MMP-12 Antibody (MM0025-7G20)

Product Discontinued

MMP-12 Antibody (MM0025-7G20) Summary

• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant human MMP-12).
• Specificity: MMP12 (MM0025-7G20)
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP12
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: This antibody recognizes human MMP-12 in western blot without cross reactivity with other human MMPs.

Reactivity Notes

This antibody is reactive against human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for MMP-12 Antibody (MM0025-7G20)

• EC 3.4.24
• hME
• HMEEC 3.4.24.65
• Macrophage elastase
• macrophage metalloelastase
• matrix metallopeptidase 12 (macrophage elastase)
• matrix metalloproteinase 12 (macrophage elastase)
• Matrix metalloproteinase-12
• ME
• MGC138506
• MME
• MMP12
• MMP-12

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 was first described in murine macrophages, later in human macrophages, and more recently in other cell types. Also known as metalloelastase, MMP12 is able to degrade elastin, entactin, laminin 1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP12 is often confused with the Serine proteinase, Leukocyte elastase (EC 3.4.21.37) because of similar nomenclature. MMP12 is structurally similar to the classical MMPs (MMP1, MMP3); it contains a propeptide with autoinhibitory cysteine switch site, a well-conserved zinc site, hinge region and hemopexin domain. MMP12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kD, and is quickly activated to the 45 kD form; and this breaks down to cascade of active forms, ending with the common 22 kD form. Stimulated macrophages produce MMP12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP12
• Entrez:
  • 4321(Human)