Mer ELISA Kits

HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox"> HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." alt="NS0 cells transfected with human Mer (NS0-hMer) were treated with conditioned media from the human lung cell line, WI-38, for 15 minutes to induce tyrosine phosphorylation of Mer. Lysates were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-Mer monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with an HRP-conjugated anti-phosphotyrosine monoclonal antibody (R&D Systems, Catalog # HAM1676) to detect phospho-Mer. Bands were visualized by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (R&D Systems, Catalog # AR004). Human Phospho-Mer can be detected in this DuoSet IC ELISA by using approximately 5 to 10 times less lysate than is needed for a conventional IP-Western blot." class="big_thumb" />

BAF891). " class="big_lightbox"> BAF891). " class="big_thumb" />

891-MR), human Axl/Fc (R&D Systems, Catalog #154-AL), human Dtk/Fc (R&D Systems, Catalog #859-DK) or human TrkA/Fc (R&D Systems, Catalog #175-TK) were added to 100 μg lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human Mer. " class="big_lightbox"> 891-MR), human Axl/Fc (R&D Systems, Catalog #154-AL), human Dtk/Fc (R&D Systems, Catalog #859-DK) or human TrkA/Fc (R&D Systems, Catalog #175-TK) were added to 100 μg lysate and analyzed using this DuoSet IC ELISA. Competition was observed only with recombinant human Mer. " class="big_thumb" />