MFG-E8 Antibody [Unconjugated]

Western blot shows human milk. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2767) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MFG-E8 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (PC‑3) (NBP2-49856) and human milk, loaded at 0.5 mg/ml. A specific band was detected for MFG‑E8 at approximately 56 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human MFG‑E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2767). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

MFG-E8 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived human MFG-E8
  Leu24-Cys387
  Accession # Q08431
• Specificity: Detects human MFG-E8 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MFGE8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFG-E8 Antibody [Unconjugated]

• BA46
• Breast epithelial antigen BA46
• EDIL1
• hP47
• HsT19888
• lactadherin
• Lactahedrin
• Medin
• MFG1
• MFGE8
• MFG-E8
• MFGM
• milk fat globule-EGF factor 8 protein
• Milk fat globule-EGF factor 8
• O-acetyl disialoganglioside synthase
• OAcGD3S
• SED1
• SPAG10
• sperm associated antigen 10
• sperm surface protein hP47

Background

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).

1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
2. Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
3. Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
4. Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
5. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
6. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
7. Hanayama, R. et al. (2002) Nature 417:182.
8. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
9. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
10. Hanayama, R. et al. (2004) Science 304:1147.
11. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
12. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
13. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
14. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
15. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MFG-E8 Antibody (AF2767)(7)

Publications using AF2767

• Makis Tzioras, Michael J.D. Daniels, Caitlin Davies, Paul Baxter, Declan King, Sean McKay et al. Human astrocytes and microglia show augmented ingestion of synapses in Alzheimer’s disease via MFG-E8 Cell Reports Medicine 2023-09-19 [PMID: 37652017] (Western Blot, Dot Blot, Human)
• Makis Tzioras, Michael J.D. Daniels, Caitlin Davies, Paul Baxter, Declan King, Sean McKay et al. Human astrocytes and microglia show augmented ingestion of synapses in Alzheimer’s disease via MFG-E8 Cell Reports Medicine 9/19/2023 [PMID: 37652017]
• Wu Y, Wang F, Shi J et al. CircSMAD2 accelerates endometrial cancer cell proliferation and metastasis by regulating the miR-1277-5p/MFGE8 axis Journal of gynecologic oncology 1/2/2023 [PMID: 36659830]
• Emi Kawada-Horitani, Shunbun Kita, Tomonori Okita, Yuto Nakamura, Hiroyuki Nishida, Yoichi Honma et al. Human adipose-derived mesenchymal stem cells prevent type 1 diabetes induced by immune checkpoint blockade Diabetologia 7/1/2022 [PMID: 35511238]
• Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice Mol. Ther., 2020-07-10;0(0):. 2020-07-10 [PMID: 32652045] (Western Blot, Human)
• J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018-08-21;24(8):1939-1948.e4. 2018-08-21 [PMID: 30134156] (Western Blot, Human)
• Uchiyama A, Yamada K, Ogino S, Yokoyama Y, Takeuchi Y, Udey M, Ishikawa O, Motegi S MFG-E8 regulates angiogenesis in cutaneous wound healing. Am J Pathol, 2014-05-15;184(7):1981-90. 2014-05-15 [PMID: 24838098] (IHC, Human)

Review for MFG-E8 Antibody (AF2767) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/17/2015

Summary

• Application: Western Blot
• Sample Tested: exosome lysate from HT1080 cell line (lysate in RIPA buffer)
• Species: Human
• Lot: CCUA0111081

Bioinformatics

• Gene Symbol: MFGE8
• Uniprot:
  • Q08431()