DOT1L Antibody


Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. more
Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution more
Independent Antibodies: Western Blot: DOT1L Antibody [NB100-40845] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DOT1L antibody more
Western Blot: DOT1L Antibody [NB100-40845] - siRNA mediated gene silencing of DOT1L validated by Q-PCR and western blot. The results show a down regulation of DOT1L mRNA expression. Empty bars are untreated cells and more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ, KD
0.2 mg/ml
Validated by:

Independent Antibodies


Order Details

DOT1L Antibody Summary

The immunogen recognized by this antibody maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation Sequencing
  • Chromatin Immunoprecipitation
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
Application Notes
Chromatin Immunoprecipitation was reported in scientific literature (PMID:19734945). For ChIP-Seq: 4 ug/30 ug chromatin. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
DOT1L Overexpression Lysate
Read Publications using
NB100-40845 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23861670).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
0.2 mg/ml
Immunogen affinity purified

Alternate Names for DOT1L Antibody

  • DOT1L
  • DOT1-like protein
  • DOT1-like, histone H3 methyltransferase (S. cerevisiae)
  • EC
  • H3-K79-HMTase
  • KIAA1814DKFZp586P1823
  • KMT4
  • KMT4H3 lysine-79 specific
  • Lysine N-methyltransferase 4


DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: CHIP-SEQ, ChIP, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Species: Ce
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Species: Hu, Mu
Applications: ChIP, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Species: Hu
Applications: IHC, IHC-P, IP (-), WB
Species: Hu
Applications: WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ICC, WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ, KD

Publications for DOT1L Antibody (NB100-40845)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: CHIP-SEQ, ChIP, ICC/IF, IHC, KD, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-40845 Applications Species
Yang M, Yu H, Yu X et al. Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells Cell stem cell Mar 3 2022 [PMID: 35143761] (ICC/IF, CHIP-SEQ, Mouse) ICC/IF, CHIP-SEQ Mouse
Luo H, Zhu G, Xu J et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice Cancer Cell Nov 27 2019 [PMID: 31786140] (Mouse) Mouse
Lv L, Li Q, Chen S et al. miR-133b suppresses colorectal cancer cell stemness and chemoresistance by targeting methyltransferase DOT1L Exp. Cell Res. [PMID: 31525340] (WB) WB
Liu B, Yang L, Zhu X et al. Yeats4 drives ILC lineage commitment via activation of Lmo4 transcription J. Exp. Med. Aug 21 2019 [PMID: 31434684] (WB, Human) WB Human
Szablewski V, Bret C, Kassambara A et al. An epigenetic regulator-related score (EpiScore) predicts survival in patients with diffuse large B cell lymphoma and identifies patients who may benefit from epigenetic therapy Oncotarget Apr 10 2018 [PMID: 29721185] (IHC, Human) IHC Human
Loeser Heike, Waldschmidt Dirk, Kuetting Fabian et al. Copy-number variation and protein expression of DOT1L in pancreatic adenocarcinoma as a potential drug target. Molecular and Clinical Oncology 2017 Mar 14 [PMID: 28529740] (IHC, Human) IHC Human
Lakhdar R, Drost EM, MacNee W et al. 2D-DIGE proteomic analysis of vastus lateralis from COPD patients with low and normal fat free mass index and healthy controls Respir Res. 2017 May 02 [PMID: 28468631] (WB, KD, Human) WB, KD Human
Gilan O, Lam EY, Becher I et al. Functional interdependence of BRD4 and DOT1L in MLL leukemia. Nat. Struct. Mol. Biol. Jun 13 2016 [PMID: 27294782]
Oksenych V, Zhovmer A, Ziani S et al. Histone Methyltransferase DOT1L Drives Recovery of Gene Expression after a Genotoxic Attack. PLoS Genet 2013 Jul [PMID: 23861670] (WB, Mouse) WB Mouse
Shah S, Henriksen MA. A novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression. J Biol Chem 2011 Dec [PMID: 22002246]
Show All 12 Publications.

Reviews for DOT1L Antibody (NB100-40845) (0)

There are no reviews for DOT1L Antibody (NB100-40845). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for DOT1L Antibody (NB100-40845) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional DOT1L Products

Bioinformatics Tool for DOT1L Antibody (NB100-40845)

Discover related pathways, diseases and genes to DOT1L Antibody (NB100-40845). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DOT1L Antibody (NB100-40845)

Discover more about diseases related to DOT1L Antibody (NB100-40845).

Pathways for DOT1L Antibody (NB100-40845)

View related products by pathway.

PTMs for DOT1L Antibody (NB100-40845)

Learn more about PTMs related to DOT1L Antibody (NB100-40845).

Research Areas for DOT1L Antibody (NB100-40845)

Find related products by research area.

Blogs on DOT1L

There are no specific blogs for DOT1L, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our DOT1L Antibody and receive a gift card or discount.


Gene Symbol DOT1L