MFG-E8 Antibody [Unconjugated]

Images

 
Western blot shows human milk. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2767) followed by HRP-conjugated Anti-Sheep IgG Secondary ...read more
Simple Western lane view shows recombinant human (rh) MFG-E8 and human milk, loaded at 0.2 mg/mL. A specific band was detected for MFG‑E8 at approximately 56-58 kDa (as indicated) using 50 µg/mL of Sheep ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

MFG-E8 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived human MFG-E8
Leu24-Cys387
Accession # Q08431
Specificity
Detects human MFG-E8 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MFGE8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 50 ug/mL
Control
MFG-E8 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
AF2767 in the following applications:

Publications
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AF2767 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFG-E8 Antibody [Unconjugated]

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
  3. Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
  4. Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
  5. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  6. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  7. Hanayama, R. et al. (2002) Nature 417:182.
  8. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  9. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  10. Hanayama, R. et al. (2004) Science 304:1147.
  11. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  12. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  13. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  14. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  15. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MFG-E8 Antibody (AF2767)(3)

Review for MFG-E8 Antibody (AF2767) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2767:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot MFG-E8 AF2767
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4
reviewed by:
Anonymous
WB Human 12/17/2015
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Summary

ApplicationWestern Blot
Sample Testedexosome lysate from HT1080 cell line (lysate in RIPA buffer)
SpeciesHuman
LotCCUA0111081

Blocking

Blocking Details5% blocking power in PBS + 0.05% Tween 20; 1h RT

Primary Anitbody

Dilution Ratio2ug/ml in blocking buffer; incubation at 4C overnight

Secondary Antibody

Secondary Descriptionrabbit anti-sheep HRP antibody at 1:1000 dil in blocking buffer, 1h RT
Secondary Manufacturer Cat#Thermo, ref# 61-8620
Secondary ConcentrationSuperSignal West Dura Extended Duration Substrate

Details

Detection Notesx-film exposure for 2min

Comments

CommentsGood detection of specific band corresponding to MFG-E8 protein. There was some non-specific staining of the higher molecular weight markers; this not interfere with the sample analysis as high molecular weight proteins in the sample.

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Recent Reviews

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5
0
4
1
3
0
2
0
1
0

Anonymous
12/17/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MFGE8
Uniprot