Mer Antibody (108928) [Phycoerythrin]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Mer PE-conjugated Monoclonal Antibody (Catalog # FAB5912P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 108928
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Mer Antibody (108928) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
  Glu23-Phe498
  Accession # Q60805
• Specificity: Detects mouse Mer in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MERTK
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mer Antibody (108928) [Phycoerythrin]

• c-Eyk
• c-mer proto-oncogene tyrosine kinase
• C-mer
• EC 2.7.10
• EC 2.7.10.1
• MER receptor tyrosine kinase
• Mer
• MerTK
• MGC133349
• Receptor tyrosine kinase MerTK
• RP38Proto-oncogene c-Mer
• STK kinase
• tyrosine-protein kinase Mer

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mer Antibody (FAB5912P)(8)

Publications using FAB5912P

• SP Kubli, L Vornholz, G Duncan, W Zhou, P Ramachandr, J Fortin, M Cox, S Han, R Nechanitzk, D Nechanitzk, BE Snow, L Jones, WY Li, J Haight, A Wakeham, MR Bray, TW Mak Fcmr regulates mononuclear phagocyte control of anti-tumor immunity Nat Commun, 2019-06-18;10(1):2678. 2019-06-18 [PMID: 31213601] (Flow Cytometry, Mouse, Transgenic Mouse)
• SP Kubli, C Bassi, C Roux, A Wakeham, C Göbl, W Zhou, SM Jafari, B Snow, L Jones, L Palomero, KL Thu, L Cassetta, D Soong, T Berger, P Ramachandr, SP Baniasadi, G Duncan, M Lindzen, Y Yarden, C Herranz, C Lazaro, MF Chu, J Haight, P Tinto, J Silvester, DW Cescon, A Petit, S Pettersson, JW Pollard, TW Mak, MA Pujana, P Cappello, C Gorrini AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer Proc. Natl. Acad. Sci. U.S.A., 2019-02-07;0(0):. 2019-02-07 [PMID: 30733286] (Flow Cytometry, Mouse)
• CE Macdougall, EG Wood, J Loschko, V Scagliotti, FC Cassidy, ME Robinson, N Feldhahn, L Castellano, MB Voisin, F Marelli-Be, C Gaston-Mas, M Charalambo, MP Longhi Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets Cell Metab., 2018-03-06;27(3):588-601.e4. 2018-03-06 [PMID: 29514067] (Flow Cytometry, Mouse)
• Zhiqiang Guo, Yan Li, Dandan Zhang, Jiaying Ma Axl inhibition induces the antitumor immune response which can be further potentiated by PD-1 blockade in the mouse cancer models Oncotarget 10/27/2017 [PMID: 29163786]
• GHY Lin, V Chai, V Lee, K Dodge, T Truong, M Wong, LD Johnson, E Linderoth, X Pang, J Winston, PS Petrova, RA Uger, NN Viller TTI-621 (SIRP?Fc), a CD47-blocking cancer immunotherapeutic, triggers phagocytosis of lymphoma cells by multiple polarized macrophage subsets PLoS ONE, 2017-10-30;12(10):e0187262. 2017-10-30 [PMID: 29084248] (Flow Cytometry, Human)
• Cnops J, De Trez C, Stijlemans B, Keirsse J, Kauffmann F, Barkhuizen M, Keeton R, Boon L, Brombacher F, Magez S NK-, NKT- and CD8-Derived IFNgamma Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia. PLoS Pathog, 2015-06-12;11(6):e1004964. 2015-06-12 [PMID: 26070118] (Flow Cytometry, Mouse)
• Stijlemans , Benoit, Cnops , Jennifer, Naniima , Peter, Vaast , Axel, Bockstal , Viki, De Baetselier , Patrick, Magez , Stefan Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis. PLoS Negl Trop Dis, 2015-03-05;9(3):e0003561. 2015-03-05 [PMID: 25742307] (Flow Cytometry, Mouse)
• Taichman , Russell, Patel , Lalit R, Bedenis , Rachel, Wang , Jingchen, Weidner , Savannah, Schumann , Taibrian, Yumoto , Kenji, Berry , Janice E, Shiozawa , Yusuke, Pienta , Kenneth GAS6 receptor status is associated with dormancy and bone metastatic tumor formation. PLoS ONE, 2013-04-24;8(4):e61873. 2013-04-24 [PMID: 23637920] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: MERTK
• Uniprot:
  • Q60805()