Melan-A/MART-1 Antibody (M2-7C10) [Alexa Fluor® 488]

Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) [Alexa Fluor® 488] [NBP2-33148AF488] - Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.

• Reactivity: Hu, Eq, Rt(-)
• Applications: WB, Flow, IHC
• Clone: M2-7C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Melan-A/MART-1 Antibody (M2-7C10) [Alexa Fluor® 488] Summary

• Immunogen: Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Melan-A/MART-1 Antibody (M2-7C10) [Alexa Fluor® 488]

• Antigen LB39-AA
• Antigen SK29-AA
• Mart 1 Melan A
• MART-1
• MART1MART-1
• MelanA
• Melan-A
• melanoma antigen recognized by T-cells 1
• MLANA
• Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melan-A/MART-1 Antibody (NBP2-33148AF488)(1)

Publication using NBP2-33148AF488

• Loreth D, Schuette M, Zinke J et al. CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis International Journal of Molecular Sciences 2021-06-29 [PMID: 34209696]

Review for Melan-A/MART-1 Antibody (NBP2-33148AF488) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 07/19/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen human skin sections
• Species: Human
• Lot: 2315-1PABX190425-060519AF488

Bioinformatics

• Gene Symbol: MLANA
• Entrez:
  • 2315(Human)
  • 77836(Mouse)
  • 293890(Rat)