Mimecan/OIF Antibody [Unconjugated]

Images

 
Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by HRP-conjugated Anti-Goat ...read more
Simple Western lane view shows lysates of human heart (aorta) tissue, loaded at 0.2 mg/mL. A specific band was detected for Mimecan at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Mimecan ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Mimecan/OIF Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mimecan
Ala20-Phe298
Accession # P20774
Specificity
Detects human Mimecan in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Mimecan is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OGN
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
Publications
Read Publication using
AF2660 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mimecan/OIF Antibody [Unconjugated]

  • DKFZp586P2421
  • KSPG25
  • mimecan proteoglycan
  • Mimecan
  • OGN
  • OIF
  • OIFOG
  • osteoglycin (osteoinductive factor)
  • osteoglycin
  • Osteoinductive factor
  • SLRR3A
  • SLRR3Acorneal keratan sulfate proteoglycan

Background

Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine‑rich repeats in the central domain, which is flanked by N- and C-terminal cysteine-rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C-terminal amino acid residues) and Mimecan (containing 223 most C-terminal residues). Human Mimecan shares approximately 85% amino acid sequence homology with the mouse or rat Mimecan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mimecan/OIF Antibody (AF2660)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Bioinformatics

Gene Symbol OGN
Uniprot