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AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox" target="_blank">
VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox" target="_blank">
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox" target="_blank">
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox" target="_blank">
MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Mer knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">
891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for Mer at approximately 175 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox" target="_blank">
891-MR), human Axl/Fc (R&D Systems, Catalog #154-AL), human Dtk/Fc (R&D Systems, Catalog #859-DK) or human TrkA/Fc (R&D Systems, Catalog #175-TK) were added to 100 μg lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human Mer. " class="big_lightbox" target="_blank">
MerTK (c-mer, Nyk, Eyk) is a tyrosine kinase that belongs to a family of transmembrane receptors (1). MerTk has been implicated in reversible cell growth arrest, survival, proliferation and cell adhesion. Growth arrest specific protein 6 (Gas6) is an activating ligand for MerTK (2-3). MerTK is required for clearance of apoptotic cells by mononuclear phagocytes in mice; with its absence resulting in progressive lupus like autoimmunity (4). Defects in MERTK are a cause of retinitis pigmentosa (RP) (5).
![Flow Cytometry Mer Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_AF591_Flow_Cytometry_12100.jpg)
![Flow Cytometry Mer Antibody (125518) [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_MAB8912_Flow_Cytometry_13084.jpg)
![Western Blot Mer Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_AF891_Western_Blot_20027.jpg)
![N/A Mer [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Mer_DY6488_ELISA_2164.jpg)
![ELISA Mer [Biotin]](http://images.novusbio.com/fullsize/dyc891-2_human-total-mer-duoset-ic-elisa-2-plate-elisa-169202091914.jpg)
![ELISA Mer [Biotin]](http://images.novusbio.com/images/dyc2579-2_human-phospho-mer-duoset-ic-elisa-2-plate-elisa-301220207159.png)
![Immunohistochemistry Mer Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_AF591_Immunohistochemistry_22755.jpg)
![Dual RNAscope ISH-IHC Compatible Mer Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_AF591_Dual_RNAscope_ISHIHC_23834.jpg)
![Western Blot Mer Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af591_mouse-mer-affinity-purified-polyclonal-ab-4120241049418.jpg)
![Flow Cytometry Mer Antibody (125518) [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_MAB8912_Flow_Cytometry_13085.jpg)
![Knockout Validated Mer Antibody (125518) [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_MAB8912_Knockout_Validated_22944.jpg)
![Flow Cytometry Mer Antibody (125518) [Unconjugated]](http://images.novusbio.com/fullsize/mab8912_human-mer-mab-clone-125518-2282024179562.jpg)
![Flow Cytometry Mer Antibody (125518) [Unconjugated]](http://images.novusbio.com/fullsize/mab8912_human-mer-mab-clone-125518-flow-cytometry-1212202575046.jpg)
![ELISA Mer Antibody (125518) [Unconjugated]](http://images.novusbio.com/images/mab8912_human-mer-mab-clone-125518-elisa-639201780469043240.jpg)
![Knockout Validated Mer Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Mer_AF891_Knockout_Validated_22993.jpg)
![Western Blot Mer Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af891_human-mer-affinity-purified-polyclonal-ab-western-blot-121220259305218.jpg)
![ELISA Mer Antibody [Unconjugated]](http://images.novusbio.com/images/af891_human-mer-affinity-purified-polyclonal-ab-elisa-639201854769552220.jpg)
![ELISA Mer [Biotin]](http://images.novusbio.com/images/dyc2579-2_human-phospho-mer-duoset-ic-elisa-2-plate-elisa-3012202074346.png)
![ELISA Mer [Biotin]](http://images.novusbio.com/images/dyc2579-2_human-phospho-mer-duoset-ic-elisa-2-plate-elisa-3012202074717.png)
![SEC-MALS Mer [Unconjugated]](http://images.novusbio.com/fullsize/11313-mr_recombinant-mouse-mer-his-tag-protein-cf-sec-mals-30720258819..jpg)
![Binding Activity Mer [Unconjugated]](http://images.novusbio.com/images/11313-mr_recombinant-mouse-mer-his-tag-protein-cf-binding-activity-1612202213239.jpg)
![SDS-Page Mer [Unconjugated]](http://images.novusbio.com/fullsize/11313-mr_recombinant-mouse-mer-his-tag-protein-cf-sds-page-1811202271339.jpg)
![Binding Activity Mer [Biotin]](http://images.novusbio.com/images/avi891_recombinant-human-mer-fc-chimera-avi-tag-protein-cf-binding-activity-412022115032.jpg)
![SDS-Page Mer [Biotin]](http://images.novusbio.com/fullsize/avi891_recombinant-human-mer-fc-chimera-avi-tag-protein-cf-sds-page-612202191536.jpg)
