MIF Antibody (BLR141J)

Western Blot;MIF Antibody (BLR141J) Whole cell lysate (25 µg) from HEK293T, HeLa, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MIF recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: BLR141J
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MIF Antibody (BLR141J) Summary

• Description: Novus Biologicals Rabbit MIF Antibody (BLR141J) (NBP3-14721) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: residues 2-115 (full length)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MIF
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for MIF Antibody (BLR141J)

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MIF