mGluR1 Antibody

Western Blot: mGluR1 Antibody [NB110-39033] - WB detection of GluR1 in 20 ug of human brain lysate (lane 2) with GluR1 polyclonal antibody at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. Lane 1 represents the MW marker.

Immunocytochemistry/Immunofluorescence: mGluR1 Antibody [NB110-39033] - Primary Rat Cortical Neurons, image courtesy of customer.

Immunohistochemistry-Frozen: mGluR1 Antibody [NB110-39033] - Immunofluorescence labeling for GluR1 in rat brain (cingulate cortex). GluR1 detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color). Working dilution is 1:100-1:500.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

mGluR1 Antibody Summary

• Immunogen: Synthetic peptide comprising residues 1159-1171 [SSVPSSPVSESVL] of the human mGluR1 protein.
• Localization: Cell membrane
• Specificity: Glutamate Receptor
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: This metabotropic Glutamate Receptor 1a antibody is useful for Western Blot, Immunohistochemistry-Frozen and Immunohistochemistry-paraffin applications. Use in Immunocytochemistry/immunofluorescence was reported in scientific literature (PMID: 19688350) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 132.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NMDAR1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for mGluR1 Antibody

• glutamate receptor, metabotropic 1
• GPRC1A
• GRM1
• GRM1A
• metabotropic glutamate receptor 1
• mGluR1
• MGLUR1A
• MGLUR1GPRC1AmGlu1

Background

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GRIA1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mGluR1 Antibody (NB110-39033)(2)

Publications using NB110-39033

• Tang Y, Liu L, Xu D et al. Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model Brain Behav. Immun. 2017 Oct 31 [PMID: 29080683] (Rat)
• Hirdes W, Napp N, Wulfsen I et al. Erg K+ currents modulate excitability in mouse mitral/tufted neurons. Pflugers Arch 2009 Nov [PMID: 19688350] (ICC/IF, Mouse)

Review for mGluR1 Antibody (NB110-39033) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Janosch Heller ICC Rat 03/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary rat cortical neurons
• Species: Rat

Comments

• Comments: Fixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT
  Quenching: 0.1% NaBH4 in PBS, 7 minutes RT
  Blocking: 3% BSA in PBS, 30 minutes RT
  Permeabilization: 0.1% Triton-X100, 10 minutes RT
  Primary Antibody: 1:500 in 3% BSA in PBS, overnight, 4°C
  Wash: three times wash with PBS, 10 min each
  Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:1800 in PBS with 1% BSA, 2 hours, RT
  Wash: three times wash with PBS, 10 min each
  Microscope: Bruker Vutara 350
  
  The antibody labels clusters of metabotropic receptors along the whole cell.

Bioinformatics

• Gene Symbol: GRM1
• Entrez:
  • 2911(Human)
  • 14810(Mouse)
  • 24408(Rat)
• Uniprot:
  • Q13255()