MIF Antibody

Images

 
Western Blot: MIF Antibody [NB100-1789] - Analysis of MIF in Human Thymus lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA: MIF Antibody [NB100-1789] - (1.5ug/ml) as the reporter used with capture rabbit antibody (5ug/ml)

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, EIA, ELISA, PEP-ELISA, S-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MIF Antibody Summary

Immunogen
Peptide with sequence C-NAANVGWNNSTFA corresponding to C-Terminus according to NP_002406.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MIF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • ELISA 1:100 - 1:2000
  • Enzyme Immunoassay 1.5 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Sandwich ELISA 1.5 - 3 ug/ml
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. WB: Approx. 13 kDa band observed in human lymph node, and thymus lysates and similar bands are observed in Daudi and Jurkat lysates (calculated MW of 12.5 kDa band according to NP_002406.1).
Positive Control
Tonsil Lysate (NB820-59272)
MIF Lysate (NBL1-13108)
Thymus Lysate (NB820-59269)
Lymph Node Lysate (NB820-59242)
Publications
Read Publications using NB100-1789.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse. reactivity reported in scientific literature (PMID: 19066630).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MIF Antibody

  • EC 5.3.2.1
  • EC 5.3.3.12
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1985
Species: Hu, Mu(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-89772
Species: Hu
Applications: IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-1789
Species: Hu, Rt
Applications: WB, EIA, ELISA, PEP-ELISA, S-ELISA

Publications for MIF Antibody (NB100-1789)(3)

Reviews for MIF Antibody (NB100-1789) (0)

There are no reviews for MIF Antibody (NB100-1789). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MIF Antibody (NB100-1789) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MIF Products

Bioinformatics Tool for MIF Antibody (NB100-1789)

Discover related pathways, diseases and genes to MIF Antibody (NB100-1789). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MIF Antibody (NB100-1789)

Discover more about diseases related to MIF Antibody (NB100-1789).
 

Pathways for MIF Antibody (NB100-1789)

View related products by pathway.

PTMs for MIF Antibody (NB100-1789)

Learn more about PTMs related to MIF Antibody (NB100-1789).
 

Research Areas for MIF Antibody (NB100-1789)

Find related products by research area.

Blogs on MIF

There are no specific blogs for MIF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MIF Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MIF
Entrez
Uniprot