Ver, PA Kern Macrophages expressing uncoupling protein 1 increase in adipose tissue in response to cold in humans Scientific Reports, 2021;11(1):23598. 2021 [PMID: 34880313] (IHC, Human) IHC Human
Z Gao, A Lu, AC Daquinag, Y Yu, M Huard, C Tseng, X Gao, J Huard, MG Kolonin Partial Ablation of Non-Myogenic Progenitor Cells as a Therapeutic Approach to Duchenne Muscular Dystrophy Biomolecules, 2021;11(10):. 2021 [PMID: 34680151] (IHC, Mouse) IHC Mouse
S Taylor, M Whitfield, J Barratt, A Didangelos The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix J. Immunol., 2020;0(0):. 2020 [PMID: 32917786] (IHC, Human) IHC Human
K Kosmac, M Gonzalez-F, MM McDermott, SH White, RG Walton, RL Sufit, L Tian, L Li, MR Kibbe, MH Criqui, JM Guralnik, T S Polonsky, C Leeuwenbur, L Ferrucci, CA Peterson Correlations of Calf Muscle Macrophage Content With Muscle Properties and Walking Performance in Peripheral Artery Disease J Am Heart Assoc, 2020;9(10):e015929. 2020 [PMID: 32390569] (IHC, Human) IHC Human
CY Cui, RK Driscoll, Y Piao, CW Chia, M Gorospe, L Ferrucci Skewed macrophage polarization in aging skeletal muscle Aging Cell, 2019;0(0):e13032. 2019 [PMID: 31478346] (IHC-Fr, Human) IHC-Fr Human
B Liu, AT Hutchison, CH Thompson, K Lange, LK Heilbronn Markers of adipose tissue inflammation are transiently elevated during intermittent fasting in women who are overweight or obese Obes Res Clin Pract, 2019;0(0):. 2019 [PMID: 31302012] (IHC-P, Human) IHC-P Human
MC Nielsen, MN Andersen, N Rittig, S Rødgaard-H, H Grønbaek, SK Moestrup, HJ Møller, A Etzerodt The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 31242338] (ELISA Capture, Human) ELISA Capture Human
T Zhang, L Liu, W Lai, Y Zeng, H Xu, Q Lan, P Su, Z Chu Interaction with tumor?associated macrophages promotes PRL?3?induced invasion of colorectal cancer cells via MAPK pathway?induced EMT and NF??B signaling?induced angiogenesis Oncol. Rep., 2019;0(0):. 2019 [PMID: 30864736] (Flow Cytometry, Human) Flow Cytometry Human
R Yang, M Wu, S Lin, RP Nargund, X Li, T Kelly, L Yan, G Dai, Y Qian, Q Dallas-Yan, PA Fischer, Y Cui, X Shen, P Huo, DD Feng, MD Erion, DE Kelley, J Mu A glucose-responsive insulin therapy protects animals against hypoglycemia JCI Insight, 2018;3(1):. 2018 [PMID: 29321379] (Bioassay) Bioassay
YF Malada-Ede, N Hadad, R Levy Regulatory role of cytosolic phospholipase A2 alpha in the induction of CD40 in microglia J Neuroinflammation, 2017;14(1):33. 2017 [PMID: 28187742] (IHC, Mouse) IHC Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol MRC1
Uniprot