Mineralocorticoid R/NR3C2 Antibody (385707)

Western blot shows lysates of LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Mineralocorticoid R/NR3C2 Monoclonal Antibody (Catalog # MAB4369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 385707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Mineralocorticoid R/NR3C2 Antibody (385707) Summary

• Immunogen: E. coli-derived recombinant human Mineralocorticoid R/NR3C2
  Met1-Leu670
  Accession # AAI11759
• Specificity: Detects human Mineralocorticoid R/NR3C2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mineralocorticoid R/NR3C2 Antibody (385707)

• FLJ41052
• MGC133092
• Mineralocorticoid R
• mineralocorticoid receptor 1
• mineralocorticoid receptor delta
• MineralocorticoidR
• MLR
• MLRmineralocorticoid receptor
• MRMCRaldosterone receptor
• NR3C2
• NR3C2VIT
• Nuclear receptor subfamily 3 group C member 2
• nuclear receptor subfamily 3, group C, member 2

Background

Mineralocorticoid Receptor (MR) is a member of the nuclear hormone receptor family. Upon binding of ligand (aldosterone or corticosterone) MR translocates to the nucleus and can form either homo or heterodimers. MR dimers bind to the mineralocorticoid response element (AGAACANNNTGTTCT) to regulate gene transcription. There are four known isoforms of MR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mineralocorticoid R/NR3C2 Antibody (MAB4369)(1)

Publication using MAB4369

• Sojka, C;Wang, HV;Bhatia, TN;Li, Y;Chopra, P;Sing, A;Voss, A;King, A;Wang, F;Joseph, K;Ravi, VM;Olson, J;Hoang, K;Nduom, E;Corces, VG;Yao, B;Sloan, SA; Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma Nature cell biology 2025-01-08 [PMID: 39779941] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: NR3C2
• Uniprot:
  • AAI11759()