Mer Antibody (108928) [Unconjugated]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Mer Monoclonal Antibody (Catalog # MAB5912, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 108928
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Mer Antibody (108928) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
  Glu23-Phe498
  Accession # Q60805
• Specificity: Detects mouse Mer in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mer Antibody (108928) [Unconjugated]

• c-Eyk
• c-mer proto-oncogene tyrosine kinase
• C-mer
• EC 2.7.10
• EC 2.7.10.1
• MER receptor tyrosine kinase
• Mer
• MerTK
• MGC133349
• Receptor tyrosine kinase MerTK
• RP38Proto-oncogene c-Mer
• STK kinase
• tyrosine-protein kinase Mer

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mer Antibody (MAB5912)(2)

Publications using MAB5912

• ME Ogle, JR Krieger, LE Tellier, J McFaline-F, JS Temenoff, EA Botchwey Dual Affinity Heparin-Based Hydrogels Achieve Pro-Regenerative Immunomodulation and Microvascular Remodeling ACS Biomater Sci Eng, 2017-02-20;4(4):1241-1250. 2017-02-20 [PMID: 29682605] (Flow Cytometry, Mouse)
• K Fujiu, M Shibata, Y Nakayama, F Ogata, S Matsumoto, K Noshita, S Iwami, S Nakae, I Komuro, R Nagai, I Manabe A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation Nat. Med., 2017-04-10;23(5):611-622. 2017-04-10 [PMID: 28394333] (Flow Cytometry, Mouse)

Bioinformatics

• Uniprot:
  • Q60805()