Recombinant Human MAGEA3 His Protein

SDS-Page: Recombinant Human MAGEA3 Protein [NBP1-49445] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human MAGEA3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-314 of Human MAGEA3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPLEQRS QHCKPEEGLE ARGEALGLVG AQAPATEEQE AASSSSTLVE VTLGEVPAAE SPDPPQSPQG ASSLPTTMNY PLWSQSYEDS SNQEEEGPST FPDLESEFQA ALSRKVAELV HFLLLKYRAR EPVTKAEMLG SVVGNWQYFF PVIFSKASSS LQLVFGIELM EVDPIGHLYI FATCLGLSYD GLLGDNQIMP KAGLLIIVLA IIAREGDCAP EEKIWEELSV LEVFEGREDS ILGDPKKLLT QHFVQENYLE YRQVPGSDPA CYEFLWGPRA LVETSYVKVL HHMVKISGGP HISYPPLHEW VLREGEE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MAGEA3
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 37.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 100 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MAGEA3 His Protein

• Antigen MZ2-D
• Cancer/testis antigen 1.3
• CT1.3 MAGE-3 antigen
• CT1.3
• HIP8
• MAGE3 MAGEA6
• MAGE3
• MAGEA3
• melanoma antigen family A, 3
• melanoma-associated antigen 3
• member 3
• MGC14613

Background

Melanoma-associated antigen 3, also known MAGEA3, is a member of the MAGE gene family, which comprises 12 known genes, of which 6 are expressed in tumors. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGEA3 is a tumor-specific antigen widely expressed in solid and hematologic malignancies, but not in normal tissues, with the exception of testis and placenta. Therefore, MAGEA3 is an excellent candidate tumor antigen. Recombinant human MAGEA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA3