Asporin Antibody

Western Blot: Asporin Antibody [NB100-1514] - (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Asporin Antibody [NB100-1514] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear membrane staining. The nuclear stain is DA

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Asporin Antibody Summary

• Immunogen: Peptide with sequence C-IHENKVKKIQKDT corresponding to internal region according to NP_060150.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ASPN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA detection limit 1:64000
  • Western Blot 0.1-1 ug/ml
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Asporin Peptide (NB100-1514PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Asporin Antibody

• asporin (LRR class 1)
• asporin
• FLJ20129
• OS3
• periodontal ligament associated protein 1
• Periodontal ligament-associated protein 1
• PLAP-1
• PLAP1SLRR1Casporin proteoglycan
• small leucine-rich protein 1C

Background

ASPN, or Asporin, belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin. Asporin is expressed in a variety of human tissues with higher levels in osteoarthritic articular cartilage, aorta, uterus, heart and liver. The function of Asporin is unclear, but available data indicates a collagen binding capacity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Asporin Antibody (NB100-1514)(4)

Publications using NB100-1514

• Medzikovic L, Aryan L, Ruffenach G et al. Myocardial fibrosis and calcification are attenuated by microRNA-129-5p targeting Asporin and Sox9 in cardiac fibroblasts JCI insight 2023-05-08 [PMID: 37154157] (IHC, Human)
• Bala K, Cuellar A, Herren AW, Boyadjiev SA Identification of differentially expressed proteins between fused and open sutures in sagittal nonsyndromic craniosynostosis during suture development by quantitative proteomic analysis Proteomics. Clinical applications 2021-02-13 [PMID: 33580899]
• Yang L, Nyalwidhe JO, Guo S et al. Targeted identification of metastasis-associated cell-surface sialoglycoproteins in prostate cancer. Mol Cell Proteomics. 2011-06-01 [PMID: 21447706]
• Kizawa H, Kou I, Iida A et al. An aspartic acid repeat polymorphism in asporin inhibits chondrogenesis and increases susceptibility to osteoarthritis. Nat Genet 2005-02-01 [PMID: 15640800]

Bioinformatics

• Gene Symbol: ASPN
• Entrez:
  • 54829(Human)
• Uniprot:
  • Q9BXN1(Human)