Recombinant Human MIF His Avi-tag Protein [Biotin]

SDS-Page: Recombinant Human MIF His Avi-tag Protein [Biotin] [NBP3-18522] - Western blot under reducing and non-reducing conditions

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Conjugate: Biotin
• Concentration: LYOPH

Recombinant Human MIF His Avi-tag Protein [Biotin] Summary

• Description: A single, non-glycosylated, biotinylated polypeptide chain containing 136 amino acids corresponding to human MIF, with 6x His and Avi tags at the C-terminus. Source: E. coli

  Amino Acid Sequence: MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFAHHHHH HGLNDIFEAQ KIEWHE

• Details of Functionality: Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MIF
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug, as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0, 0.02 % Tween-20.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Alternate Names for Recombinant Human MIF His Avi-tag Protein [Biotin]

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MIF