AFF4 Antibody (2E12)

Western Blot: AFF4 Antibody (2E12) [H00027125-M01] - Analysis of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody (M01), clone 2E12.Lane 1: AFF4 transfected lysate( 40.2 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: AFF4 Antibody (2E12) [H00027125-M01] - Analysis of monoclonal antibody to AFF4 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.

Sandwich ELISA: AFF4 Antibody (2E12) [H00027125-M01] - Detection limit for recombinant GST tagged AFF4 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

AFF4 Antibody (2E12) Summary

• Immunogen: AFF4 (NP_055238, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKW
• Specificity: AFF4 - AF4/FMR2 family, member 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFF4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AFF4 Antibody (2E12)

• AF4/FMR2 family, member 4
• AF5Q31AF4/FMR2 family member 4
• ALL1-fused gene from chromosome 5q31 protein
• Major CDK9 elongation factor-associated protein
• MCEFALL1 fused gene from 5q31
• MGC75036
• Protein AF-5q31

Background

AFF4, or AF4/FMR2 family member 4, contains a 127 kDa, 98 kDa, and 39 kDa isoform, and is involved in the process of improving the catalytic rate of RNA polymerase II transcription. Disease research is currently being conducted with AFF4 on its relation to acute lymphoblastic leukemia, as well as other types of leukemia. This protein interacts with SIAH1, CCNT1, MLLT3, MLLT1, and MED10 during the regulation of DNA-dependent transcription and the transcription of RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFF4
• Entrez:
  • 27125(Human)
• OMIM:
  • 604417(Human)
• Uniprot:
  • NP_055238(Human)