![Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/PMS2-Antibody-163C1251-Western-Blot-NB100-56554-img0003.jpg "Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clone | 163C1251 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Mouse PMS2 Antibody (163C1251) - BSA Free (NB100-56554) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-PMS2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 623-639 SSLAKRIKQLHHEAQQS of human PMS2; GenBank No. NP_000526.1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PMS2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | An approx. 85 kDa band should be observed. This antibody is Cytof ready. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
| Publication using NB100-56554 | Applications | Species |
|---|---|---|
| Inanaga S, Igase M, Sakai Y et al. Mismatch repair deficiency in canine neoplasms Veterinary pathology 2021-09-19 [PMID: 34538134] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PMS2 Antibody (NB100-56554)Find related products by research area.
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![Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using PMS2 antibody at 5 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/PMS2-Antibody-163C1251-Immunohistochemistry-Paraffin-NB100-56554-img0005.jpg "Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using PMS2 antibody at 5 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")