Melan-A/MART-1 Antibody (M2-7C10)

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.

Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.

Western Blot Analysis of Colo-38 lysate using Melan-A/MART-1 Antibody (M2-7C10).

Western Blot Analysis of Colo-38 lysate using Melan-A/MART-1 Antibody (M2-7C10).

Western Blot Analysis of Colo-38 lysate using Melan-A/MART-1 Antibody (M2-7C10).

• Reactivity: Hu, Eq, Rt(-)
• Applications: WB, Flow, IHC
• Clone: M2-7C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Melan-A/MART-1 Antibody (M2-7C10) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33148)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 0.5-1ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Melan-A/MART-1 Antibody (M2-7C10)

• Antigen LB39-AA
• Antigen SK29-AA
• Mart 1 Melan A
• MART-1
• MART1MART-1
• MelanA
• Melan-A
• melanoma antigen recognized by T-cells 1
• MLANA
• Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognize by T cells 1), also known as Melan-A, is an 18 kDa melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. MART-1 is expressed in melanosomes and the endoplasmic reticulum. MART-1 is the most widely used marker for identifying malignant melanoma, the most deadly form of skin cancer, and facilitating complete removal of the primary tumor (Campoli, 2012). In this regard, MART-1 is used both as a confirmatory marker for melanocyte differentiation in S100 (protein present in melanocytes) positive lesions and a primary marker to evaluate the extent of melanocyte tumors (Ohsie, 2012, Collins, 2012). MART-1 specific monoclonal antibodies have high sensitivity (75-92%) and specificity (95-100%) for melanoma (Campoli, 2012, Oshie et.al, 2012).
The clone M2-7C10 MART-1 antibody labels melanomas and other tumors showing melanocyte differentiation, and is widely used for assessing melanomas (Campoli, 2012, Ohsie, 2012, Collins, 2012). Analysis of melanoma lesions with this antibody shows that there is significant heterogeneity of expression of MART-1 both as a percentage of cells and in intensity of expression (Marincola, 1996). The reactivity of the MART-1 antibody is not restricted to melanoma, and the antibody has also been shown to label some mesenchymal tumors and sarcomas (Campoli, 2012).
The exact eptiope recognized by this MART-1 antibody has not been mapped. However, the MART-1 epitope recognized by this antibody appears to be different from that recognized by the M2-9E3 MART-1 antibody clone (ABX-126A) (Kawakami, 1997). Researchers often use more than one antibody against a given specificity to help follow up and validate results. Hence, it may be useful to use both the ABX-125A and ABX-126A antibodies in parallel to obtain additional information about MART-1 expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melan-A/MART-1 Antibody (NBP2-15197)(11)

Publications using NBP2-15197

• Lu C, Li Y, Chen R et al. Safety, Immunogenicity, and Mechanism of a Rotavirus mRNA-LNP Vaccine in Mice Viruses 2024-01-31 [PMID: 38399987]
• Cruz N, Buscaglia R, Salanga M, Kellar R. Environmentally Relevant Levels of Depleted Uranium Impacts Dermal Fibroblast Proliferation, Viability, Metabolic Activity, and Scratch Closure Toxics 2021-09-03 [PMID: 34564362]
• Loreth D, Schuette M, Zinke J et al. CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis International Journal of Molecular Sciences 2021-06-29 [PMID: 34209696]
• Yang S, Wei J, Cui YH et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade Nat Commun. 2019-06-25 [PMID: 31239444] (IF/IHC, Human)
• Prakash, R;Izraely, S;Thareja, NS;Lee, RH;Rappaport, M;Kawaguchi, R;Sagi-Assif, O;Ben-Menachem, S;Meshel, T;Machnicki, M;Ohe, S;Hoon, DS;Coppola, G;Witz, IP;Carmichael, ST; Regeneration Enhances Metastasis: A Novel Role for Neurovascular Signaling in Promoting Melanoma Brain Metastasis Front Neurosci 2019-04-09 [PMID: 31024232] (IF/IHC, Mouse)
• Collins GR, Essary L, Strauss J et al. Incidentally discovered distant cutaneous metastasis of sacral chordoma: a case with variation in S100 protein expression (compared to the primary tumor) and review of the literature. J Cutan Pathol. 2012-06-01 [PMID: 22524546]
• Mihic-Probst D, Ikenberg K, Tinguely M et al. Tumor cell plasticity and angiogenesis in human melanomas. PLoS One. 2012-01-01 [PMID: 22442699]
• Hoashi T, Watabe H, Muller J et al. MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes. J Biol Chem. 2005-04-08 [PMID: 15695812]
• Puri N, Eller M S et al. Telomere-based DNA damage responses: a new approach to melanoma. FASEB J 2004-01-09 [PMID: 15333580] (WB, Mouse)
• Kawakami Y, Battles JK, Kobayashi T et al. Production of recombinant MART-1 proteins and specific antiMART-1 polyclonal and monoclonal antibodies: use in the characterization of the human melanoma antigen MART-1. J Immunol Methods. 1997-03-10 [PMID: 9075767]
• Marincola FM, Hijazi YM, Fetsch P et al. Analysis of expression of the melanoma-associated antigens MART-1 and gp100 in metastatic melanoma cell lines and in in situ lesions. J Immunother Emphasis Tumor Immunol. 1996-05-01 [PMID: 8811494]

Bioinformatics

• Gene Symbol: MLANA
• Entrez:
  • 2315(Human)
  • 77836(Mouse)
  • 293890(Rat)
• Uniprot:
  • Q16655()
  • Q16655()
  • Q16655()