Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. ...read more
Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution ...read more
Independent Antibodies: Western Blot: DOT1L Antibody [NB100-40845] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DOT1L antibody ...read more
Western Blot: DOT1L Antibody [NB100-40845] - siRNA mediated gene silencing of DOT1L validated by Q-PCR and western blot. The results show a down regulation of DOT1L mRNA expression. Empty bars are untreated cells and ...read more
Chromatin Immunoprecipitation was reported in scientific literature (PMID:19734945). For ChIP-Seq: 4 ug/30 ug chromatin. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
185 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 23861670).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for DOT1L Antibody
DOT1-like, histone H3 methyltransferase (S. cerevisiae)
KMT4H3 lysine-79 specific
Lysine N-methyltransferase 4
DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.