Melan-A/MART-1 Antibody (M2-7C10) [Biotin]

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Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
M2-7C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Melan-A/MART-1 Antibody (M2-7C10) [Biotin] Summary

Immunogen
Recombinant human Melan-A protein was used as immunogen to generate the Melan-A antibody.
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Melan-A/MART-1 Antibody (M2-7C10) [Biotin]

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP2-33148B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33148AF350
Alexa Fluor 405 NBP2-33148AF405
Alexa Fluor 488 NBP2-33148AF488
Alexa Fluor 532 NBP2-33148AF532
Alexa Fluor 594 NBP2-33148AF594
Alexa Fluor 647 NBP2-33148AF647
Alexa Fluor 700 NBP2-33148AF700
Alexa Fluor 750 NBP2-33148AF750
Biotin NBP2-33148B
DyLight 350 NBP2-33148UV
DyLight 405 NBP2-33148V
DyLight 488 NBP2-33148G
DyLight 550 NBP2-33148R
DyLight 650 NBP2-33148C
DyLight 680 NBP2-33148FR
DyLight 755 NBP2-33148IR
HRP NBP2-33148H
Janelia Fluor 549 NBP2-33148JF549
Janelia Fluor 646 NBP2-33148JF646
PerCP NBP2-33148PCP

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Bioinformatics Tool for Melan-A/MART-1 Antibody (NBP2-33148B)

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Bioinformatics

Gene Symbol MLANA
Entrez