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Asporin Antibody

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Western Blot: Asporin Antibody [NB100-1514] - (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.
Western Blot: Asporin Antibody [NB100-1514] - (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) ...read more
Immunocytochemistry/ Immunofluorescence: Asporin Antibody [NB100-1514] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Asporin Antibody Summary

Immunogen
Peptide with sequence C-IHENKVKKIQKDT corresponding to internal region according to NP_060150.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ASPN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1-1 ug/ml
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Peptide ELISA detection limit 1:64000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Asporin Peptide (NB100-1514PEP)
Publications
Read Publications using NB100-1514.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Asporin Antibody

  • asporin (LRR class 1)
  • asporin
  • FLJ20129
  • OS3
  • periodontal ligament associated protein 1
  • Periodontal ligament-associated protein 1
  • PLAP-1
  • PLAP1SLRR1Casporin proteoglycan
  • small leucine-rich protein 1C

Background

ASPN, or Asporin, belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin. Asporin is expressed in a variety of human tissues with higher levels in osteoarthritic articular cartilage, aorta, uterus, heart and liver. The function of Asporin is unclear, but available data indicates a collagen binding capacity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Asporin Antibody
NB100-1514
Asporin Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, PEP-ELISA

Publications for Asporin Antibody (NB100-1514)(2)


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Bioinformatics

Gene Symbol ASPN
Entrez
Uniprot