Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Western Blot: Mer [p Tyr749, p Tyr753] Antibody [NB300-690] - Phospho & non-Phospho-MerTK with Phospho-MERTK Antibody (NB300-690). Phosphorylated (1) & un-phosphorylated (2, 3) MERTK was immunoprecipitated with MERTK and blotted with Phospho-MerTK antibody (NB300-690). MW of MERTK is 174 kDa. Lower broad band is of IgGs in the immunoprecipitates.

Immunocytochemistry/Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690] - PCaControl cells or PCaGAS6OE cells were starved for 24 hours and then exposed to 10% FBS of culture condition for 1 hour and phosphorylation of Mer was evaluated by (D) immunofluorescence staining. Bar = 50 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.8365), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690] - Left panel: Immunofluorescence staining of p-Mer (red)/GAS6 (green) in PCa cells in bone marrow of a PCa patient. Blue, DAPI nuclearstain. Bar = 50 um. Right panel: p-Mer (red)/GAS6 (green) positive cells (white arrows) in the magnification of the white rectangle from left panel. Bar = 20 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.8365), licensed under a CC-BY license.

GAS6 overexpression activates the phosphorylation of Mer signaling in prostate epithelial cells or cancer cells(A) GAS6 mRNA expression as quantified by by real-time PCR in PCaControl cells or PCaGAS6OE cells. (B) GAS6 protein as quantified by ELISA in PCaControl cells or PCaGAS6OE cells. Data in Figure 4A, B are representative of mean with s.d. (Student's t-test). (C, D). PCaControl cells or PCaGAS6OE cells were starved for 24 hours and then exposed to 10% FBS of culture condition for 1 hour and phosphorylation of Mer was evaluated by (C) Western blot or (D) immunofluorescence staining. Bar = 50 μm. (E, F) GAS6 and Mer mRNA expression in the PrEPGAS6+/+ cells or PrEPGAS6−/− cells by real-time PCR. (G) Mer (red)/GAS6 (green) or p-Mer (red)/GAS6 (green) in the PrEPGAS6+/+ cells or PrEPGAS6−/− cells was evaluated by immunofluorescence staining. Bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27028863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

GAS6 overexpression increases CSCs through activation of Mer signaling in PCa cells(A, B) Percent of cells expressing the CSC phenotype in coculture of PCaControl or PCaGAS6OE with osteoblasts as quantified by FACS analysis. Data in Figure 5A, 5B are representative of mean with s.d. (Student's t-test). (C) The sphere formation of PCaControl cells or PCaGAS6OE cells in suspended sphere culture conditions following the treatment with a Mer inhibitor, UNC1062. Bar = 50 μm. (D) Quantification of prostatospheres in suspended sphere culture condition. *Denotes p 0.05) between vehicle vs. a Mer inhibitor, UNC1062 treatments by Student's t-test. (G, H) % CSC from DTC population in bone marrow at 24 hours after intracardiac injection of PCaControl cells or PCaGAS6OE in SCID mice (n = 5) as quantified by FACS analysis. Data are representative of mean with s.d. (Student's t-test). (I) Left panel: Immunofluorescence staining of p-Mer (red)/GAS6 (green) in PCa cells in bone marrow of a PCa patient. Blue, DAPI nuclearstain. Bar = 50 μm. Right panel: p-Mer (red)/GAS6 (green) positive cells (white arrows) in the magnification of the white rectangle from left panel. Bar = 20 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27028863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Mer [p Tyr754, p Tyr749, p Tyr753] Antibody Summary

• Immunogen: Synthetic peptide: corresponding to amino acids 746-757 (CKKIYSGDYYRQG-amide) [p Y749, p Y753, p Y754] of Human MERTK.
• Modification: p Tyr749, p Tyr753, p Tyr754
• Specificity: MERTK [Tyr749/Tyr753/Tyr754]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MERTK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:250
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:250
  • Western Blot 1:750
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

• c-Eyk
• c-mer proto-oncogene tyrosine kinase
• C-mer
• EC 2.7.10
• EC 2.7.10.1
• MER receptor tyrosine kinase
• Mer
• MerTK
• MGC133349
• Receptor tyrosine kinase MerTK
• RP38Proto-oncogene c-Mer
• STK kinase
• tyrosine-protein kinase Mer

Background

MerTK (c-mer, Nyk, Eyk) is a tyrosine kinase that belongs to a family of transmembrane receptors (1). MerTk has been implicated in reversible cell growth arrest, survival, proliferation and cell adhesion. Growth arrest specific protein 6 (Gas6) is an activating ligand for MerTK (2-3). MerTK is required for clearance of apoptotic cells by mononuclear phagocytes in mice; with its absence resulting in progressive lupus like autoimmunity (4). Defects in MERTK are a cause of retinitis pigmentosa (RP) (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mer Antibody (NB300-690)(5)

Publications using NB300-690

• Jung Y, Decker AM, Wang J et al. Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow. Oncotarget 2016-05-03 [PMID: 27028863]
• Chang L, Chin Y, Wu P et al. Polymeric nano-formulation of spectrum selective RTK inhibitor strengthens anti-cancer effects via immune remodeling by endoplasmic reticulum stress-modulating mitochondrial metabolism Nano Today 2024-02-01 (WB, Mouse)
• Yefimova, M G, LefEvre, C Et al. Granulosa cells provide elimination of apoptotic oocytes through unconventional autophagy-assisted phagocytosis. Hum Reprod 2020-06-01 [PMID: 32531067] (WB, Mouse)
• Du W, Huang H, Sorrelle N, Brekken RA. Sitravatinib potentiates immune checkpoint blockade in refractory cancer models. JCI Insight. 2018-11-02 [PMID: 30385724] (WB, Mouse)
• A-Gonzalez Noelia, Bensinger Steven J, Hong Cynthia et al. Apoptotic cells promote their own clearance and immune tolerance through activation of the nuclear receptor LXR. Immunity 2009-01-01 [PMID: 19646905] (WB, Mouse)

FAQs for Mer Antibody (NB300-690). (Showing 1 - 1 of 1 FAQs).

1. What is the composition of buffer?
   • The antibody stabilization buffer contains Tris-Glycine buffer pH7.4, glycerol, stabilizing protein BSA, preservative Sodium azide at 0.02% final concentration; however, the exact composition of this buffer is proprietary.

Bioinformatics

• Gene Symbol: MERTK
• Entrez:
  • 10461(Human)
• Uniprot:
  • Q12866()