Mer Antibody (108928) [Allophycocyanin]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Mer APC-conjugated Monoclonal Antibody (Catalog # FAB5912A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 108928
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Mer Antibody (108928) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
  Glu23-Phe498
  Accession # Q60805
• Specificity: Detects mouse Mer in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MERTK
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mer Antibody (108928) [Allophycocyanin]

• c-Eyk
• c-mer proto-oncogene tyrosine kinase
• C-mer
• EC 2.7.10
• EC 2.7.10.1
• MER receptor tyrosine kinase
• Mer
• MerTK
• MGC133349
• Receptor tyrosine kinase MerTK
• RP38Proto-oncogene c-Mer
• STK kinase
• tyrosine-protein kinase Mer

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein Growth-Arrest-Specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mer Antibody (FAB5912A)(7)

Publications using FAB5912A

• Tariq Hamid, Yuanyuan Xu, Mohamed Ameen Ismahil, Gregg Rokosh, Miki Jinno, Guihua Zhou et al. Cardiac Mesenchymal Stem Cells Promote Fibrosis and Remodeling in Heart Failure: Role of PDGF Signaling JACC: Basic to Translational Science 2022-05-01 [PMID: 35663630]
• DM Patrick, N de la Visi, J Krishnan, W Chen, MJ Ormseth, CM Stein, SS Davies, V Amarnath, LJ Crofford, JM Williams, S Zhao, CD Smart, S Dikalov, A Dikalova, L Xiao, JP Van Beusec, M Ao, AB Fogo, A Kirabo, DG Harrison Isolevuglandins disrupt PU.1-mediated C1q expression and promote autoimmunity and hypertension in systemic lupus erythematosus JCI Insight, 2022-07-08;7(13):. 2022-07-08 [PMID: 35608913] (Flow Cytometry, Mouse)
• Senthil Kumaran Satyanarayanan, Driss El Kebir, Soaad Soboh, Sergei Butenko, Meriem Sekheri, Janan Saadi et al. IFN-beta is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation Nature Communications 2019-08-02 [PMID: 31375662]
• C Miyabe, Y Miyabe, L Moreno, J Lian, RA Rahimi, NN Miura, N Ohno, Y Iwakura, T Kawakami, AD Luster Dectin-2-induced CCL2 production in tissue-resident macrophages ignites cardiac arteritis J. Clin. Invest., 2019-06-06;130(0):. 2019-06-06 [PMID: 31169521] (Flow Cytometry, Mouse)
• JA Santamaria, S Zeng, JB Greer, MJ Beckman, AM Seifert, NA Cohen, JQ Zhang, MH Crawley, BL Green, JK Loo, JH Maltbaek, RP DeMatteo Csf1r or Mer inhibition delays liver regeneration via suppression of Kupffer cells PLoS ONE, 2019-05-01;14(5):e0216275. 2019-05-01 [PMID: 31042769] (Flow Cytometry, Mouse)
• MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation Proc Natl Acad Sci USA, 2016-05-19;0(0):. 2016-05-19 [PMID: 27199481] (Flow Cytometry, Mouse)
• Crane , Meredith, Daley , Jean M, van Houtte , Olivier, Brancato , Samielle, Henry , William, Albina , Jorge E The monocyte to macrophage transition in the murine sterile wound. PLoS ONE, 2014-01-22;9(1):e86660. 2014-01-22 [PMID: 24466192] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: MERTK
• Uniprot:
  • Q60805()