Western Blot: MIF Antibody [NB100-1789] - Analysis of MIF in Human Thymus lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA: MIF Antibody [NB100-1789] - (1.5ug/ml) as the reporter used with capture rabbit antibody (5ug/ml)
EIA: Sandwich ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. WB: Approx. 13 kDa band observed in human lymph node, and thymus lysates and similar bands are observed in Daudi and Jurkat lysates (calculated MW of 12.5 kDa band according to NP_002406.1).
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.