mGluR6 Antibody

Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.

Immunocytochemistry/Immunofluorescence: mGluR6 Antibody [NB300-189] - metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 conjugated secondary antibodies (red). DAPI was used to counterstain cell nuclei.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

mGluR6 Antibody Summary

• Immunogen: C-terminus of rat metabotropic Glutamate Receptor 6 (mGluR6) [Uniprot: P35349]
• Localization: Integral membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:1000
• Application Notes: In Western blot, a band is seen at ~96 kDa. Neurons of expected brain regions stain well
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  mGluR6 Peptide (NB300-189PEP)

Reactivity Notes

Reactivity has been poor on human brain tissue.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for mGluR6 Antibody

• CSNB1B
• glutamate receptor, metabotropic 6
• Gm3
• GPRC1F
• GPRC1Fmetabotropic glutamate receptor 6
• GRM6
• mGlu6
• mGluR6
• MGLUR6DKFZp686H1993

Background

Metabotropic Glutamate Receptor 6 (Mglur6 or Grm6), member of class III of Mglur family/GPCR3 family, is a multi-pass membrane protein localized in cell membrane with restricted expression in retinal inner nuclear layer on dendrites of ON-bipolar cells wherein it interact with effector G protein G0 alpha and act as a receptor for glutamate. An increment of light intensity in retina hyperpolarizes the photoreceptor to set off two opposing signals in OFF and ON bipolar cells, whereas in darkness, glutamate released by photoreceptors depolarizes OFF bipolar cells with hyperpolarization of ON bipolar cells. This ON bipolar cell "sign inverting" cascade involves glutamate mediated activation of Mglur6 leading to G0 alpha stimulation which results in closure of a presumably constitutively active nonselective cation channel TRPM1 (transient receptor potential melastatin 1). mGluR6 activity is mediated by a G-protein that inhibits adenylate cyclase activity and mGluR6 is the only known mGluR that mediates direct synaptic transmission in the nervous system with its role in mediating ON-response in vertebrate retinal ON-pathway. Genetic inactivation of mGluR6 in murine model blocks the ON-response and in humans, mutations in the mGluR6 gene have been linked to congenital stationary night blindness, characterized in the electroretinogram by the absence of an ON response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mGluR6 Antibody (NB300-189)(5)

Publications using NB300-189

• Fan Y, Lai J, Yuan Y et al. Taurine Protects Retinal Cells and Improves Synaptic Connections in Early Diabetic Rats Curr. Eye Res. 2020-01-01 [PMID: 31404506]
• Yang, Y, Liu, W Et al. Tmem30a deficiency leads to retinal rod bipolar cell degeneration. J Neurochem 2019-02-01 [PMID: 30548540] (WB, Human)
• Toya S, Takatsuru Y, Kokubo M et al. Early-life-stress affects the homeostasis of glutamatergic synapses. Eur J Neurosci. 2014-09-13 [PMID: 25220177]
• Gu L, Liang X, Wang L, Yan Y, Ni Z, Dai H, Gao J, Mou S, Wang Q, Chen X, Wang L, Qian J. Functional metabotropic glutamate receptors 1 and 5 are expressed in murine podocytes. Kidney Int;81(5):458-68. 2012-03-01 [PMID: 22166849] (WB, IHC-Fr, Mouse)
• Takada Y, Vijayasarathy C, Zeng Y, Kjellstrom S, Bush RA, Sieving PA. Synaptic pathology in retinoschisis knockout (Rs1-/y) mouse retina and modification by rAAV-Rs1 gene delivery. Invest Ophthalmol Vis Sci;49(8):3677-86. 2008-08-01 [PMID: 18660429] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: GRM6