SMARCA1 Antibody (2H7B8) - BSA Free

Western Blot: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis using SMARCA1 mAb against HEK293 (1) and SMARCA1 (AA: 933-1070)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of paraffin-embedded rectum cancer tissues using SMARCA1 mouse mAb with DAB staining.

Flow Cytometry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of NIH/3T3 cells using SMARCA1 mouse mAb (green) and negative control (red).

Western Blot: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis using SMARCA1 mAb against human SMARCA1 (AA: 933-1070) recombinant protein. (Expected MW is 42.4 kDa)

Western Blot: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis using SMARCA1 mouse mAb against SW620 (1) and HT-29 (2) cell lysate.

Immunohistochemistry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of paraffin-embedded esophageal cancer tissues using SMARCA1 mouse mAb with DAB staining.

ELISA: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, IHC-P
• Clone: 2H7B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SMARCA1 Antibody (2H7B8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human SMARCA1 (AA: 933-1070) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMARCA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 122.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SMARCA1 Antibody (2H7B8) - BSA Free

• ATP-dependent helicase SMARCA1
• DKFZp686D1623
• EC 3.6.1
• EC 3.6.4.-
• FLJ41547
• global transcription activator homologous sequence
• Nucleosome-remodeling factor subunit SNF2L
• NURF140
• probable global transcription activator SNF2L1
• SNF2L
• SNF2L1ISWI
• SNF2LB
• SNF2-like 1
• SNF2LT
• subfamily a, member 1
• sucrose nonfermenting 2-like protein 1
• SWI
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 1
• SWI2

Background

This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCA1