MFG-E8 Antibodies: Novus Biologicals

AF2805

MFG-E8 Antibody [Unconjugated]

100 μg

AF2805

HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of recombinant mouse (rm) MFG-E8 , loaded at 50 ng/mL. A specific band was detected for MFG-E8 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, re

<b>Biological Strategies Validation. </b>MFG-E8 deficiency impairs wound closure and wound angiogenesis.a The serum levels of MF-E8 in HCs (n = 30), diabetes (n = 33), and DFU patients (n = 25) were detected with ELISA. b The serum levels of MFG-E8 in normal or diabetic mice (n = 6) post-wounding for 0, 3, 7, 14 days were determined by ELISA. c Western blot analysis of MFG-E8 expression in skin tissues of normal or diabetic mice after wound for 3 days, GAPDH as a loading control. d The serum fed glucose levels of WT (n = 24) or Mfge8−/− mice (n = 28) when treated with vehicle or STZ for 28 days were measured. e Representative digital imaging of wounds from age-matched diabetic WT or Mfge8−/− mice postwounding up to day 14. f Wound closures kinetics of diabetic WT and Mfge8−/− mice (n = 6). g Representative images of H&E-stained skin tissues from WT or Mfge8−/− mice injected with vehicle or STZ after wound for 3 days. h The amount of CD31+ epithelial cells (ECs) and alpha -smooth muscle actin ( alpha -SMA+) pericytes in wound skins of WT or Mfge8−/− mice treated with vehicle or STZ at day 14 after wound. i Quantification of alpha -SMA+ vessels in five random microscopic fields in n = 6 mice per group was performed. For all experiments, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32963812), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>The activation of NLRP3 inflammasome was aggravated in MFG-E8-deficient mice.a The infiltrated active caspase-1+ and IL-1 beta + macrophages in dermis of wound skins from WT or Mfge8−/− mice (n = 6) treated with vehicle or STZ, post-wounding at day 3, were determined with immunofluorescence. b Quantification of the expression of active caspase-1, caspase-1, and MFG-E8 in wound skin tissue from WT or Mfge8−/− mice (n = 6) treated with vehicle or STZ at day 3 post-wounding, beta -actin as loading control. c Calculation of the ratio of active caspase-1/caspase-1 in wound skin tissues from each group mice. d Infiltration of death cells into wound skin tissues of WT or Mfge8−/− mice (n = 6) post-wounding at day 3 was identified by TUNEL. Representative images were shown, original magnification ×200. e The average TUNEL-positive cells were counted at more than five random microscopic fields. For all experiments, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32963812), licensed under a CC-BY license. Not internally tested by R&D Systems.

MFGE8 regulates TG hydrolase activity through CES1D(A and B) (A) Representative western blot showing CES1D protein level in WT and Mfge8 KO primary enterocytes from three independent experiments. GAPDH was used as loading control. N = 8 mice per group. (B) Densitometric analysis of the western blots (including A).(C) Representative western blot showing CES1D protein level in WT and beta 5 KO primary enterocytes from two independent experiments. HSP90 was used as loading control. N = 5 WT and N = 4 beta 5 KO mice in total.(D) Densitometric analysis of the western blots of CES1D protein (including C).(E) TG hydrolase activity in WT, Ces1d KO, and Mfge8 KO primary enterocytes 1 h after incubation with rMFGE8 or RGE. N = 5 independent experiments.(F) Western blot of CES1D and MFGE8 protein levels in proximal small intestinal enterocytes of Mfge8 KO mice with transgenic inducible expression of MFGE8 in enterocytes (MFGE8 re-expressed, Vil rtTA+ TetO Mfge8+) and single transgenic (control), WT, and Mfge8 KO enterocyte controls. GAPDH was used as loading control.(G) Densitometric analysis of the blot presented in (F).(H) TG hydrolase activity in primary enterocytes isolated from the same groups of mice in (E) and (F). N = 3 mice in each group.(I and J) 3H signal in (I) the proximal jejunum and (J) serum 2 h after oral administration of [3H]oleic acid to WT, Mfge8 KO, Ces1d KO, and Mfge8/Ces1d double-KO mice. N = 3–4 mice in each group. All data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Data in (B), (D), and (G) were analyzed by unpaired t test. Data in (E) and (H) to (J) were analyzed by one-way ANOVA followed by Bonferroni’s post test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36924494), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of the surface functionalized mExo with FA. (A) Schematic illustration showing a process of surface engineering and Dox loading. The box with a blue dashed line demonstrates the modified chemical structure of 16:0 PE-FA obtained from Avanti Polar Lipids. (B) Size distribution of mExo species (mExo, mExo-FA, Dox@mExo, and Dox@mExo-FA). (C) TEM images of mExo, mExo-FA, Dox@mExo, and Dox@mExo-FA. Scale bar: 100 nm. (D) Western blotting analysis of TSG101, CD9, MFG-E8, calnexin, and casein. TSG101 and CD9, representative positive exosome markers; MFG-E8, milk-exosomal proteins; calnexin, negative marker of exosome; casein, representative contaminants from milk Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38031117), licensed under a CC-BY license. Not internally tested by R&D Systems.

2805-MF) was serially diluted and captured by Rat Anti-Mouse MFG‑E8 Monoclonal Antibody (Catalog # MAB2805) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse MFG‑E8 (Catalog # <a class=

2805-MF) was serially diluted and captured by Rat Anti-Mouse MFG‑E8 Monoclonal Antibody (Catalog # MAB2805) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2805U-100UG
Alexa Fluor 405	AF2805V-100UG
Alexa Fluor 488	AF2805G-100UG
Alexa Fluor 532	AF2805X-100UG
Alexa Fluor 594	AF2805T-100UG
Alexa Fluor 647	AF2805R-100UG
Alexa Fluor 700	AF2805N-100UG
Alexa Fluor 750	AF2805S-100UG
Alexa Fluor Plus 405	AF2805AFP405-100UG
Alexa Fluor Plus 488	AF2805AFP488-100UG
Alexa Fluor Plus 555	AF2805AFP555-100UG
Alexa Fluor Plus 594	AF2805AFP594-100UG
Alexa Fluor Plus 647	AF2805AFP647-100UG
Alexa Fluor Plus 680	AF2805AFP680-100UG
Allophycocyanin	AF2805A
Biotin	BAF2805
CoraFluor 1	AF2805CL1
DyLight 350	AF2805D
DyLight 405	AF2805E
DyLight 488	AF2805K
DyLight 550	AF2805L
DyLight 594	AF2805M
DyLight 650	AF2805W
DyLight 680	AF2805Y
DyLight 755	AF2805Z
FITC	AF2805F
HRP	AF2805H
Janelia Fluor 525	AF2805JF525
Janelia Fluor 549	AF2805I
Janelia Fluor 646	AF2805J
Janelia Fluor 669	AF2805JF669
PE	AF2805P
PerCP	AF2805C
mFluor Violet 450 SE	AF2805MFV450
mFluor Violet 500 SE	AF2805MFV500
mFluor Violet 610 SE	AF2805MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2805-SP

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AF2767

MFG-E8 Antibody [Unconjugated]

(1 Review)

(7 Publications)

100 μg

AF2767

HAF018). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows human milk. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2767) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows human milk. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2767) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (PC‑3) (NBP2-49856) and human milk, loaded at 0.5 mg/ml. A specific band was detected for MFG‑E8 at approximately 56 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2767). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2767U-100UG
Alexa Fluor 405	AF2767V-100UG
Alexa Fluor 488	AF2767G-100UG
Alexa Fluor 532	AF2767X-100UG
Alexa Fluor 594	AF2767T-100UG
Alexa Fluor 647	AF2767R-100UG
Alexa Fluor 700	AF2767N-100UG
Alexa Fluor 750	AF2767S-100UG
Alexa Fluor Plus 405	AF2767AFP405-100UG
Alexa Fluor Plus 488	AF2767AFP488-100UG
Alexa Fluor Plus 555	AF2767AFP555-100UG
Alexa Fluor Plus 594	AF2767AFP594-100UG
Alexa Fluor Plus 647	AF2767AFP647-100UG
Alexa Fluor Plus 680	AF2767AFP680-100UG
Allophycocyanin	AF2767A
Biotin	AF2767B
CoraFluor 1	AF2767CL1
DyLight 350	AF2767D
DyLight 405	AF2767E
DyLight 488	AF2767K
DyLight 550	AF2767L
DyLight 594	AF2767M
DyLight 650	AF2767W
DyLight 680	AF2767Y
DyLight 755	AF2767Z
FITC	AF2767F
HRP	AF2767H
Janelia Fluor 525	AF2767JF525
Janelia Fluor 549	AF2767I
Janelia Fluor 646	AF2767J
Janelia Fluor 669	AF2767JF669
PE	AF2767P
PerCP	AF2767C
mFluor Violet 450 SE	AF2767MFV450
mFluor Violet 500 SE	AF2767MFV500
mFluor Violet 610 SE	AF2767MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2767-SP

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MAB27671

MFG-E8 Antibody (278918) [Unconjugated]

(5 Publications)

100 μg

MAB27671

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." alt="Human immature dendritic cells were stained with Mouse Anti-Human MFG-E8 Monoclonal Antibody (Catalog # MAB27671, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with FC012 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules." class="big_lightbox">

TF-1 cells were stained with Mouse Anti-Human MFG‑E8 Monoclonal Antibody (Catalog # MAB27671, filled histogram) or isotype control antibody (Catalog # <a class=

MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with FC012 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC27671U-100UG
Alexa Fluor 405	IC27671V-100UG
Alexa Fluor 488	IC27671G-100UG
Alexa Fluor 532	FAB27671X
Alexa Fluor 594	IC27671T-100UG
Alexa Fluor 647	IC27671R-100UG
Alexa Fluor 700	IC27671N-100UG
Alexa Fluor 750	IC27671S-100UG
Alexa Fluor Plus 405	FAB27671AFP405-100UG
Alexa Fluor Plus 488	FAB27671AFP488-100UG
Alexa Fluor Plus 555	FAB27671AFP555-100UG
Alexa Fluor Plus 594	FAB27671AFP594-100UG
Alexa Fluor Plus 647	FAB27671AFP647-100UG
Alexa Fluor Plus 680	FAB27671AFP680-100UG
Biotin	FAB27671B
CoraFluor 1	FAB27671CL1
DyLight 350	FAB27671D
DyLight 405	FAB27671E
DyLight 488	FAB27671K
DyLight 550	FAB27671L
DyLight 594	FAB27671M
DyLight 650	FAB27671W
DyLight 680	FAB27671Y
DyLight 755	FAB27671Z
FITC	FAB27671F
HRP	FAB27671H
Janelia Fluor 525	FAB27671JF525
Janelia Fluor 549	FAB27671I
Janelia Fluor 646	FAB27671J
Janelia Fluor 669	FAB27671JF669
PerCP	FAB27671C
Phycoerythrin	IC27671P
mFluor Violet 450 SE	FAB27671MFV450
mFluor Violet 500 SE	FAB27671MFV500
mFluor Violet 610 SE	FAB27671MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC27671P
Carrier Free	MAB27671-SP
Carrier Free	IC27671T-100UG
Carrier Free	IC27671S-100UG
Carrier Free	IC27671R-100UG
Carrier Free	IC27671G-100UG
Carrier Free	IC27671V-100UG
Carrier Free	IC27671U-100UG
Carrier Free	IC27671N-100UG

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MAB2767

MFG-E8 Antibody (278901) [Unconjugated]

(6 Publications)

100 μg

MAB2767

HAF016). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF016). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human milk. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MFG-E8 Monoclonal Antibody (Catalog # MAB2767) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows recombinant human (rh) MFG-E8, loaded at 50 ng/mL, and human milk, loaded at 0.2 mg/mL. A specific band was detected for MFG‑E8 at approximately 57-58 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human MFG‑E8 Monoclonal Antibody (Catalog # MAB2767) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2767U-100UG
Alexa Fluor 405	FAB2767V-100UG
Alexa Fluor 488	FAB2767G-100UG
Alexa Fluor 532	FAB2767X-100UG
Alexa Fluor 594	FAB2767T-100UG
Alexa Fluor 647	FAB2767R-100UG
Alexa Fluor 700	FAB2767N-100UG
Alexa Fluor 750	FAB2767S-100UG
Alexa Fluor Plus 405	FAB2767AFP405-100UG
Alexa Fluor Plus 488	FAB2767AFP488-100UG
Alexa Fluor Plus 555	FAB2767AFP555-100UG
Alexa Fluor Plus 594	FAB2767AFP594-100UG
Alexa Fluor Plus 647	FAB2767AFP647-100UG
Alexa Fluor Plus 680	FAB2767AFP680-100UG
Allophycocyanin	FAB2767A
Biotin	FAB2767B
CoraFluor 1	FAB2767CL1
DyLight 350	FAB2767D
DyLight 405	FAB2767E
DyLight 488	FAB2767K
DyLight 550	FAB2767L
DyLight 594	FAB2767M
DyLight 650	FAB2767W
DyLight 680	FAB2767Y
DyLight 755	FAB2767Z
FITC	FAB2767F
HRP	FAB2767H
Janelia Fluor 525	FAB2767JF525
Janelia Fluor 549	FAB2767I
Janelia Fluor 646	FAB2767J
Janelia Fluor 669	FAB2767JF669
PE	FAB2767P
PerCP	FAB2767C
mFluor Violet 450 SE	FAB2767MFV450
mFluor Violet 500 SE	FAB2767MFV500
mFluor Violet 610 SE	FAB2767MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2767-SP

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MAB2805

MFG-E8 Antibody (340614)

(4 Publications)

100 μg

MAB2805

2805-MF) was serially diluted and captured by Rat Anti-Mouse MFG‑E8 Monoclonal Antibody (Catalog # MAB2805) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2805-MF) was serially diluted and captured by Rat Anti-Mouse MFG‑E8 Monoclonal Antibody (Catalog # MAB2805) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse MFG‑E8 (Catalog # 2805-MF) was serially diluted and captured by Rat Anti-Mouse MFG‑E8 Monoclonal Antibody (Catalog # MAB2805) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2805U-100UG
Alexa Fluor 405	FAB2805V-100UG
Alexa Fluor 488	FAB2805G-100UG
Alexa Fluor 532	FAB2805X-100UG
Alexa Fluor 594	FAB2805T-100UG
Alexa Fluor 647	FAB2805R-100UG
Alexa Fluor 700	FAB2805N-100UG
Alexa Fluor 750	FAB2805S-100UG
Alexa Fluor Plus 405	FAB2805AFP405-100UG
Alexa Fluor Plus 488	FAB2805AFP488-100UG
Alexa Fluor Plus 555	FAB2805AFP555-100UG
Alexa Fluor Plus 594	FAB2805AFP594-100UG
Alexa Fluor Plus 647	FAB2805AFP647-100UG
Alexa Fluor Plus 680	FAB2805AFP680-100UG
Allophycocyanin	FAB2805A
Biotin	FAB2805B
CoraFluor 1	FAB2805CL1
DyLight 350	FAB2805D
DyLight 405	FAB2805E
DyLight 488	FAB2805K
DyLight 550	FAB2805L
DyLight 594	FAB2805M
DyLight 650	FAB2805W
DyLight 680	FAB2805Y
DyLight 755	FAB2805Z
FITC	FAB2805F
HRP	FAB2805H
Janelia Fluor 525	FAB2805JF525
Janelia Fluor 549	FAB2805I
Janelia Fluor 646	FAB2805J
Janelia Fluor 669	FAB2805JF669
PE	FAB2805P
PerCP	FAB2805C
mFluor Violet 450 SE	FAB2805MFV450
mFluor Violet 500 SE	FAB2805MFV500
mFluor Violet 610 SE	FAB2805MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2805-SP
Carrier Free	MAB2805-500

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NBP2-44412

MFG-E8 Antibody (EDM45)

0.1 mg

NBP2-44412

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (EDM45)

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (EDM45)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47813AF350
Alexa Fluor 405	NBP2-47813AF405
Alexa Fluor 488	NBP2-47813AF488
Alexa Fluor 532	NBP2-47813AF532
Alexa Fluor 594	NBP2-47813AF594
Alexa Fluor 647	NBP2-47813AF647
Alexa Fluor 700	NBP2-47813AF700
Alexa Fluor 750	NBP2-47813AF750
Alexa Fluor Plus 405	NBP2-47813AFP405
Alexa Fluor Plus 488	NBP2-47813AFP488
Alexa Fluor Plus 555	NBP2-47813AFP555
Alexa Fluor Plus 594	NBP2-47813AFP594
Alexa Fluor Plus 647	NBP2-47813AFP647
Alexa Fluor Plus 680	NBP2-47813AFP680
Allophycocyanin	NBP2-47813APC
Biotin	NBP2-47813B
CoraFluor 1	NBP2-47813CL1
DyLight 350	NBP2-47813UV
DyLight 405	NBP2-47813V
DyLight 488	NBP2-47813G
DyLight 550	NBP2-47813R
DyLight 594	NBP2-47813DL594
DyLight 650	NBP2-47813C
DyLight 680	NBP2-47813FR
DyLight 755	NBP2-47813IR
FITC	NBP2-47813F
HRP	NBP2-47813H
Janelia Fluor 525	NBP2-47813JF525
Janelia Fluor 549	NBP2-47813JF549
Janelia Fluor 646	NBP2-47813JF646
Janelia Fluor 669	NBP2-47813JF669
PE	NBP2-47813PE
PerCP	NBP2-47813PCP
mFluor Violet 450 SE	NBP2-47813MFV450
mFluor Violet 500 SE	NBP2-47813MFV500
mFluor Violet 610 SE	NBP2-47813MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47813-0.1mg
IHC-Prediluted	NBP2-48261

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NBP2-44411

MFG-E8 Antibody (SPM291)

0.1 mg

NBP2-44411

Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (SPM291).

Immunohistochemistry-Paraffin: MFG-E8 Antibody (SPM291) [NBP2-44411] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (SPM291)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11482AF350
Alexa Fluor 405	NBP3-11482AF405
Alexa Fluor 488	NBP3-11482AF488
Alexa Fluor 532	NBP3-11482AF532
Alexa Fluor 594	NBP3-11482AF594
Alexa Fluor 647	NBP3-11482AF647
Alexa Fluor 700	NBP3-11482AF700
Alexa Fluor 750	NBP3-11482AF750
Alexa Fluor Plus 405	NBP3-11482AFP405
Alexa Fluor Plus 488	NBP3-11482AFP488
Alexa Fluor Plus 555	NBP3-11482AFP555
Alexa Fluor Plus 594	NBP3-11482AFP594
Alexa Fluor Plus 647	NBP3-11482AFP647
Alexa Fluor Plus 680	NBP3-11482AFP680
Allophycocyanin	NBP3-11482APC
Biotin	NBP3-11482B
CoraFluor 1	NBP3-11482CL1
DyLight 350	NBP3-11482UV
DyLight 405	NBP3-11482V
DyLight 488	NBP3-11482G
DyLight 550	NBP3-11482R
DyLight 594	NBP3-11482DL594
DyLight 650	NBP3-11482C
DyLight 680	NBP3-11482FR
DyLight 755	NBP3-11482IR
FITC	NBP3-11482F
HRP	NBP3-11482H
Janelia Fluor 525	NBP3-11482JF525
Janelia Fluor 549	NBP3-11482JF549
Janelia Fluor 646	NBP3-11482JF646
Janelia Fluor 669	NBP3-11482JF669
PE	NBP3-11482PE
PerCP	NBP3-11482PCP
mFluor Violet 450 SE	NBP3-11482MFV450
mFluor Violet 500 SE	NBP3-11482MFV500
mFluor Violet 610 SE	NBP3-11482MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-48260
Azide and BSA Free	NBP3-11482

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NBP2-44410

MFG-E8 Antibody (MFG-06)

0.1 mg

NBP2-44410

Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (MFG-06)

Immunohistochemistry-Paraffin: MFG-E8 Antibody (MFG-06) [NBP2-44410] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (MFG-06)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11473AF350
Alexa Fluor 405	NBP3-11473AF405
Alexa Fluor 488	NBP3-11473AF488
Alexa Fluor 532	NBP3-11473AF532
Alexa Fluor 594	NBP3-11473AF594
Alexa Fluor 647	NBP3-11473AF647
Alexa Fluor 700	NBP3-11473AF700
Alexa Fluor 750	NBP3-11473AF750
Alexa Fluor Plus 405	NBP3-11473AFP405
Alexa Fluor Plus 488	NBP3-11473AFP488
Alexa Fluor Plus 555	NBP3-11473AFP555
Alexa Fluor Plus 594	NBP3-11473AFP594
Alexa Fluor Plus 647	NBP3-11473AFP647
Alexa Fluor Plus 680	NBP3-11473AFP680
Allophycocyanin	NBP3-11473APC
Biotin	NBP3-11473B
CoraFluor 1	NBP3-11473CL1
DyLight 350	NBP3-11473UV
DyLight 405	NBP3-11473V
DyLight 488	NBP3-11473G
DyLight 550	NBP3-11473R
DyLight 594	NBP3-11473DL594
DyLight 650	NBP3-11473C
DyLight 680	NBP3-11473FR
DyLight 755	NBP3-11473IR
FITC	NBP3-11473F
HRP	NBP3-11473H
Janelia Fluor 525	NBP3-11473JF525
Janelia Fluor 549	NBP3-11473JF549
Janelia Fluor 646	NBP3-11473JF646
Janelia Fluor 669	NBP3-11473JF669
PE	NBP3-11473PE
PerCP	NBP3-11473PCP
mFluor Violet 450 SE	NBP3-11473MFV450
mFluor Violet 500 SE	NBP3-11473MFV500
mFluor Violet 610 SE	NBP3-11473MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-48262
Azide and BSA Free	NBP3-11473

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NBP3-41452

MFG-E8 Antibody (C4) - BSA Free

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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FAB2767AFP488

MFG-E8 Antibody (278901) [Alexa Fluor™ Plus 488]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 488

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FAB2805AFP594

MFG-E8 Antibody (340614) [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor Plus 594

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AF2767AFP680

MFG-E8 Antibody [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Conjugate:

Alexa Fluor Plus 680

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

MFG-E8 Antibodies

MFG-E8 Antibody [Unconjugated]

MFG-E8 Antibody [Unconjugated]

MFG-E8 Antibody (278918) [Unconjugated]

MFG-E8 Antibody (278901) [Unconjugated]

MFG-E8 Antibody (340614)

MFG-E8 Antibody (EDM45)

MFG-E8 Antibody (SPM291)

MFG-E8 Antibody (MFG-06)

MFG-E8 Antibody (C4) - BSA Free

MFG-E8 Antibody (278901) [Alexa Fluor™ Plus 488]

MFG-E8 Antibody (340614) [Alexa Fluor™ Plus 594]

MFG-E8 Antibody [Alexa Fluor™ Plus 680]