mGluR5 Antibody - BSA Free

Western Blot: mGluR5 Antibody [NBP3-13190] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody (NBP3-13190) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: mGluR5 Antibody [NBP3-13190] - mGluR5 antibody detects mGluR5 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mGluR5 stained by mGluR5 antibody (NBP3-13190) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

mGluR5 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of mouse mGluR5. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Reactivity Notes

Human (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for mGluR5 Antibody - BSA Free

• glutamate receptor, metabotropic 5
• GPRC1E
• GPRC1EMGLUR5metabotropic glutamate receptor 5
• GRM5
• mGlu5
• mGluR5

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Class I metabotropic glutamate receptors (mGluRs) have been postulated to play a role in synaptic plasticity. It has been suggested that mGluR5 may be the primary high affinity ACPD receptor in CA1 neurons (1). Also, neuroprotection by mGluR system has been linked to the modulation of both the free radical nitric oxide (NO) and programmed cell death (PCD). Activation of group I mGluR subtypes utilized an effective, "upstream" mechanism for the inhibition of cysteine protease activity that offered an enhanced level of neuroprotection through both the preservation of genomic DNA integrity and the maintenance of PS membrane asymmetry (2). It has also been shown that activation of mGluRs enhances formation of an mGluR-Homer-PIKE-L complex, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Our findings indicate that this complex mediates the well-known ability of agonists of mGluR5 to prevent neuronal apoptosis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRM5