Mimecan/OIF Antibody [Unconjugated]

Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mimecan at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human heart (aorta) tissue, loaded at 0.2 mg/mL. A specific band was detected for Mimecan at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mimecan/OIF Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Mimecan
  Ala20-Phe298
  Accession # P20774
• Specificity: Detects human Mimecan in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Mimecan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OGN

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mimecan/OIF Antibody [Unconjugated]

• DKFZp586P2421
• KSPG25
• mimecan proteoglycan
• Mimecan
• OGN
• OIF
• OIFOG
• osteoglycin (osteoinductive factor)
• osteoglycin
• Osteoinductive factor
• SLRR3A
• SLRR3Acorneal keratan sulfate proteoglycan

Background

Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine‑rich repeats in the central domain, which is flanked by N- and C-terminal cysteine-rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C-terminal amino acid residues) and Mimecan (containing 223 most C-terminal residues). Human Mimecan shares approximately 85% amino acid sequence homology with the mouse or rat Mimecan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mimecan/OIF Antibody (AF2660)(1)

Publication using AF2660

• X Hu, YQ Li, QG Li, YL Ma, JJ Peng, SJ Cai Osteoglycin-induced VEGF Inhibition Enhances T Lymphocytes Infiltrating in Colorectal Cancer EBioMedicine, 2018;0(0):. 2018 [PMID: 30037719] (Western Blot, Human)

Bioinformatics

• Gene Symbol: OGN
• Uniprot:
  • P20774()