Anti-MMR/CD206/Mannose Receptor Antibodies: Novus Biologicals

AF2535

MMR/CD206/Mannose Receptor Antibody [Unconjugated]

(2 Reviews)

100 μg

AF2535

HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # <a class=

NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), and confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); and 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), and MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology and confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), and VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) and images are merged in (k, p). Scale = 10 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

M1 and M2 phenotype in spinal cord after intraplantar IL-1 beta . Wild-type (WT) and LysM-G protein–coupled receptor kinase (GRK)2+/− mice received an intraplantar injection of 1 ng IL-1 beta . At 15 hours after injection, spinal cord was collected, and frozen sections of (A) lumbar spinal cord (L2 to L5) and as control (B) thoracic spinal cord (T6 to T10) were stained for M1 (CD16/32) and M2 (CD206 and arginase-I) phenotypic markers. A representative example of M1 and M2 staining in the dorsal horn of one of the four mice per group is displayed. Scale bar indicates 20 μm. (C) Quantification of microglia/macrophages expressing M1 and M2 phenotypic markers in spinal cord from WT and LysM-GRK2+/− mice. Expression was quantified in approximately 10 to 15 dorsal horns of spinal cords per group (4 mice per group). The level of expression in the lumbar or thoracic area from control WT mice was set at 100%. Data are expressed as means ± SEM. **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22731384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma and LPS, (C) cells stimulated with IL-4. (D–F) Bone-marrow-derived cells isolated from Mus and stained for F4/80 (green). (D) unstimulated cells, (E) cells stimulated with IFN gamma and LPS, and (F) cells stimulated with IL-4. Scale bar = 50 μm. (G) Acomys ear tissue at D15 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). (H) Mus ear tissue at D7 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). Scale bar = 50 μm.DOI://dx.doi.org/10.7554/eLife.24623.012 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28508748), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta . Cells co-expressing VEGFR3 and LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) and LYVE1 (d′, white) internalize A beta 1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) and MRC1 (e′, white) internalize A beta 1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) and LYVE1 (f′, white) internalize A beta 1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta 1-40. Scale = 60 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro activation assays shows Acomys macrophages can be polarized to express different markers.(A–I) Bone-marrow-derived macrophages isolated from Acomys femurs are cultured with no cytokines (unstimulated, A, D, G) with IFN gamma +LPS (M1, B, E, H) or with IL-4 (M2, C, F, I). Immunocytochemistry for the pan-macrophage marker CD11b (green) (A–C), for the M1 macrophage marker CD86 (green) and the M2 macrophage marker Arginase 1 (red) (D–F), or CD206 (red) (G–I). (J–R). Bone-marrow-derived macrophages were isolated from Mus femurs and cultured with no cytokines (J, M, P) with IFN gamma and LPS (K, N, Q) or with IL4 (L, O, R) as above. Immunocytochemistry was performed for CD11b (green) (J–K), for CD86 (green) and Arginase 1 (red) (M–O), and CD206 (red) (P–R). Nuclei were counterstained with DAPI (grey) in all panels. Scale bars = 50 μm. Images are representative of n = 3 technical replicates.DOI://dx.doi.org/10.7554/eLife.24623.011Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma and LPS, (C) cells stimulated with IL-4. (D–F) Bone-marrow-derived cells isolated from Mus and stained for F4/80 (green). (D) unstimulated cells, (E) cells stimulated with IFN gamma and LPS, and (F) cells stimulated with IL-4. Scale bar = 50 μm. (G) Acomys ear tissue at D15 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). (H) Mus ear tissue at D7 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). Scale bar = 50 μm.DOI://dx.doi.org/10.7554/eLife.24623.012 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28508748), licensed under a CC-BY license. Not internally tested by R&D Systems.

Microglial activation was attenuated by Hv1 deletion following LPC-induced demyelination. a Representative images of Iba-1 immunostaining in the CC of WT and Hv1−/− mice (scale bar, 200 μm). b Quantification of the number of microglia per high-power field (HPF) in the CC. Each point of WT and Hv1−/− mice, N = 5-7 mice. c Representative images of Iba-1 morphology and the corresponding 3D reconstructions (scale bars, magnified images, 20 μm; 3D reconstruction images, 5um) d Quantification analysis of the soma of microglia. Each point of WT and Hv1−/− mice, N = 4-6 mice, 6-15 cells per mouse. e Representative images of Iba-1 and CD16/32 co-localization in the CC of WT and Hv1−/− mice (scale bar, 50 μm; magnified images, 20 μm). f Quantification of the ratio of CD16/32+/Iba-1+. Each point of WT mice, N = 5-8 mice; Hv1−/− mice, N = 5-8 mice. g Representative images of Iba-1 and CD206 co-localization in the CC of WT and Hv1−/− mice (scale bar, 50 μm; magnified images, 20 μm). h Quantification of the ratio of CD206+/Iba-1+. Each point of WT mice, N = 6-7 mice; Hv1−/− mice, N = 5-6 mice. Data are shown as mean ± SD, *P < 0.05, ***P<0.001, two-way ANOVA with Dunnett’s post hoc test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33158440), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha -GFP, green) near kdr-l positive ( alpha -RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries and veins. NG2 (red) labels pericytes and smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, and Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), and VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single and double-labelled cells in 2-month old mouse meninges. VEGFR3 and LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 and LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Representative images of HE, M3/84 (macrophages) and MR (CD206) immunohistochemistry. In control mice, no plaques with macrophages were observed, while fibrous/fibroatheromatous plaques were present in the aortas extracted from ApoE-KO mice. The lesions (fatty streaks and fibrous plaques) showed high amounts of MR+ macrophages (100 μm (bars), vascular lumen (L), intima (arrow), media (asterisk), adventitia (arrowhead)) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28470406), licensed under a CC-BY license. Not internally tested by R&D Systems.

TAMs along the beam paths show a mixed M2-like/M1-like phenotype and expression of phagocytosis markers. (A) Double staining for CD68 (red) and CD206 (green) plus DAPI (blue) shows at 7 days post-MRT that many of the underlined TAMs are positive for both markers, indicating their inclination towards an M2-like phenotype. Dashed white lines demarcate the border between the tumour (lower part) and the normal tissue (upper part). (B) Triple staining for CD68 (red), CD206 (green) and Dectin-1 (grey) shows an abundant triple positive TAM population along the beam paths, indicating their inclination towards a phagocytic phenotype. (C) Double staining for CD68 (red) and Ly6C (cyan) reveals a partial M1-like TAM population and, at the same time, reveals cells that are not double positive, indicating the presence of recruited monocytes. Dashed yellow lines indicate a macrophage cluster along a microbeam path. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35453485), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hypothermia promotes a shift of microglia/macrophages towards an anti‐inflammatory phenotype in aged female mice 7 days after ischemic stroke. Mice were subjected to distal MCAO (dMCAO) or sham surgery. Normothermia (NT) or hypothermia (HT) was induced for 50 min immediately after dMCAO. Coronal brain sections were stained for Iba‐1 (a microglial/macrophage marker) and CD206 (an anti‐inflammatory marker) or CD16 (a pro‐inflammatory marker). (A) Representative images of Iba1/CD16 and Iba1/CD206 immunofluorescence staining in the ipsilateral peri‐infarct cortex (CTX) regions. (B) Representative images showing Iba1/CD16 and Iba1/CD206 immunofluorescence staining in the ipsilateral peri‐infarct striatum (STR) regions. (C) Representative magnified 3‐dimensional images of Iba1/CD16 and Iba1/CD206 staining. (D) Quantification of the total number of Iba1+ cells (upper panel), Iba1+/CD16+ cells (middle panel), and Iba1+/CD206+ cells (lower panel). The number of double‐positive cells was expressed as the number over 100 Iba1+ cells. n = 5 for sham (S), n = 6 for NT and n = 6 for HT. (E) Pearson correlation analysis of NeuN+ cells with Iba1+/CD206+ cells in the CTX region, n = 6 per group. (F) Pearson correlation analysis of beta ‐APP+ cells with Iba1+/CD206+ cells in the CTX region, n = 6 per group. Scale bar: 50 μm. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 NT vs. HT, ns, no significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36341958), licensed under a CC-BY license. Not internally tested by R&D Systems.

HCK knockout reduced kidney fibrosis in uIRIx model with regulation of macrophage activation.A Schematic diagram of strategy of uIRIx models. B Urine ACR and serum BUN for WT and HCK KO uIRIx mice. C Representative images of H&E, COL1A1 IF and Masson trichrome staining from WT and KO uIRIx kidneys. Morphometric quantification (n = 5 animals; 5 random fields/animal) of the COL1A1 (top) and Masson (lower) positive area. D Representative IF staining images and quantification of from kidneys of HCK and F4/80 macrophage from WT and HCK KO uIRIx kidneys. E Western blot and quantification of M1 and M2 macrophage markers from WT and HCK KO mice kidneys at 28 days post uIRIx. F Representative IF staining images and quantification of LC3 in macrophages from WT & HCK KO uIRIx kidneys. B, C and E*p < 0.05 with t test. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37463911), licensed under a CC-BY license. Not internally tested by R&D Systems.

Quantification of the number of perivascular macrophages (PVMs). The number of PVMs in the cerebral cortex was counted and compared between the control and CB-NP exposure groups. (A–F) Low-magnification images to quantify the number of PVMs using MMR expression. (A,D) are merged pictures of (B,C,E,F), respectively. Arrowheads indicate MMR-positive PVMs. (G) Number of PVMs in the cerebral cortex. Values are expressed as the mean ± SD. Abbreviation: Carbon black nanoparticle (CB-NP), Cerebral cortex (Cx), Corpus callosum (CC), and Striatum (Str), Standard deviation (SD). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35391824), licensed under a CC-BY license. Not internally tested by R&D Systems.

RP-832c peptide levels significantly decreased in CD206 + macrophages and the profibrotic markers alpha -SMA and TGF-beta 1 without affecting Siglec-F-positive alveolar macrophages. (A) Representative 20× and 40× images of immunohistochemistry staining using anti-Siglec-F and anti-CD206 antibodies in lung tissues of naïve mice, as well as BLM-challenged vehicle/RP-832c-treated mice. The calibration bar represents 10 µm. (B) Representative 20× and 40× images of immunofluorescence staining of lung tissues using antibodies for alpha -SMA (red) and TGF beta -1 (green). The calibration bar represents 20 µm. (C–F) Bar graphs showing the quantifications of the IHC (Siglec-F & CD206) and IF ( alpha -SMA & TGF-beta 1) staining of lung tissues. Quantifications was done using Metamorph imaging software. S. E. **** p < 0.0001, *** p < 0.001, ** p < 0.01 and * p < 0.05 were considered significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37174654), licensed under a CC-BY license. Not internally tested by R&D Systems.

RP-832c peptide is comparable to Pirfenidone in decreasing fibrosis in an established late model of BLM-induced lung fibrosis. (A) A schematic of the animal study in which mice were challenged with a 2.5 U/kg body weight dose of BLM at day 0. A total of 14 days post-BLM challenge, mice were treated with either a vehicle, 10 mg/kg RP-832c (QD), 30 mg/kg Pirfenidone (Q3D), or a combination of RP-832c and Pirfenidone for an additional 21 days. (B) Histological analysis of lung tissues. The upper panel shows representative 2× images of Masson’s Trichrome-stained whole lung tissue, the middle panel contains representative 20× images, and the lower panel shows 10× images representing anti-CD206 immunohistochemical staining of lung tissue. (C) Modified Ashcroft scoring of each treatment group, determined after 21 days of treatment. n = 6 per treatment group. S. E. ** p < 0.001, and * p < 0.05 were considered significant. (D) Quantification of the anti-CD206 IHC-stained lung tissues. The bar represents 10 µm. n = 6 per treatment group. S. E. **** p < 0.0001, ** p < 0.001, and * p < 0.05 were considered significant. (E) Body weights of the mice measured over the course of treatment in each group. n = 6 per treatment group. (F) Lung weights of the mice measured at the end of the study. n = 6 per treatment group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37174654), licensed under a CC-BY license. Not internally tested by R&D Systems.

DSS-treated TLR4-SNP mice exhibit reduced M2a markers in the colon and reduced beta -hydroxybutyrate ( beta -HB) levels in the sera. (A) M2a gene expression measured in the MC of male WT vs TLR4-SNP mice, comparing control, Day 9, and Day 11 samples by qRT-PCR. One-way ANOVA with Sidak multiple comparisons test. *P < 0.05; **P < 0.01; ****P < 0.0001. Data are derived from two independent experiments. In total, control WT n = 11; control TLR4-SNP n = 15; WT Day 9 n = 13, Day 11 n = 13; TLR4-SNP Day 9 n = 15, Day 11 n = 15. (B) M2a (Ym1, Arg1) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data are representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. (C) M2a (Mrc1, PPAR gamma ) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. (D) beta -HB concentration measured in the sera of control and DSS-treated, WT vs TLR4-SNP male mice at Days 9 and 11. Two-way ANOVA with Sidak multiple comparisons. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Data are derived from three independent experiments in which, together, control WT n = 12; control TLR4-SNP n = 13; WT Day 11 n = 10; TLR4-SNP Day 11 n = 10. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37768050), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vivo experiment of TA gel in the mouse a the protocol of in vivo experiment; b in vivo fluorescence images of the TA gel at different implantation time points; c representative WB stripes of iNOS and CD206 expressions in the stroke mice; quantitative WB results of d iNOS and e CD206; ethological results of f balance beam and g rotarod tests Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37908012), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro cell culture a schematic diagram of BV2 cell culture; b BV2 cell viabilities treated with different TAG dilutions before and after OGD; c cytotoxicity by LDH measurement before and after OGD; d IF images of CD16 and CD206 staining in BV2 cells after OGD; semiquantitative results of e CD16 and f CD206 staining; g representative WB strips of CD16, IL-1 beta , CD206 and TGF-beta expression in microglia before and after OGD; quantitative WB results of h CD16, i IL-1 beta , j CD206 and k TGF-beta ; l N2a cell viabilities cocultured with BV2 cells before and after OGD; m schematic diagram of N2a and BV2 cell coculture; n representative WB stripes of synaptophysin and PSD95 expression in N2a coculture with BV2 cells before and after OGD; quantitative WB results of o synaptophysin and p PSD95 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37908012), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of human umbilical cord mesenchymal stem cell‐derived exosomes on the microglia cell polarization. (A and B) Western blot analysis and quantification of iNOS and COX‐2. (C and D) Western blot analysis and quantification of YM1 and ARG1. (E) Representative images of microglia bearing a pro‐inflammatory (M1) phenotype in each group were examined using anti‐CD86 (green) and anti‐Iba‐1 (red) antibodies, as well as DAPI (blue); scale bar = 25 μm. (F) Representative images of anti‐inflammatory phenotype microglia in each group were examined using anti‐CD206 (green), and anti‐Iba‐1 (red) antibodies, as well as DAPI (blue); scale bar = 25 μm. (G) Relative fluorescence intensity analysis for CD86 and CD206. (H) Representative images of primary microglia bearing a pro‐inflammatory phenotype in each group were examined using anti‐CD86 (green), anti‐Iba‐1 (red) antibodies, and DAPI (blue); scale bar = 25 μm. (I) Representative images of anti‐inflammatory phenotype primary microglia in each group were examined by using anti‐CD206 (green) and anti‐Iba‐1 (red) antibodies, as well as DAPI (blue); scale bar = 25 μm. (J) Relative fluorescence intensity analysis for CD86 and CD206 in primary microglia; n = 3; mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001, compared with the control group; #p < 0.05; ##p < 0.01; ###p < 0.001, compared with the LPS group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37697971), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2535U-100UG
Alexa Fluor 405	AF2535V-100UG
Alexa Fluor 488	FAB2535G
Alexa Fluor 532	AF2535X-100UG
Alexa Fluor 594	AF2535T-100UG
Alexa Fluor 647	AF2535R-100UG
Alexa Fluor 750	AF2535S-100UG
Alexa Fluor Plus 405	AF2535AFP405-100UG
Alexa Fluor Plus 488	AF2535AFP488-100UG
Alexa Fluor Plus 555	AF2535AFP555-100UG
Alexa Fluor Plus 594	AF2535AFP594-100UG
Alexa Fluor Plus 647	AF2535AFP647-100UG
Alexa Fluor Plus 680	AF2535AFP680-100UG
Allophycocyanin	FAB2535A
Biotin	BAF2535
CoraFluor 1	AF2535CL1
DyLight 350	AF2535D
DyLight 405	AF2535E
DyLight 488	AF2535K
DyLight 550	AF2535L
DyLight 594	AF2535M
DyLight 650	AF2535W
DyLight 680	AF2535Y
DyLight 755	AF2535Z
FITC	AF2535F
HRP	AF2535H
Janelia Fluor 525	AF2535JF525
Janelia Fluor 549	AF2535I
Janelia Fluor 646	AF2535J
Janelia Fluor 669	AF2535JF669
Phycoerythrin	FAB2535P
mFluor Violet 450 SE	AF2535MFV450
mFluor Violet 500 SE	AF2535MFV500
mFluor Violet 610 SE	AF2535MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2535P
Carrier Free	FAB2535A
Carrier Free	FAB2535G
Carrier Free	AF2535-SP

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AF2534

MMR/CD206/Mannose Receptor Antibody [Unconjugated]

(3 Reviews)

(43 Publications)

100 μg

AF2534

HAF019). A specific band was detected for MMR/CD206 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for MMR/CD206 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

MMR/CD206 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.2 mg/mL. A specific band was detected for MMR/CD206 at approximately 201 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Osteopontin is overexpressed in human MPM patients. (A) Real Time PCR for the Spp1 gene (osteopontin, OPN) in surgical human MPM samples. Comparison between tumor and undiseased adjacent tissues. Data are shown as mean ± SEM (Unpaired t test with Welch’s correction). (B) ELISA quantification of hOPN on plasma samples from 99 MPM patients and 101 healthy subjects. (ROUT, identify outlier and Unpaired t test with Welch’s correction). (C) Kaplan-Meier curves of overall survival according to OPN levels categorized based on CART analysis cut-off (n=61 MPM patients). (D–F) Representative images of immunohistochemistry in MPM tumor tissues stained for OPN or CD206 (40x, insert 100x) and semi-quantitative analysis in 28 cases (0=negative, 1 = 1-25% positivity, 2 = 26-50% positivity, 3= >50% positivity). Data are shown as mean ± SEM. (Unpaired t test with Welch’s correction). (G, H) ELISA quantification of hOPN on plasma samples from 18 MPM patients treated with immunotherapy. Blood was collected at baseline and after revalutation at 4-6 months. Patients with progressive disease (PD): 6 patients; stable disease (SD): 6 patients; partial response (PR): 6 patients. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37398648), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cisplatin does not affect TAM viability and M2 phenotype. (A) IHC analysis of CD68, stabilin-1 and CD206 expression in human breast cancer tissue treated and not treated with cisplatin-based therapy. (B) Quantitative analysis of CD68, stabilin-1 and CD206 expression. Tumor tissue was obtained after surgery for all patients. (C) Schematic description of ex vivo TAM generation which were stimulated with MCF-7 supernatants and treated by cisplatin. (D) Immunofluorescent staining/confocal microscopy of CD206 and stabilin-1 expression in CD68+ ex vivo TAMs. Scale bars are equal to 11µM. (E) Viability assay for ex vivo TAMs treated with cisplatin. Viability was estimated on day 6 before cisplatin treatment and day 9 after cisplatin-treatment (n=6 independent experiments). Data information: In (B), the level of protein expression is presented as Me (Q1;Q3). In (E), OD means defined in the percentage of NS control are presented as mean ± SD. The Manna-Whitney test was applied to compare two independent groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36960065), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2534U-100UG
Alexa Fluor 405	AF2534V-100UG
Alexa Fluor 488	AF2534G-100UG
Alexa Fluor 532	AF2534X-100UG
Alexa Fluor 594	AF2534T-100UG
Alexa Fluor 647	AF2534R-100UG
Alexa Fluor 700	AF2534N-100UG
Alexa Fluor 750	AF2534S-100UG
Alexa Fluor Plus 405	AF2534AFP405-100UG
Alexa Fluor Plus 488	AF2534AFP488-100UG
Alexa Fluor Plus 555	AF2534AFP555-100UG
Alexa Fluor Plus 594	AF2534AFP594-100UG
Alexa Fluor Plus 647	AF2534AFP647-100UG
Alexa Fluor Plus 680	AF2534AFP680-100UG
Allophycocyanin	AF2534A
Biotin	BAF2534
CoraFluor 1	AF2534CL1
DyLight 350	AF2534D
DyLight 405	AF2534E
DyLight 488	AF2534K
DyLight 550	AF2534L
DyLight 594	AF2534M
DyLight 650	AF2534W
DyLight 680	AF2534Y
DyLight 755	AF2534Z
FITC	AF2534F
HRP	AF2534H
Janelia Fluor 525	AF2534JF525
Janelia Fluor 549	AF2534I
Janelia Fluor 646	AF2534J
Janelia Fluor 669	AF2534JF669
PE	AF2534P
PerCP	AF2534C
mFluor Violet 450 SE	AF2534MFV450
mFluor Violet 500 SE	AF2534MFV500
mFluor Violet 610 SE	AF2534MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2534-SP

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NBP1-90020

MMR/CD206/Mannose Receptor Antibody - BSA Free

(1 Review)

(23 Publications)

0.1 ml

NBP1-90020

<b>Orthogonal Strategies Validation. </b>Analysis in human liver tissue.

Staining of human lung shows strong cytoplasmic positivity in macrophages.

Staining of human placenta shows strong cytoplasmic positivity in Hofbauer cells.

Staining of human cerebral cortex shows no cytoplasmic positivity in neurons as expected.

<b>Independent Antibodies Validation. </b>Staining of human cerebral cortex, liver, lung and placenta using antibody NBP1-90020 (A) shows similar protein distribution across tissues to independent antibody NBP2-33439 (B).

Staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.

<b>Orthogonal Strategies Validation. </b>Analysis in human lung and cerebral cortex tissues using NBP1-90020 antibody. Corresponding RNA-seq data are presented for the same tissues.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90020-25ul

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H00004360-M02

MMR/CD206/Mannose Receptor Antibody (5C11) - Azide and BSA Free

(24 Publications)

0.1 mg

H00004360-M02

ELISA: MMR/CD206/Mannose Receptor Antibody (5C11) [H00004360-M02] - Detection limit for recombinant GST tagged MRC1 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu, Fu, Pm

Species Glossary

Reactivity: Hu, Fu, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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DDX0380P-100

MMR/CD206/Mannose Receptor Antibody (122D2.08) - Azide and BSA Free

(2 Publications)

0.1 mg

DDX0380P-100

Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin

Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's

In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS

Reactivity:

Hu, Po, Bv, Sh

Species Glossary

Reactivity: Hu, Po, Bv, Sh

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB25341

MMR/CD206/Mannose Receptor Antibody (685645)

(10 Publications)

100 μg

MAB25341

HAF007). A specific band was detected for MMR/CD206 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for MMR/CD206 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMR/CD206 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMR/CD206 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25341) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB25341U-100UG
Alexa Fluor 405	FAB25341V-100UG
Alexa Fluor 488	FAB25341G-100UG
Alexa Fluor 532	FAB25341X-100UG
Alexa Fluor 594	FAB25341T-100UG
Alexa Fluor 647	FAB25341R-100UG
Alexa Fluor 700	FAB25341N-100UG
Alexa Fluor 750	FAB25341S-100UG
Alexa Fluor Plus 405	FAB25341AFP405-100UG
Alexa Fluor Plus 488	FAB25341AFP488-100UG
Alexa Fluor Plus 555	FAB25341AFP555-100UG
Alexa Fluor Plus 594	FAB25341AFP594-100UG
Alexa Fluor Plus 647	FAB25341AFP647-100UG
Alexa Fluor Plus 680	FAB25341AFP680-100UG
Allophycocyanin	FAB25341A
Biotin	FAB25341B
CoraFluor 1	FAB25341CL1
DyLight 350	FAB25341D
DyLight 405	FAB25341E
DyLight 488	FAB25341K
DyLight 550	FAB25341L
DyLight 594	FAB25341M
DyLight 650	FAB25341W
DyLight 680	FAB25341Y
DyLight 755	FAB25341Z
FITC	FAB25341F
HRP	FAB25341H
Janelia Fluor 525	FAB25341JF525
Janelia Fluor 549	FAB25341I
Janelia Fluor 646	FAB25341J
Janelia Fluor 669	FAB25341JF669
PE	FAB25341P
PerCP	FAB25341C
mFluor Violet 450 SE	FAB25341MFV450
mFluor Violet 500 SE	FAB25341MFV500
mFluor Violet 610 SE	FAB25341MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB25341-SP

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NBP2-52927

MMR/CD206/Mannose Receptor Antibody (CL0387) - BSA Free

(1 Review)

(2 Publications)

0.1 ml

NBP2-52927

Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human lung shows strong membranous positivity in macrophages.

Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human liver shows moderate positivity in kupffer cells.

Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human rectum shows moderate positivity in lymphoid cells.

Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (CL0387) [NBP2-52927] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43996AF350
Alexa Fluor 405	NBP3-43996AF405
Alexa Fluor 488	NBP3-43996AF488
Alexa Fluor 532	NBP3-43996AF532
Alexa Fluor 594	NBP3-43996AF594
Alexa Fluor 647	NBP3-43996AF647
Alexa Fluor 700	NBP3-43996AF700
Alexa Fluor 750	NBP3-43996AF750
Alexa Fluor Plus 405	NBP3-43996AFP405
Alexa Fluor Plus 488	NBP3-43996AFP488
Alexa Fluor Plus 555	NBP3-43996AFP555
Alexa Fluor Plus 594	NBP3-43996AFP594
Alexa Fluor Plus 647	NBP3-43996AFP647
Alexa Fluor Plus 680	NBP3-43996AFP680
Allophycocyanin	NBP3-43996APC
Biotin	NBP3-43996B
CoraFluor 1	NBP3-43996CL1
DyLight 350	NBP3-43996UV
DyLight 405	NBP3-43996V
DyLight 488	NBP3-43996G
DyLight 550	NBP3-43996R
DyLight 594	NBP3-43996DL594
DyLight 650	NBP3-43996C
DyLight 680	NBP3-43996FR
DyLight 755	NBP3-43996IR
FITC	NBP3-43996F
HRP	NBP3-43996H
Janelia Fluor 525	NBP3-43996JF525
Janelia Fluor 549	NBP3-43996JF549
Janelia Fluor 646	NBP3-43996JF646
Janelia Fluor 669	NBP3-43996JF669
PE	NBP3-43996PE
PerCP	NBP3-43996PCP
mFluor Violet 450 SE	NBP3-43996MFV450
mFluor Violet 500 SE	NBP3-43996MFV500
mFluor Violet 610 SE	NBP3-43996MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43996
BSA Free	NBP2-52927-25ul

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NBP2-66956

MMR/CD206/Mannose Receptor Antibody (JF0953)

100 ul

NBP2-66956

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of MMR/CD206/Mannose Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: D3 cell lysate

Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human lung tissue lysate Lane 2: HepG2 cell lysate

Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB2534

MMR/CD206/Mannose Receptor Antibody (309210) [Unconjugated]

(1 Review)

(6 Publications)

100 μg

MAB2534

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

MMR/CD206 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Rat Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB2534) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="MMR/CD206 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Rat Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB2534) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2534U-100UG
Alexa Fluor 405	FAB2534V-100UG
Alexa Fluor 488	FAB2534G-100UG
Alexa Fluor 532	FAB2534X-100UG
Alexa Fluor 594	FAB2534T-100UG
Alexa Fluor 647	FAB2534R-100UG
Alexa Fluor 700	FAB2534N-100UG
Alexa Fluor 750	FAB2534S-100UG
Alexa Fluor Plus 405	FAB2534AFP405-100UG
Alexa Fluor Plus 488	FAB2534AFP488-100UG
Alexa Fluor Plus 555	FAB2534AFP555-100UG
Alexa Fluor Plus 594	FAB2534AFP594-100UG
Alexa Fluor Plus 647	FAB2534AFP647-100UG
Alexa Fluor Plus 680	FAB2534AFP680-100UG
Allophycocyanin	FAB2534A
Biotin	FAB2534B
CoraFluor 1	FAB2534CL1
DyLight 350	FAB2534D
DyLight 405	FAB2534E
DyLight 488	FAB2534K
DyLight 550	FAB2534L
DyLight 594	FAB2534M
DyLight 650	FAB2534W
DyLight 680	FAB2534Y
DyLight 755	FAB2534Z
FITC	FAB2534F
HRP	FAB2534H
Janelia Fluor 525	FAB2534JF525
Janelia Fluor 549	FAB2534I
Janelia Fluor 646	FAB2534J
Janelia Fluor 669	FAB2534JF669
PE	FAB2534P
PerCP	FAB2534C
mFluor Violet 450 SE	FAB2534MFV450
mFluor Violet 500 SE	FAB2534MFV500
mFluor Violet 610 SE	FAB2534MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2534-SP

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NB600-1415

MMR/CD206/Mannose Receptor Antibody (15-2)

(2 Publications)

0.05 mg

NB600-1415

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB25342

MMR/CD206/Mannose Receptor Antibody (685641)

(2 Publications)

100 μg

MAB25342

HAF007). A specific band was detected for MMR/CD206 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human immature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for MMR/CD206 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human immature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMR/CD206 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

Human monocyte-derived immature dendritic cells were stained with Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342, filled histogram) or isotype control antibody (Catalog # <a class=

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB25342U-100UG
Alexa Fluor 405	FAB25342V-100UG
Alexa Fluor 488	FAB25342G
Alexa Fluor 532	FAB25342X
Alexa Fluor 594	FAB25342T-100UG
Alexa Fluor 647	FAB25342R-100UG
Alexa Fluor 700	FAB25342N-100UG
Alexa Fluor 750	FAB25342S-100UG
Alexa Fluor Plus 405	FAB25342AFP405-100UG
Alexa Fluor Plus 488	FAB25342AFP488-100UG
Alexa Fluor Plus 555	FAB25342AFP555-100UG
Alexa Fluor Plus 594	FAB25342AFP594-100UG
Alexa Fluor Plus 647	FAB25342AFP647-100UG
Alexa Fluor Plus 680	FAB25342AFP680-100UG
Allophycocyanin	FAB25342A
Allophycocyanin/Cy7	FAB25342APCCY7
Biotin	FAB25342B
CoraFluor 1	FAB25342CL1
DyLight 350	FAB25342D
DyLight 405	FAB25342E
DyLight 488	FAB25342K
DyLight 550	FAB25342L
DyLight 594	FAB25342M
DyLight 650	FAB25342W
DyLight 680	FAB25342Y
DyLight 755	FAB25342Z
FITC	FAB25342F
HRP	FAB25342H
Janelia Fluor 525	FAB25342JF525
Janelia Fluor 549	FAB25342I
Janelia Fluor 646	FAB25342J
Janelia Fluor 669	FAB25342JF669
PE/Atto594	FAB25342PEATT594
PE/Cy5.5	FAB25342PECY55
PE/Cy7	FAB25342PECY7
PerCP	FAB25342C
Phycoerythrin	FAB25342P
mFluor Violet 450 SE	FAB25342MFV450
mFluor Violet 500 SE	FAB25342MFV500
mFluor Violet 610 SE	FAB25342MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB25342P
Carrier Free	FAB25342A
Carrier Free	FAB25342G
Carrier Free	MAB25342-SP
Carrier Free	FAB25342V-100UG
Carrier Free	FAB25342U-100UG
Carrier Free	FAB25342T-100UG
Carrier Free	FAB25342S-100UG
Carrier Free	FAB25342R-100UG
Carrier Free	FAB25342N-100UG

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NBP3-09101

MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin

0.1 mg

NBP3-09101

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

MMR/CD206/Mannose Receptor Antibodies

MMR/CD206/Mannose Receptor Antibody [Unconjugated]

MMR/CD206/Mannose Receptor Antibody [Unconjugated]

MMR/CD206/Mannose Receptor Antibody - BSA Free

MMR/CD206/Mannose Receptor Antibody (5C11) - Azide and BSA Free

MMR/CD206/Mannose Receptor Antibody (122D2.08) - Azide and BSA Free

MMR/CD206/Mannose Receptor Antibody (685645)

MMR/CD206/Mannose Receptor Antibody (CL0387) - BSA Free

MMR/CD206/Mannose Receptor Antibody (JF0953)

MMR/CD206/Mannose Receptor Antibody (309210) [Unconjugated]

MMR/CD206/Mannose Receptor Antibody (15-2)

MMR/CD206/Mannose Receptor Antibody (685641)

MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin