AF4 Antibody

Images

 
Independent Antibodies: Western Blot: AF4 Antibody [NBP1-28728] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- AF4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

AF4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AFF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
Application Notes
Not recommended for immunoprecipitation. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NBP1-28728.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AF4 Antibody

  • AF-4
  • AF4/FMR2 family member 1
  • AF4/FMR2 family, member 1
  • AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2
  • ALL1-fused gene from chromosome 4 protein
  • FEL
  • MLLT2MGC134969
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2
  • PBM1
  • pre-B-cell monocytic leukemia partner 1
  • Protein AF-4
  • Protein FEL
  • Proto-oncogene AF4

Background

AF4 is a member of the AF4/LAF4/FMR2 (ALF) family of transcription cofactors. It has been identified as part of a translocation [t(4:11)(q21;q23)] found in patients with acute leukemia involving MLL/HRX. AF4 functions as a cofactor of transcriptional elongation and chromatin remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AF4 Antibody (NBP1-28728)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Array NBP1-28728

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol AFF1
Entrez
Uniprot