DMPK Antibody

Immunohistochemistry-Paraffin: DMPK Antibody [NBP1-85010] - Analysis in human skeletal muscle and liver tissues using NBP1-85010 antibody. Corresponding DMPK RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: DMPK Antibody [NBP1-85010] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: DMPK Antibody [NBP1-85010] - Staining of human tonsil shows very weak cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: DMPK Antibody [NBP1-85010] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: DMPK Antibody [NBP1-85010] - Staining of human skeletal muscle shows strong cytoplasmic in myocytes.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DMPK Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LGVFAYEMFYGQTPFYADSTAETYGKIVHYKEHLSLPLVDEGVPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DMPK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DMPK Antibody

• DM1
• DM1PK
• DMK
• DMKEC 2.7.11.1
• DM-kinase
• DMmyotonin protein kinase A
• DMPK
• dystrophia myotonica-protein kinase
• MDPK
• MDPKDM protein kinase
• MT-PK
• MT-PKmyotonic dystrophy associated protein kinase
• Myotonic dystrophy protein kinase
• myotonin-protein kinase
• thymopoietin homolog

Background

Dystrophia myotonica protein kinase is encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DMPK