DOT1L Antibody

Images

 
Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. ...read more
Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution ...read more
Independent Antibodies: Western Blot: DOT1L Antibody [NB100-40845] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DOT1L antibody ...read more
Western Blot: DOT1L Antibody [NB100-40845] - siRNA mediated gene silencing of DOT1L validated by Q-PCR and western blot. The results show a down regulation of DOT1L mRNA expression. Empty bars are untreated cells and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

DOT1L Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DOT1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
  • Knockdown Validated
Application Notes
Chromatin Immunoprecipitation was reported in scientific literature (PMID:19734945). For ChIP-Seq: 4 ug/30 ug chromatin. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DOT1L Overexpression Lysate
DOT1L Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-40845 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23861670).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DOT1L Antibody

  • DOT1L
  • DOT1-like protein
  • DOT1-like, histone H3 methyltransferase (S. cerevisiae)
  • EC 2.1.1.43
  • H3-K79-HMTase
  • KIAA1814DKFZp586P1823
  • KMT4
  • KMT4H3 lysine-79 specific
  • Lysine N-methyltransferase 4

Background

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DOT1L Antibody (NB100-40845)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, IHC, KD, WB.


Filter By Application
ChIP
(1)
IHC
(2)
KD
(1)
WB
(4)
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Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-40845 Applications Species
Luo H, Zhu G, Xu J et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice Cancer Cell Nov 27 2019 [PMID: 31786140] (Mouse) Mouse
Lv L, Li Q, Chen S et al. miR-133b suppresses colorectal cancer cell stemness and chemoresistance by targeting methyltransferase DOT1L Exp. Cell Res. [PMID: 31525340] (WB) WB
Liu B, Yang L, Zhu X et al. Yeats4 drives ILC lineage commitment via activation of Lmo4 transcription J. Exp. Med. Aug 21 2019 [PMID: 31434684] (WB, Human) WB Human
Szablewski V, Bret C, Kassambara A et al. An epigenetic regulator-related score (EpiScore) predicts survival in patients with diffuse large B cell lymphoma and identifies patients who may benefit from epigenetic therapy Oncotarget Apr 10 2018 [PMID: 29721185] (IHC, Human) IHC Human
Loeser Heike, Waldschmidt Dirk, Kuetting Fabian et al. Copy-number variation and protein expression of DOT1L in pancreatic adenocarcinoma as a potential drug target. Molecular and Clinical Oncology 2017 Mar 14 [PMID: 28529740] (IHC, Human) IHC Human
Lakhdar R, Drost EM, MacNee W et al. 2D-DIGE proteomic analysis of vastus lateralis from COPD patients with low and normal fat free mass index and healthy controls Respir Res. 2017 May 02 [PMID: 28468631] (WB, KD, Human) WB, KD Human
Gilan O, Lam EY, Becher I et al. Functional interdependence of BRD4 and DOT1L in MLL leukemia. Nat. Struct. Mol. Biol. Jun 13 2016 [PMID: 27294782]
Oksenych V, Zhovmer A, Ziani S et al. Histone Methyltransferase DOT1L Drives Recovery of Gene Expression after a Genotoxic Attack. PLoS Genet 2013 Jul [PMID: 23861670] (WB, Mouse) WB Mouse
Shah S, Henriksen MA. A novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression. J Biol Chem 2011 Dec [PMID: 22002246]
Jacinto FV, Ballestar E, Esteller M. Impaired recruitment of the histone methyltransferase DOT1L contributes to the incomplete reactivation of tumor suppressor genes upon DNA demethylation. Oncogene 2009 Nov 26 [PMID: 19734945] (ChIP, Human) ChIP Human
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for DOT1L Antibody (NB100-40845) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol DOT1L
Entrez
Uniprot