Melan-A/MART-1 Antibody (M2-7C10)

Images

 
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).
Flow Cytometry: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Flow Cytometry: Melan-A/MART-1 Antibody (M2-7C10) [Alexa Fluor® 647] [NBP2-33148AF647] - An intracellular stain was performed on SK-MEL-28 cells with ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.
Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using ...read more
SDS-Page: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197]

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PAGE
Clone
M2-7C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Melan-A/MART-1 Antibody (M2-7C10) Summary

Immunogen
Recombinant human Melan-A protein was used as immunogen to generate the Melan-A antibody.
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
  • SDS-Page
Application Notes
Mol. Weight of Antigen: 20-22kDa (doublet)
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation and Western Blot applications cited in literature. Use in Immunohistochemistry reported in scientific literature (PMID: 31024232). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Publications
Read Publications using
NBP2-15197 in the following applications:

  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for Melan-A/MART-1 Antibody (M2-7C10)

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognize by T cells 1), also known as Melan-A, is an 18 kDa melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. MART-1 is expressed in melanosomes and the endoplasmic reticulum. MART-1 is the most widely used marker for identifying malignant melanoma, the most deadly form of skin cancer, and facilitating complete removal of the primary tumor (Campoli, 2012). In this regard, MART-1 is used both as a confirmatory marker for melanocyte differentiation in S100 (protein present in melanocytes) positive lesions and a primary marker to evaluate the extent of melanocyte tumors (Ohsie, 2012, Collins, 2012). MART-1 specific monoclonal antibodies have high sensitivity (75-92%) and specificity (95-100%) for melanoma (Campoli, 2012, Oshie et.al, 2012).
The clone M2-7C10 MART-1 antibody labels melanomas and other tumors showing melanocyte differentiation, and is widely used for assessing melanomas (Campoli, 2012, Ohsie, 2012, Collins, 2012). Analysis of melanoma lesions with this antibody shows that there is significant heterogeneity of expression of MART-1 both as a percentage of cells and in intensity of expression (Marincola, 1996). The reactivity of the MART-1 antibody is not restricted to melanoma, and the antibody has also been shown to label some mesenchymal tumors and sarcomas (Campoli, 2012).
The exact eptiope recognized by this MART-1 antibody has not been mapped. However, the MART-1 epitope recognized by this antibody appears to be different from that recognized by the M2-9E3 MART-1 antibody clone (ABX-126A) (Kawakami, 1997). Researchers often use more than one antibody against a given specificity to help follow up and validate results. Hence, it may be useful to use both the ABX-125A and ABX-126A antibodies in parallel to obtain additional information about MART-1 expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melan-A/MART-1 Antibody (NBP2-15197)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(2)
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Human
(1)
Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-15197 Applications Species
Yang S, Wei J, Cui YH et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade Nat Commun. Jun 25 2019 [PMID: 31239444] (IHC, Human) IHC Human
Prakash, R;Izraely, S;Thareja, NS;Lee, RH;Rappaport, M;Kawaguchi, R;Sagi-Assif, O;Ben-Menachem, S;Meshel, T;Machnicki, M;Ohe, S;Hoon, DS;Coppola, G;Witz, IP;Carmichael, ST; Regeneration Enhances Metastasis: A Novel Role for Neurovascular Signaling in Promoting Melanoma Brain Metastasis Front Neurosci Apr 9 2019 [PMID: 31024232] (IHC, Mouse) IHC Mouse
Collins GR, Essary L, Strauss J et al. Incidentally discovered distant cutaneous metastasis of sacral chordoma: a case with variation in S100 protein expression (compared to the primary tumor) and review of the literature. J Cutan Pathol. 2012 Jun [PMID: 22524546]
Mihic-Probst D, Ikenberg K, Tinguely M et al. Tumor cell plasticity and angiogenesis in human melanomas. PLoS One. 2012 [PMID: 22442699]
Hoashi T, Watabe H, Muller J et al. MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes. J Biol Chem. 2005 Apr 8 [PMID: 15695812]
Kawakami Y, Battles JK, Kobayashi T et al. Production of recombinant MART-1 proteins and specific antiMART-1 polyclonal and monoclonal antibodies: use in the characterization of the human melanoma antigen MART-1. J Immunol Methods. 1997 Mar 10 [PMID: 9075767]
Marincola FM, Hijazi YM, Fetsch P et al. Analysis of expression of the melanoma-associated antigens MART-1 and gp100 in metastatic melanoma cell lines and in in situ lesions. J Immunother Emphasis Tumor Immunol. 1996 May [PMID: 8811494]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP2-15197) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33148AF350
Alexa Fluor 405 NBP2-33148AF405
Alexa Fluor 488 NBP2-33148AF488
Alexa Fluor 532 NBP2-33148AF532
Alexa Fluor 594 NBP2-33148AF594
Alexa Fluor 647 NBP2-33148AF647
Alexa Fluor 700 NBP2-33148AF700
Alexa Fluor 750 NBP2-33148AF750
Biotin NBP2-33148B
DyLight 350 NBP2-33148UV
DyLight 405 NBP2-33148V
DyLight 488 NBP2-33148G
DyLight 550 NBP2-33148R
DyLight 650 NBP2-33148C
DyLight 680 NBP2-33148FR
DyLight 755 NBP2-33148IR
HRP NBP2-33148H
Janelia Fluor 549 NBP2-33148JF549
Janelia Fluor 646 NBP2-33148JF646
PerCP NBP2-33148PCP

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Bioinformatics

Gene Symbol MLANA
Entrez
Uniprot