mGluR6 Antibody

Images

 
Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

mGluR6 Antibody Summary

Immunogen
C-terminus of rat metabotropic Glutamate Receptor 6 [UniProt# P35349]
Localization
Integral membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRM6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Frozen
Application Notes
This metabotropic Glutamate Receptor 6 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~96 kDa. Neurons of expected brain regions stain well. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
mGluR6 Peptide (NB300-189PEP)
Publications
Read Publications using
NB300-189 in the following applications:

Reactivity Notes

Mouse and rat. Reactivity has been poor on human brain tissue.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for mGluR6 Antibody

  • CSNB1B
  • glutamate receptor, metabotropic 6
  • Gm3
  • GPRC1F
  • GPRC1Fmetabotropic glutamate receptor 6
  • GRM6
  • mGlu6
  • mGluR6
  • MGLUR6DKFZp686H1993

Background

Metabotropic Glutamate Receptor 6 (Mglur6 or Grm6), member of class III of Mglur family/GPCR3 family, is a multi-pass membrane protein localized in cell membrane with restricted expression in retinal inner nuclear layer on dendrites of ON-bipolar cells wherein it interact with effector G protein G0 alpha and act as a receptor for glutamate. An increment of light intensity in retina hyperpolarizes the photoreceptor to set off two opposing signals in OFF and ON bipolar cells, whereas in darkness, glutamate released by photoreceptors depolarizes OFF bipolar cells with hyperpolarization of ON bipolar cells. This ON bipolar cell "sign inverting" cascade involves glutamate mediated activation of Mglur6 leading to G0 alpha stimulation which results in closure of a presumably constitutively active nonselective cation channel TRPM1 (transient receptor potential melastatin 1). mGluR6 activity is mediated by a G-protein that inhibits adenylate cyclase activity and mGluR6 is the only known mGluR that mediates direct synaptic transmission in the nervous system with its role in mediating ON-response in vertebrate retinal ON-pathway. Genetic inactivation of mGluR6 in murine model blocks the ON-response and in humans, mutations in the mGluR6 gene have been linked to congenital stationary night blindness, characterized in the electroretinogram by the absence of an ON response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for mGluR6 Antibody (NB300-189)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mGluR6 Antibody (NB300-189) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-189AF405
Alexa Fluor 488 Labeled NB300-189AF488
Alexa Fluor 647 Labeled NB300-189AF647
Alexa Fluor 700 Labeled NB300-189AF700
Biotin Labeled NB300-189B
DyLight 488 Labeled NB300-189G
DyLight 550 Labeled NB300-189R
DyLight 650 Labeled NB300-189C
HRP Labeled NB300-189H

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Bioinformatics

Gene Symbol GRM6
Entrez