PMS1 Antibody (PCRP-PMS1-2E11)

Immunocytochemistry/Immunofluorescence: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PMS1 Antibody (PCRP-PMS1-2E11) [CF488]. PMS1 localized to nucleoplasm and nuclear bodies. Microtubules stained with CF640R.

Flow Cytometry: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Flow cytometric analysis of PFA-fixed HeLa cells. PMS1 antibody (PCRP-PMS1-2E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMS1 Antibody (PCRP-PMS1-2E11).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, PA
• Clone: PCRP-PMS1-2E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PMS1 Antibody (PCRP-PMS1-2E11) Summary

• Immunogen: Recombinant full-length human PMS1 protein (Uniprot: P54277)
• Localization: Nucleus, Nuclear bodies
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMS1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: concentration is assay dependent
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 105.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14142)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for PMS1 Antibody (PCRP-PMS1-2E11)

• DKFZp781M0253
• FLJ98259
• HNPCC3
• hPMS1
• human homolog of yeast mutL
• mismatch repair gene PMSL1
• PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
• PMS1 protein homolog 1
• PMSL1DNA mismatch repair protein PMS1
• postmeiotic segregation increased (S. cerevisiae) 1
• rhabdomyosarcoma antigen MU-RMS-40.10B
• rhabdomyosarcoma antigen MU-RMS-40.10E

Background

PMS1 was identified by its homology to a yeast protein involved in DNA mismatch repair. A role for this protein in mismatch repair has not been proven. However, the protein forms heterodimers with MLH1, a DNA mismatch repair protein, and some cases of hereditary nonpolyposis colorectal cancer have been found to have mutations in the gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMS1
• Uniprot:
  • P54277(Human)