mGluR1 Antibody - BSA Free

Images

 
Western Blot: mGluR1 Antibody [NB110-39033] - WB detection of GluR1 in 20 ug of human brain lysate (lane 2) with GluR1 polyclonal antibody at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. Lane ...read more
Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody [NB110-39033] - Primary Rat Cortical Neurons, image courtesy of customer.
Immunohistochemistry-Frozen: mGluR1 Antibody [NB110-39033] - Immunofluorescence labeling for GluR1 in rat brain (cingulate cortex). GluR1 detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

mGluR1 Antibody - BSA Free Summary

Immunogen
Synthetic peptide comprising residues 1159-1171 [SSVPSSPVSESVL] of the human mGluR1 protein.
Localization
Cell membrane
Specificity
Glutamate Receptor
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:1000
Application Notes
This metabotropic Glutamate Receptor 1a antibody is useful for Western Blot, Immunohistochemistry-Frozen and Immunohistochemistry-paraffin applications. Use in Immunocytochemistry/immunofluorescence was reported in scientific literature (PMID: 19688350) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
132.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NMDAR1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB110-39033 in the following applications:

Publications
Read Publications using
NB110-39033 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for mGluR1 Antibody - BSA Free

  • glutamate receptor, metabotropic 1
  • GPRC1A
  • GRM1
  • GRM1A
  • metabotropic glutamate receptor 1
  • mGluR1
  • MGLUR1A
  • MGLUR1GPRC1AmGlu1

Background

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GRIA1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-126
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
NB300-124
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
NLS4713
Species: Hu, Pm, Mu, Po, Pm, Rb
Applications: IHC, IHC-P, WB
NBP1-88191
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NLS3208
Species: Bv, Eq, Hu, Pm, Po, Pm
Applications: ICC, IHC, IHC-P, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-44999
Species: Hu, Mu
Applications: ICC/IF, WB
NBP3-17099
Species: Hu
Applications: IHC, IHC-P
NBP1-88323
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NLS921
Species: Bv, Hu
Applications: ICC, IHC, IHC-P, WB
H00000761-M02
Species: Hu, Mu
Applications: ELISA, Func, IHC, IHC-P, S-ELISA, WB
AF2364
Species: Hu
Applications: IHC, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NBP1-89765
Species: Hu
Applications: IHC, IHC-P
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB5277
Species: Hu
Applications: IHC, WB
NB300-189
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP2-01770
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00009455-D01P
Species: Hu, Mu
Applications: ICC/IF, WB
NB110-39033
Species: Hu, Mu, Rt, Ch
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for mGluR1 Antibody (NB110-39033)(3)

Review for mGluR1 Antibody (NB110-39033) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB110-39033:
Filter by Applications
Immunocytochemistry
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry mGluR1 NB110-39033
Enlarge
4
reviewed by:
Verified Customer
Immunocytochemistry Rat 03/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary rat cortical neurons
SpeciesRat

Comments

CommentsFixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT
Quenching: 0.1% NaBH4 in PBS, 7 minutes RT
Blocking: 3% BSA in PBS, 30 minutes RT
Permeabilization: 0.1% Triton-X100, 10 minutes RT
Primary Antibody: 1:500 in 3% BSA in PBS, overnight, 4°C
Wash: three times wash with PBS, 10 min each
Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:1800 in PBS with 1% BSA, 2 hours, RT
Wash: three times wash with PBS, 10 min each
Microscope: Bruker Vutara 350

The antibody labels clusters of metabotropic receptors along the whole cell.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mGluR1 Antibody (NB110-39033) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional mGluR1 Products

Bioinformatics Tool for mGluR1 Antibody (NB110-39033)

Discover related pathways, diseases and genes to mGluR1 Antibody (NB110-39033). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for mGluR1 Antibody (NB110-39033)

Discover more about diseases related to mGluR1 Antibody (NB110-39033).
 

Pathways for mGluR1 Antibody (NB110-39033)

View related products by pathway.

PTMs for mGluR1 Antibody (NB110-39033)

Learn more about PTMs related to mGluR1 Antibody (NB110-39033).
 

Research Areas for mGluR1 Antibody (NB110-39033)

Find related products by research area.

Blogs on mGluR1

There are no specific blogs for mGluR1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
03/27/2017
Application: Immunocytochemistry
Species: Rat

Bioinformatics

Gene Symbol GRM1
Entrez
Uniprot