mGluR8 Antibody (476410)

mGluR8 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human mGluR8 Monoclonal Antibody (Catalog # MAB5277) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 476410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

mGluR8 Antibody (476410) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human mGluR8
  Gln34-Ser514 (predicted)
  Accession # O00222
• Specificity: Detects human mGluR8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human mGluR1, 2, 3, 4, 5, or 7 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRM8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for mGluR8 Antibody (476410)

• FLJ41058
• glutamate metabotropic receptor 8
• glutamate receptor, metabotropic 8
• GPRC1H
• GPRC1HMGLUR8GLUR8
• GRM8
• metabotropic glutamate receptor 8
• MGC126724
• mGlu8
• mGluR8

Background

mGluR8 is a Group III metabotropic glutamate receptor. These 7-transmembrane glycoproteins are negatively coupled to adenylate cyclase and expressed presynaptically throughout the brain. mGluR8 shows pronounced expression in the dentate gyrus and CA3 regions of the hippocampus, and is thought to play a role in memory formation. Two splice forms, A and B, differ only in the C-terminal 16 amino acids (aa 893-909), while splice form C diverges at aa 454 and is truncated prior to the first transmembrane segment. Within the N-terminal extracellular region used as an immunogen (aa 34-514), human, mouse and rat mGluR8 share 98%-99% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRM8
• Uniprot:
  • O00222()