MFG-E8 Antibody [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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MFG-E8 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MFG-E8 (R&D Systems, Catalog # 2805-MF)
Ala23-Cys463
Accession # P21956
Specificity
Detects mouse MFG-E8 in direct ELISAs and Western blots. In these formats, less than 2% cross‑reactivity with recombinant human MFG-E8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MFGE8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 50 ug/mL
Publications
Read Publications using
AF2805 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFG-E8 Antibody [Unconjugated]

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MFG-E8 Antibody (AF2805)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(1)
Western Blot
(5)
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Mouse
(6)
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Showing Publications 1 - 7 of 7.
Publications using AF2805 Applications Species
J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018;24(8):1939-1948.e4. 2018 [PMID: 30134156] (Western Blot, Mouse) Western Blot Mouse
CA Reardon, A Lingaraju, KQ Schoenfelt, G Zhou, C Cui, H Jacobs-El, I Babenko, A Hoofnagle, D Czyz, H Shuman, T Vaisar, L Becker Obesity and Insulin Resistance Promote Atherosclerosis through an IFN?-Regulated Macrophage Protein Network Cell Rep, 2018;23(10):3021-3030. 2018 [PMID: 29874587] (Western Blot, Mouse) Western Blot Mouse
Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release JCI Insight, 2018;3(8):. 2018 [PMID: 29669945] (Western Blot, Mouse) Western Blot Mouse
JH Wang, AV Forterre, J Zhao, DO Frimannsso, A Delcayre, TJ Antes, B Efron, SS Jeffrey, MD Pegram, AC Matin Anti-HER2 scFv-directed extracellular vesicle-mediated mRNA-based gene delivery inhibits growth of HER2-positive human breast tumor xenografts by prodrug activation Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29483213] (Western Blot, Mouse) Western Blot Mouse
SA Yuzwa, MJ Borrett, BT Innes, A Voronova, T Ketela, DR Kaplan, GD Bader, FD Miller Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling Cell Rep, 2017;21(13):3970-3986. 2017 [PMID: 29281841] (IHC, Mouse) IHC Mouse
Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice Sci Rep, 2016;6(0):31687. 2016 [PMID: 27538453]
Fuller AD, Van Eldik LJ, MFG-E8 regulates microglial phagocytosis of apoptotic neurons. J Neuroimmune Pharmacol, 2008;3(4):246-56. 2008 [PMID: 18670887] (Western Blot, Mouse) Western Blot Mouse

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF2805

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Bioinformatics

Gene Symbol MFGE8
Uniprot